The Green Tea Polyphenol Epigallocatechin-Gallate (EGCG) Interferes with Microcin E492 Amyloid Formation
Microcin E492 (MccE492) is an antimicrobial peptide and proposed virulence factor produced by some <i>Klebsiella pneumoniae</i> strains, which, under certain conditions, form amyloid fibers, leading to the loss of its antibacterial activity. Although this protein has been characterized a...
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MDPI AG
2023-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/21/7262 |
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| author | Paulina Aguilera Camilo Berríos-Pastén Marcelo Veloso Matías Gálvez-Silva Florian Turbant Rosalba Lagos Frank Wien Veronique Arluison Andrés E. Marcoleta |
| author_facet | Paulina Aguilera Camilo Berríos-Pastén Marcelo Veloso Matías Gálvez-Silva Florian Turbant Rosalba Lagos Frank Wien Veronique Arluison Andrés E. Marcoleta |
| author_sort | Paulina Aguilera |
| collection | DOAJ |
| description | Microcin E492 (MccE492) is an antimicrobial peptide and proposed virulence factor produced by some <i>Klebsiella pneumoniae</i> strains, which, under certain conditions, form amyloid fibers, leading to the loss of its antibacterial activity. Although this protein has been characterized as a model functional amyloid, the secondary structure transitions behind its formation, and the possible effect of molecules that inhibit this process, have not been investigated. In this study, we examined the ability of the green tea flavonoid epigallocatechin gallate (EGCG) to interfere with MccE492 amyloid formation. Aggregation kinetics followed by thioflavin T binding were used to monitor amyloid formation in the presence or absence of EGCG. Additionally, synchrotron radiation circular dichroism (SRCD) and transmission electron microscopy (TEM) were used to study the secondary structure, thermal stability, and morphology of microcin E492 fibers. Our results showed that EGCG significantly inhibited the formation of the MccE492 amyloid, resulting in mainly amorphous aggregates and small oligomers. However, these aggregates retained part of the β-sheet SRCD signal and a high resistance to heat denaturation, suggesting that the aggregation process is sequestered or deviated at some stage but not completely prevented. Thus, EGCG is an interesting inhibitor of the amyloid formation of MccE492 and other bacterial amyloids. |
| first_indexed | 2024-03-11T11:25:38Z |
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| id | doaj.art-b678fe453866461d907199265ca091c3 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T11:25:38Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b678fe453866461d907199265ca091c32023-11-10T15:08:24ZengMDPI AGMolecules1420-30492023-10-012821726210.3390/molecules28217262The Green Tea Polyphenol Epigallocatechin-Gallate (EGCG) Interferes with Microcin E492 Amyloid FormationPaulina Aguilera0Camilo Berríos-Pastén1Marcelo Veloso2Matías Gálvez-Silva3Florian Turbant4Rosalba Lagos5Frank Wien6Veronique Arluison7Andrés E. Marcoleta8Grupo de Microbiología Integrativa, Laboratorio de Biología Estructural y Molecular BEM, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425 Ñuñoa, Santiago 7800003, ChileGrupo de Microbiología Integrativa, Laboratorio de Biología Estructural y Molecular BEM, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425 Ñuñoa, Santiago 7800003, ChileGrupo de Microbiología Integrativa, Laboratorio de Biología Estructural y Molecular BEM, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425 Ñuñoa, Santiago 7800003, ChileGrupo de Microbiología Integrativa, Laboratorio de Biología Estructural y Molecular BEM, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425 Ñuñoa, Santiago 7800003, ChileSynchrotron SOLEIL, L’Orme des Merisiers, Saint Aubin BP48, 91192 Gif-sur-Yvette, FranceGrupo de Microbiología Integrativa, Laboratorio de Biología Estructural y Molecular BEM, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425 Ñuñoa, Santiago 7800003, ChileSynchrotron SOLEIL, L’Orme des Merisiers, Saint Aubin BP48, 91192 Gif-sur-Yvette, FranceLaboratoire Léon Brillouin LLB, CEA, CNRS UMR12, Université Paris-Saclay, CEA Saclay, 91191 Gif-sur-Yvette, FranceGrupo de Microbiología Integrativa, Laboratorio de Biología Estructural y Molecular BEM, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425 Ñuñoa, Santiago 7800003, ChileMicrocin E492 (MccE492) is an antimicrobial peptide and proposed virulence factor produced by some <i>Klebsiella pneumoniae</i> strains, which, under certain conditions, form amyloid fibers, leading to the loss of its antibacterial activity. Although this protein has been characterized as a model functional amyloid, the secondary structure transitions behind its formation, and the possible effect of molecules that inhibit this process, have not been investigated. In this study, we examined the ability of the green tea flavonoid epigallocatechin gallate (EGCG) to interfere with MccE492 amyloid formation. Aggregation kinetics followed by thioflavin T binding were used to monitor amyloid formation in the presence or absence of EGCG. Additionally, synchrotron radiation circular dichroism (SRCD) and transmission electron microscopy (TEM) were used to study the secondary structure, thermal stability, and morphology of microcin E492 fibers. Our results showed that EGCG significantly inhibited the formation of the MccE492 amyloid, resulting in mainly amorphous aggregates and small oligomers. However, these aggregates retained part of the β-sheet SRCD signal and a high resistance to heat denaturation, suggesting that the aggregation process is sequestered or deviated at some stage but not completely prevented. Thus, EGCG is an interesting inhibitor of the amyloid formation of MccE492 and other bacterial amyloids.https://www.mdpi.com/1420-3049/28/21/7262microcin E492bacterial amyloidEGCGgreen teasynchrotron-radiation circular dichroism |
| spellingShingle | Paulina Aguilera Camilo Berríos-Pastén Marcelo Veloso Matías Gálvez-Silva Florian Turbant Rosalba Lagos Frank Wien Veronique Arluison Andrés E. Marcoleta The Green Tea Polyphenol Epigallocatechin-Gallate (EGCG) Interferes with Microcin E492 Amyloid Formation Molecules microcin E492 bacterial amyloid EGCG green tea synchrotron-radiation circular dichroism |
| title | The Green Tea Polyphenol Epigallocatechin-Gallate (EGCG) Interferes with Microcin E492 Amyloid Formation |
| title_full | The Green Tea Polyphenol Epigallocatechin-Gallate (EGCG) Interferes with Microcin E492 Amyloid Formation |
| title_fullStr | The Green Tea Polyphenol Epigallocatechin-Gallate (EGCG) Interferes with Microcin E492 Amyloid Formation |
| title_full_unstemmed | The Green Tea Polyphenol Epigallocatechin-Gallate (EGCG) Interferes with Microcin E492 Amyloid Formation |
| title_short | The Green Tea Polyphenol Epigallocatechin-Gallate (EGCG) Interferes with Microcin E492 Amyloid Formation |
| title_sort | green tea polyphenol epigallocatechin gallate egcg interferes with microcin e492 amyloid formation |
| topic | microcin E492 bacterial amyloid EGCG green tea synchrotron-radiation circular dichroism |
| url | https://www.mdpi.com/1420-3049/28/21/7262 |
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