Immunoinformatic approach for the evaluation of sortase C and E proteins as vaccine targets against caseous lymphadenitis
Corynebacterium pseudotuberculosis, a facultative intracellular pathogen, causes caseous lymphadenitis, a disease of veterinary importance in small ruminants. In order to reduce the economic losses caused by the disease, an effective prophylactic measure is necessary. We adopted an immunoinformatic...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-01-01
|
| Series: | Informatics in Medicine Unlocked |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352914821001982 |
| _version_ | 1818929035734941696 |
|---|---|
| author | Felipe Moura Silva Marcelo dos Santos Barbosa Sandeep Tiwari Nubia Seyffert Vasco Ariston de Carvalho Azevedo Roberto José Meyer Nascimento Thiago Luiz de Paula Castro Silvana Beutinger Marchioro |
| author_facet | Felipe Moura Silva Marcelo dos Santos Barbosa Sandeep Tiwari Nubia Seyffert Vasco Ariston de Carvalho Azevedo Roberto José Meyer Nascimento Thiago Luiz de Paula Castro Silvana Beutinger Marchioro |
| author_sort | Felipe Moura Silva |
| collection | DOAJ |
| description | Corynebacterium pseudotuberculosis, a facultative intracellular pathogen, causes caseous lymphadenitis, a disease of veterinary importance in small ruminants. In order to reduce the economic losses caused by the disease, an effective prophylactic measure is necessary. We adopted an immunoinformatic approach to evaluate the immunological profile of sortases C and E. For this, we performed homology analysis, searched for T and B cell epitopes, evaluated immunogenic capacity, toxicity and allergenicity, and the conservation of these epitopes in different C. pseudotuberculosis strains. The tertiary structure of the protein was predicted, and molecular docking analysis with TLR-2 was performed. Physicochemical parameters of the two sortases were also evaluated. Sortases C and E were observed to produce cellular immunity through specific epitopes for T lymphocytes, humoral immunity through epitopes of B lymphocytes, and also exhibited a high binding capacity to toll-like receptor 2. We filtered and selected six sortase C and two sortase E non-toxic and non-allergenic epitopes and confirmed their interaction with the OVAR-N*0201 allele of sheep. Our results indicate that sortase C and E have important epitopes that participate in the course of C. pseudotuberculosis infection and can serve as immunobiological candidates in the prevention of caseous lymphadenitis. |
| first_indexed | 2024-12-20T03:38:24Z |
| format | Article |
| id | doaj.art-b67a6de1ec5a4bc68166b4b900564984 |
| institution | Directory Open Access Journal |
| issn | 2352-9148 |
| language | English |
| last_indexed | 2024-12-20T03:38:24Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Informatics in Medicine Unlocked |
| spelling | doaj.art-b67a6de1ec5a4bc68166b4b9005649842022-12-21T19:54:48ZengElsevierInformatics in Medicine Unlocked2352-91482021-01-0126100718Immunoinformatic approach for the evaluation of sortase C and E proteins as vaccine targets against caseous lymphadenitisFelipe Moura Silva0Marcelo dos Santos Barbosa1Sandeep Tiwari2Nubia Seyffert3Vasco Ariston de Carvalho Azevedo4Roberto José Meyer Nascimento5Thiago Luiz de Paula Castro6Silvana Beutinger Marchioro7Immunology Laboratory, Health Science Institute, Federal University of Bahia, Salvador, Bahia, BrazilFederal University of Grande Dourados, Faculty of Health Sciences, Dourados, MS, BrazilDepartment of Genetics, Ecology and Evolution, Federal University of Minas Gerais, Minas Gerais, BrazilImmunology Laboratory, Health Science Institute, Federal University of Bahia, Salvador, Bahia, Brazil; Department of Genetics, Ecology and Evolution, Federal University of Minas Gerais, Minas Gerais, BrazilDepartment of Genetics, Ecology and Evolution, Federal University of Minas Gerais, Minas Gerais, BrazilImmunology Laboratory, Health Science Institute, Federal University of Bahia, Salvador, Bahia, BrazilImmunology Laboratory, Health Science Institute, Federal University of Bahia, Salvador, Bahia, Brazil; Department of Genetics, Ecology and Evolution, Federal University of Minas Gerais, Minas Gerais, BrazilImmunology Laboratory, Health Science Institute, Federal University of Bahia, Salvador, Bahia, Brazil; Federal University of Grande Dourados, Faculty of Health Sciences, Dourados, MS, Brazil; Corresponding author. Federal University of Bahia, Health Sciences Institute, Salvador, BA, Brazil.Corynebacterium pseudotuberculosis, a facultative intracellular pathogen, causes caseous lymphadenitis, a disease of veterinary importance in small ruminants. In order to reduce the economic losses caused by the disease, an effective prophylactic measure is necessary. We adopted an immunoinformatic approach to evaluate the immunological profile of sortases C and E. For this, we performed homology analysis, searched for T and B cell epitopes, evaluated immunogenic capacity, toxicity and allergenicity, and the conservation of these epitopes in different C. pseudotuberculosis strains. The tertiary structure of the protein was predicted, and molecular docking analysis with TLR-2 was performed. Physicochemical parameters of the two sortases were also evaluated. Sortases C and E were observed to produce cellular immunity through specific epitopes for T lymphocytes, humoral immunity through epitopes of B lymphocytes, and also exhibited a high binding capacity to toll-like receptor 2. We filtered and selected six sortase C and two sortase E non-toxic and non-allergenic epitopes and confirmed their interaction with the OVAR-N*0201 allele of sheep. Our results indicate that sortase C and E have important epitopes that participate in the course of C. pseudotuberculosis infection and can serve as immunobiological candidates in the prevention of caseous lymphadenitis.http://www.sciencedirect.com/science/article/pii/S2352914821001982Corynebacterium pseudotuberculosisCaseous lymphadenitisSortase proteinVaccineImmunoinformatic analysis |
| spellingShingle | Felipe Moura Silva Marcelo dos Santos Barbosa Sandeep Tiwari Nubia Seyffert Vasco Ariston de Carvalho Azevedo Roberto José Meyer Nascimento Thiago Luiz de Paula Castro Silvana Beutinger Marchioro Immunoinformatic approach for the evaluation of sortase C and E proteins as vaccine targets against caseous lymphadenitis Informatics in Medicine Unlocked Corynebacterium pseudotuberculosis Caseous lymphadenitis Sortase protein Vaccine Immunoinformatic analysis |
| title | Immunoinformatic approach for the evaluation of sortase C and E proteins as vaccine targets against caseous lymphadenitis |
| title_full | Immunoinformatic approach for the evaluation of sortase C and E proteins as vaccine targets against caseous lymphadenitis |
| title_fullStr | Immunoinformatic approach for the evaluation of sortase C and E proteins as vaccine targets against caseous lymphadenitis |
| title_full_unstemmed | Immunoinformatic approach for the evaluation of sortase C and E proteins as vaccine targets against caseous lymphadenitis |
| title_short | Immunoinformatic approach for the evaluation of sortase C and E proteins as vaccine targets against caseous lymphadenitis |
| title_sort | immunoinformatic approach for the evaluation of sortase c and e proteins as vaccine targets against caseous lymphadenitis |
| topic | Corynebacterium pseudotuberculosis Caseous lymphadenitis Sortase protein Vaccine Immunoinformatic analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2352914821001982 |
| work_keys_str_mv | AT felipemourasilva immunoinformaticapproachfortheevaluationofsortasecandeproteinsasvaccinetargetsagainstcaseouslymphadenitis AT marcelodossantosbarbosa immunoinformaticapproachfortheevaluationofsortasecandeproteinsasvaccinetargetsagainstcaseouslymphadenitis AT sandeeptiwari immunoinformaticapproachfortheevaluationofsortasecandeproteinsasvaccinetargetsagainstcaseouslymphadenitis AT nubiaseyffert immunoinformaticapproachfortheevaluationofsortasecandeproteinsasvaccinetargetsagainstcaseouslymphadenitis AT vascoaristondecarvalhoazevedo immunoinformaticapproachfortheevaluationofsortasecandeproteinsasvaccinetargetsagainstcaseouslymphadenitis AT robertojosemeyernascimento immunoinformaticapproachfortheevaluationofsortasecandeproteinsasvaccinetargetsagainstcaseouslymphadenitis AT thiagoluizdepaulacastro immunoinformaticapproachfortheevaluationofsortasecandeproteinsasvaccinetargetsagainstcaseouslymphadenitis AT silvanabeutingermarchioro immunoinformaticapproachfortheevaluationofsortasecandeproteinsasvaccinetargetsagainstcaseouslymphadenitis |