Differential cytokine expression in gastric tissues highlights helicobacter pylori’s role in gastritis
Abstract Helicobacter pylori (H. pylori), known for causing gastric inflammation, gastritis and gastric cancer, prompted our study to investigate the differential expression of cytokines in gastric tissues, which is crucial for understanding H. pylori infection and its potential progression to gastr...
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| Format: | Article |
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Nature Portfolio
2024-04-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-58407-x |
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| author | Xing-Tang Yang Pei-Qin Niu Xiao-Feng Li Ming-Ming Sun Wei Wei Yan-Qing Chen Jia-Yi Zheng |
| author_facet | Xing-Tang Yang Pei-Qin Niu Xiao-Feng Li Ming-Ming Sun Wei Wei Yan-Qing Chen Jia-Yi Zheng |
| author_sort | Xing-Tang Yang |
| collection | DOAJ |
| description | Abstract Helicobacter pylori (H. pylori), known for causing gastric inflammation, gastritis and gastric cancer, prompted our study to investigate the differential expression of cytokines in gastric tissues, which is crucial for understanding H. pylori infection and its potential progression to gastric cancer. Focusing on Il-1β, IL-6, IL-8, IL-12, IL-18, and TNF-α, we analysed gene and protein levels to differentiate between H. pylori-infected and non-infected gastritis. We utilised real-time quantitative polymerase chain reaction (RT-qPCR) for gene quantification, immunohistochemical staining, and ELISA for protein measurement. Gastric samples from patients with gastritis were divided into three groups: (1) non-gastritis (N-group) group, (2) gastritis without H. pylori infection (G-group), and (3) gastritis with H. pylori infection (GH-group), each consisting of 8 samples. Our findings revealed a statistically significant variation in cytokine expression. Generally, cytokine levels were higher in gastritis, but in H. pylori-infected gastritis, IL-1β, IL-6, and IL-8 levels were lower compared to H. pylori-independent gastritis, while IL-12, IL-18, and TNF-α levels were higher. This distinct cytokine expression pattern in H. pylori-infected gastritis underscores a unique inflammatory response, providing deeper insights into its pathogenesis. |
| first_indexed | 2024-04-24T12:40:51Z |
| format | Article |
| id | doaj.art-b67b0ff07b3749c5b27970303ad36566 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T12:40:51Z |
| publishDate | 2024-04-01 |
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| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b67b0ff07b3749c5b27970303ad365662024-04-07T11:16:46ZengNature PortfolioScientific Reports2045-23222024-04-0114111210.1038/s41598-024-58407-xDifferential cytokine expression in gastric tissues highlights helicobacter pylori’s role in gastritisXing-Tang Yang0Pei-Qin Niu1Xiao-Feng Li2Ming-Ming Sun3Wei Wei4Yan-Qing Chen5Jia-Yi Zheng6Department of Gastroenterology, Chongming Branch, Shanghai Tenth People’s Hospital, Tongji University School of MedicineDepartment of Gastroenterology, Chongming Branch, Shanghai Tenth People’s Hospital, Tongji University School of MedicineDepartment of Emergency, Shanghai Tenth People’s Hospital, Tongji University School of MedicineDepartment of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University School of MedicineDepartment of Emergency, Shanghai Tenth People’s Hospital, Tongji University School of MedicineDepartment of Emergency, Shanghai Tenth People’s Hospital, Tongji University School of MedicineDepartment of Pathology, Shanghai Tenth People’s Hospital, Tongji University School of MedicineAbstract Helicobacter pylori (H. pylori), known for causing gastric inflammation, gastritis and gastric cancer, prompted our study to investigate the differential expression of cytokines in gastric tissues, which is crucial for understanding H. pylori infection and its potential progression to gastric cancer. Focusing on Il-1β, IL-6, IL-8, IL-12, IL-18, and TNF-α, we analysed gene and protein levels to differentiate between H. pylori-infected and non-infected gastritis. We utilised real-time quantitative polymerase chain reaction (RT-qPCR) for gene quantification, immunohistochemical staining, and ELISA for protein measurement. Gastric samples from patients with gastritis were divided into three groups: (1) non-gastritis (N-group) group, (2) gastritis without H. pylori infection (G-group), and (3) gastritis with H. pylori infection (GH-group), each consisting of 8 samples. Our findings revealed a statistically significant variation in cytokine expression. Generally, cytokine levels were higher in gastritis, but in H. pylori-infected gastritis, IL-1β, IL-6, and IL-8 levels were lower compared to H. pylori-independent gastritis, while IL-12, IL-18, and TNF-α levels were higher. This distinct cytokine expression pattern in H. pylori-infected gastritis underscores a unique inflammatory response, providing deeper insights into its pathogenesis.https://doi.org/10.1038/s41598-024-58407-xGastritisHelicobacter pyloriCytokinesRT-qPCRImmunohistochemical stainingH&E staining |
| spellingShingle | Xing-Tang Yang Pei-Qin Niu Xiao-Feng Li Ming-Ming Sun Wei Wei Yan-Qing Chen Jia-Yi Zheng Differential cytokine expression in gastric tissues highlights helicobacter pylori’s role in gastritis Scientific Reports Gastritis Helicobacter pylori Cytokines RT-qPCR Immunohistochemical staining H&E staining |
| title | Differential cytokine expression in gastric tissues highlights helicobacter pylori’s role in gastritis |
| title_full | Differential cytokine expression in gastric tissues highlights helicobacter pylori’s role in gastritis |
| title_fullStr | Differential cytokine expression in gastric tissues highlights helicobacter pylori’s role in gastritis |
| title_full_unstemmed | Differential cytokine expression in gastric tissues highlights helicobacter pylori’s role in gastritis |
| title_short | Differential cytokine expression in gastric tissues highlights helicobacter pylori’s role in gastritis |
| title_sort | differential cytokine expression in gastric tissues highlights helicobacter pylori s role in gastritis |
| topic | Gastritis Helicobacter pylori Cytokines RT-qPCR Immunohistochemical staining H&E staining |
| url | https://doi.org/10.1038/s41598-024-58407-x |
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