Isolation and characterization of five novel mini-M conotoxins from the venom of mollusk-hunter snail Conus bandanus
Objective: To determine the new M-superfamily conotoxins from molluscivorous snail Conus bandanus in Vietnam. Methods: Conus bandanus venom was fractionated and purified on HPLC system with an analytical reversed-phase C18 column in order to screen small conotoxins. The primary structure of peptide...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2020-01-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=8;spage=343;epage=352;aulast=Bao |
| _version_ | 1818204028989865984 |
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| author | Nguyen Bao Jean-Pière LE CAER Phan Thi Khanh Vinh |
| author_facet | Nguyen Bao Jean-Pière LE CAER Phan Thi Khanh Vinh |
| author_sort | Nguyen Bao |
| collection | DOAJ |
| description | Objective: To determine the new M-superfamily conotoxins from molluscivorous snail Conus bandanus in Vietnam.
Methods: Conus bandanus venom was fractionated and purified on HPLC system with an analytical reversed-phase C18 column in order to screen small conotoxins. The primary structure of peptide was analyzed by matrix-assisted laser desorption/ionization time of flight tandem mass spectrometry using collision-induced dissociation and confirmed by Edman’s degradation method.
Results: Five new conotoxins were biochemically characterized from the crude venom of the mollusk-hunting cone snail Conus bandanus, which were collected at Ke Ga reef of the Nha Trang Bay (Vietnam). Each conotoxin had 15 or 16 amino acid residues and shared the same characteristic cysteine framework V as –CC–C–C–CC–. They were termed as Bn3b, Bn3c, Bn3d, Bn3e and Bn3f following the conotoxins nomenclature.
Conclusions: The conotoxins Bn3b, Bn3e, and Bn3f are categorized in the mini-M conotoxins of the M1 branch, while conotoxins Bn3c and Bn3d are categorized in the mini-M conotoxins of the M2 branch. The homological analysis reveals that these conotoxins could serve as promising probe compounds for voltage-gated sodium channels. |
| first_indexed | 2024-12-12T03:34:44Z |
| format | Article |
| id | doaj.art-b67cf2eb83664ef6b3d0bc086186ccd3 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 2588-9222 |
| language | English |
| last_indexed | 2024-12-12T03:34:44Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-b67cf2eb83664ef6b3d0bc086186ccd32022-12-22T00:39:50ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222020-01-0110834335210.4103/2221-1691.287161Isolation and characterization of five novel mini-M conotoxins from the venom of mollusk-hunter snail Conus bandanusNguyen BaoJean-Pière LE CAERPhan Thi Khanh VinhObjective: To determine the new M-superfamily conotoxins from molluscivorous snail Conus bandanus in Vietnam. Methods: Conus bandanus venom was fractionated and purified on HPLC system with an analytical reversed-phase C18 column in order to screen small conotoxins. The primary structure of peptide was analyzed by matrix-assisted laser desorption/ionization time of flight tandem mass spectrometry using collision-induced dissociation and confirmed by Edman’s degradation method. Results: Five new conotoxins were biochemically characterized from the crude venom of the mollusk-hunting cone snail Conus bandanus, which were collected at Ke Ga reef of the Nha Trang Bay (Vietnam). Each conotoxin had 15 or 16 amino acid residues and shared the same characteristic cysteine framework V as –CC–C–C–CC–. They were termed as Bn3b, Bn3c, Bn3d, Bn3e and Bn3f following the conotoxins nomenclature. Conclusions: The conotoxins Bn3b, Bn3e, and Bn3f are categorized in the mini-M conotoxins of the M1 branch, while conotoxins Bn3c and Bn3d are categorized in the mini-M conotoxins of the M2 branch. The homological analysis reveals that these conotoxins could serve as promising probe compounds for voltage-gated sodium channels.http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=8;spage=343;epage=352;aulast=Baomini-m conotoxin; conus bandanus; cone snail venom |
| spellingShingle | Nguyen Bao Jean-Pière LE CAER Phan Thi Khanh Vinh Isolation and characterization of five novel mini-M conotoxins from the venom of mollusk-hunter snail Conus bandanus Asian Pacific Journal of Tropical Biomedicine mini-m conotoxin; conus bandanus; cone snail venom |
| title | Isolation and characterization of five novel mini-M conotoxins from the venom of mollusk-hunter snail Conus bandanus |
| title_full | Isolation and characterization of five novel mini-M conotoxins from the venom of mollusk-hunter snail Conus bandanus |
| title_fullStr | Isolation and characterization of five novel mini-M conotoxins from the venom of mollusk-hunter snail Conus bandanus |
| title_full_unstemmed | Isolation and characterization of five novel mini-M conotoxins from the venom of mollusk-hunter snail Conus bandanus |
| title_short | Isolation and characterization of five novel mini-M conotoxins from the venom of mollusk-hunter snail Conus bandanus |
| title_sort | isolation and characterization of five novel mini m conotoxins from the venom of mollusk hunter snail conus bandanus |
| topic | mini-m conotoxin; conus bandanus; cone snail venom |
| url | http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=8;spage=343;epage=352;aulast=Bao |
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