Molecular characterization of a defender against apoptotic cell death 1 gene (CfDAD1) from the mollusk Chlamys farreri
The defender against apoptotic cell death 1 (DAD1) was a negative regulator to inhibit the apoptosis process. In the present study, a DAD1 gene (designated as CfDAD1) was identified in Zhikong scallop Chlamys farreri. Its full-length cDNA sequence of CfDAD1 contained a 342 bp open reading frame (ORF...
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| Format: | Article |
| Language: | English |
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University of Modena and Reggio Emilia
2018-08-01
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| Series: | Invertebrate Survival Journal |
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| Online Access: | https://isj02.unimore.it/index.php/ISJ/article/view/435 |
| _version_ | 1818866050028011520 |
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| author | M Q Wang B J Wang M Liu K Y Jiang L Wang |
| author_facet | M Q Wang B J Wang M Liu K Y Jiang L Wang |
| author_sort | M Q Wang |
| collection | DOAJ |
| description | The defender against apoptotic cell death 1 (DAD1) was a negative regulator to inhibit the apoptosis process. In the present study, a DAD1 gene (designated as CfDAD1) was identified in
Zhikong scallop Chlamys farreri. Its full-length cDNA sequence of CfDAD1 contained a 342 bp open reading frame (ORF), which encoded a mature protein of 113 amino acids. A DAD domain was revealed from the deduced protein sequence of CfDAD1. The mRNA transcripts of CfDAD1 could be detectable in all the investigated tissues, including hemocytes, muscle, mantle, gill, hepatopancreas and gonad, among which the maximum expression level were found in hemocytes. Being infected with Vibrio splendidus, Staphylococcus aureus or Yarrowia lipolytica, the mRNA expression levels of CfDAD1 in hemocytes were all significantly up-regulated. Moreover, the apoptosis level of CfDAD1-suppressed group was significant higher than those of control groups. All these results indicated that CfDAD1 was efficient negative regulator of apoptosis and involved in the innate immune responses of scallop. |
| first_indexed | 2024-12-19T10:57:16Z |
| format | Article |
| id | doaj.art-b67d7188cc2c4101a0a518cd9846e6c8 |
| institution | Directory Open Access Journal |
| issn | 1824-307X |
| language | English |
| last_indexed | 2024-12-19T10:57:16Z |
| publishDate | 2018-08-01 |
| publisher | University of Modena and Reggio Emilia |
| record_format | Article |
| series | Invertebrate Survival Journal |
| spelling | doaj.art-b67d7188cc2c4101a0a518cd9846e6c82022-12-21T20:24:45ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2018-08-01151Molecular characterization of a defender against apoptotic cell death 1 gene (CfDAD1) from the mollusk Chlamys farreriM Q Wang0B J Wang1M Liu2K Y Jiang3L Wang4CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China ; Research Platform for Marine Molecular Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China ; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China ; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266400, ChinaThe defender against apoptotic cell death 1 (DAD1) was a negative regulator to inhibit the apoptosis process. In the present study, a DAD1 gene (designated as CfDAD1) was identified in Zhikong scallop Chlamys farreri. Its full-length cDNA sequence of CfDAD1 contained a 342 bp open reading frame (ORF), which encoded a mature protein of 113 amino acids. A DAD domain was revealed from the deduced protein sequence of CfDAD1. The mRNA transcripts of CfDAD1 could be detectable in all the investigated tissues, including hemocytes, muscle, mantle, gill, hepatopancreas and gonad, among which the maximum expression level were found in hemocytes. Being infected with Vibrio splendidus, Staphylococcus aureus or Yarrowia lipolytica, the mRNA expression levels of CfDAD1 in hemocytes were all significantly up-regulated. Moreover, the apoptosis level of CfDAD1-suppressed group was significant higher than those of control groups. All these results indicated that CfDAD1 was efficient negative regulator of apoptosis and involved in the innate immune responses of scallop.https://isj02.unimore.it/index.php/ISJ/article/view/435ApoptosisChlamys farreriInnate immunity |
| spellingShingle | M Q Wang B J Wang M Liu K Y Jiang L Wang Molecular characterization of a defender against apoptotic cell death 1 gene (CfDAD1) from the mollusk Chlamys farreri Invertebrate Survival Journal Apoptosis Chlamys farreri Innate immunity |
| title | Molecular characterization of a defender against apoptotic cell death 1 gene (CfDAD1) from the mollusk Chlamys farreri |
| title_full | Molecular characterization of a defender against apoptotic cell death 1 gene (CfDAD1) from the mollusk Chlamys farreri |
| title_fullStr | Molecular characterization of a defender against apoptotic cell death 1 gene (CfDAD1) from the mollusk Chlamys farreri |
| title_full_unstemmed | Molecular characterization of a defender against apoptotic cell death 1 gene (CfDAD1) from the mollusk Chlamys farreri |
| title_short | Molecular characterization of a defender against apoptotic cell death 1 gene (CfDAD1) from the mollusk Chlamys farreri |
| title_sort | molecular characterization of a defender against apoptotic cell death 1 gene cfdad1 from the mollusk chlamys farreri |
| topic | Apoptosis Chlamys farreri Innate immunity |
| url | https://isj02.unimore.it/index.php/ISJ/article/view/435 |
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