Lobaplatin arrests cell cycle progression in human hepatocellular carcinoma cells
<p>Abstract</p> <p>Background</p> <p>Hepatocellular carcinoma (HCC) still is a big burden for China. In recent years, the third-generation platinum compounds have been proposed as potential active agents for HCC. However, more experimental and clinical data are warrante...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-10-01
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| Series: | Journal of Hematology & Oncology |
| Online Access: | http://www.jhoonline.org/content/3/1/43 |
| _version_ | 1819182078744330240 |
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| author | Chen Chang-Jie Teng Feng-Meng Qin Shu-Kui Wu Qiong Wang Rui |
| author_facet | Chen Chang-Jie Teng Feng-Meng Qin Shu-Kui Wu Qiong Wang Rui |
| author_sort | Chen Chang-Jie |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Hepatocellular carcinoma (HCC) still is a big burden for China. In recent years, the third-generation platinum compounds have been proposed as potential active agents for HCC. However, more experimental and clinical data are warranted to support the proposal. In the present study, the effect of lobaplatin was assessed in five HCC cell lines and the underlying molecular mechanisms in terms of cell cycle kinetics were explored.</p> <p>Methods</p> <p>Cytotoxicity of lobaplatin to human HCC cell lines was examined using MTT cell proliferation assay. Cell cycle distribution was determined by flow cytometry. Expression of cell cycle-regulated genes was examined at both the mRNA (RT-PCR) and protein (Western blot) levels. The phosphorylation status of cyclin-dependent kinases (CDKs) and retinoblastoma (Rb) protein was also examined using Western blot analysis.</p> <p>Results</p> <p>Lobaplatin inhibited proliferation of human HCC cells in a dose-dependent manner. For the most sensitive SMMC-7721 cells, lobaplatin arrested cell cycle progression in G<sub>1 </sub>and G<sub>2</sub>/M phases time-dependently which might be associated with the down-regulation of cyclin B, CDK1, CDC25C, phosphorylated CDK1 (pCDK1), pCDK4, Rb, E2F, and pRb, and the up-regulation of p53, p21, and p27.</p> <p>Conclusion</p> <p>Cytotoxicity of lobaplatin in human HCC cells might be due to its ability to arrest cell cycle progression which would contribute to the potential use of lobaplatin for the management of HCC.</p> |
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| format | Article |
| id | doaj.art-b67e8bf5a2df404ebfc44557d88658cf |
| institution | Directory Open Access Journal |
| issn | 1756-8722 |
| language | English |
| last_indexed | 2024-12-22T22:40:25Z |
| publishDate | 2010-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Hematology & Oncology |
| spelling | doaj.art-b67e8bf5a2df404ebfc44557d88658cf2022-12-21T18:10:12ZengBMCJournal of Hematology & Oncology1756-87222010-10-01314310.1186/1756-8722-3-43Lobaplatin arrests cell cycle progression in human hepatocellular carcinoma cellsChen Chang-JieTeng Feng-MengQin Shu-KuiWu QiongWang Rui<p>Abstract</p> <p>Background</p> <p>Hepatocellular carcinoma (HCC) still is a big burden for China. In recent years, the third-generation platinum compounds have been proposed as potential active agents for HCC. However, more experimental and clinical data are warranted to support the proposal. In the present study, the effect of lobaplatin was assessed in five HCC cell lines and the underlying molecular mechanisms in terms of cell cycle kinetics were explored.</p> <p>Methods</p> <p>Cytotoxicity of lobaplatin to human HCC cell lines was examined using MTT cell proliferation assay. Cell cycle distribution was determined by flow cytometry. Expression of cell cycle-regulated genes was examined at both the mRNA (RT-PCR) and protein (Western blot) levels. The phosphorylation status of cyclin-dependent kinases (CDKs) and retinoblastoma (Rb) protein was also examined using Western blot analysis.</p> <p>Results</p> <p>Lobaplatin inhibited proliferation of human HCC cells in a dose-dependent manner. For the most sensitive SMMC-7721 cells, lobaplatin arrested cell cycle progression in G<sub>1 </sub>and G<sub>2</sub>/M phases time-dependently which might be associated with the down-regulation of cyclin B, CDK1, CDC25C, phosphorylated CDK1 (pCDK1), pCDK4, Rb, E2F, and pRb, and the up-regulation of p53, p21, and p27.</p> <p>Conclusion</p> <p>Cytotoxicity of lobaplatin in human HCC cells might be due to its ability to arrest cell cycle progression which would contribute to the potential use of lobaplatin for the management of HCC.</p>http://www.jhoonline.org/content/3/1/43 |
| spellingShingle | Chen Chang-Jie Teng Feng-Meng Qin Shu-Kui Wu Qiong Wang Rui Lobaplatin arrests cell cycle progression in human hepatocellular carcinoma cells Journal of Hematology & Oncology |
| title | Lobaplatin arrests cell cycle progression in human hepatocellular carcinoma cells |
| title_full | Lobaplatin arrests cell cycle progression in human hepatocellular carcinoma cells |
| title_fullStr | Lobaplatin arrests cell cycle progression in human hepatocellular carcinoma cells |
| title_full_unstemmed | Lobaplatin arrests cell cycle progression in human hepatocellular carcinoma cells |
| title_short | Lobaplatin arrests cell cycle progression in human hepatocellular carcinoma cells |
| title_sort | lobaplatin arrests cell cycle progression in human hepatocellular carcinoma cells |
| url | http://www.jhoonline.org/content/3/1/43 |
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