Biotransformation of (–)-Isopulegol by <i>Rhodococcus rhodochrous</i>
The ability of actinobacteria of the genus <i>Rhodococcus</i> to biotransform the monoterpenoid (–)-isopulegol has been established for the first time. <i>R</i>. <i>rhodochrous</i> strain IEGM 1362 was selected as a bacterium capable of metabolizing (–)-isopulegol...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/15/8/964 |
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| author | Irina B. Ivshina Natalia A. Luchnikova Polina Yu. Maltseva Irina V. Ilyina Konstantin P. Volcho Yurii V. Gatilov Dina V. Korchagina Nadezhda A. Kostrikina Vladimir V. Sorokin Andrey L. Mulyukin Nariman F. Salakhutdinov |
| author_facet | Irina B. Ivshina Natalia A. Luchnikova Polina Yu. Maltseva Irina V. Ilyina Konstantin P. Volcho Yurii V. Gatilov Dina V. Korchagina Nadezhda A. Kostrikina Vladimir V. Sorokin Andrey L. Mulyukin Nariman F. Salakhutdinov |
| author_sort | Irina B. Ivshina |
| collection | DOAJ |
| description | The ability of actinobacteria of the genus <i>Rhodococcus</i> to biotransform the monoterpenoid (–)-isopulegol has been established for the first time. <i>R</i>. <i>rhodochrous</i> strain IEGM 1362 was selected as a bacterium capable of metabolizing (–)-isopulegol to form new, previously unknown, 10-hydroxy (<b>2</b>) and 10-carboxy (<b>3</b>) derivatives, which may presumably have antitumor activity and act as respiratory stimulants and cancer prevention agents. In the experiments, optimal conditions were selected to provide the maximum target catalytic activity of rhodococci. Using up-to-date (TEM, AFM-CLSM, and EDX) and traditional (cell size, roughness, and zeta potential measurements) biophysical and microbiological methods, it was shown that (–)-isopulegol and halloysite nanotubes did not negatively affect the bacterial cells. The data obtained expand our knowledge of the biocatalytic potential of rhodococci and their possible involvement in the synthesis of pharmacologically active compounds from plant derivatives. |
| first_indexed | 2024-03-09T03:58:47Z |
| format | Article |
| id | doaj.art-b67f09e12dc44c219c1d7906aca042ab |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T03:58:47Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-b67f09e12dc44c219c1d7906aca042ab2023-12-03T14:16:15ZengMDPI AGPharmaceuticals1424-82472022-08-0115896410.3390/ph15080964Biotransformation of (–)-Isopulegol by <i>Rhodococcus rhodochrous</i>Irina B. Ivshina0Natalia A. Luchnikova1Polina Yu. Maltseva2Irina V. Ilyina3Konstantin P. Volcho4Yurii V. Gatilov5Dina V. Korchagina6Nadezhda A. Kostrikina7Vladimir V. Sorokin8Andrey L. Mulyukin9Nariman F. Salakhutdinov10Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences, Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences, 13 Golev Str., 614081 Perm, RussiaInstitute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences, Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences, 13 Golev Str., 614081 Perm, RussiaDepartment of Microbiology and Immunology, Perm State National Research University, 15 Bukirev Str., 614990 Perm, RussiaN.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of the Russian Academy of Sciences, 9 Lavrentiev Avenue, 630090 Novosibirsk, RussiaN.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of the Russian Academy of Sciences, 9 Lavrentiev Avenue, 630090 Novosibirsk, RussiaN.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of the Russian Academy of Sciences, 9 Lavrentiev Avenue, 630090 Novosibirsk, RussiaN.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of the Russian Academy of Sciences, 9 Lavrentiev Avenue, 630090 Novosibirsk, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 60 let Oktyabrya, 7, bld. 2, 117312 Moscow, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 60 let Oktyabrya, 7, bld. 2, 117312 Moscow, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 60 let Oktyabrya, 7, bld. 2, 117312 Moscow, RussiaN.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of the Russian Academy of Sciences, 9 Lavrentiev Avenue, 630090 Novosibirsk, RussiaThe ability of actinobacteria of the genus <i>Rhodococcus</i> to biotransform the monoterpenoid (–)-isopulegol has been established for the first time. <i>R</i>. <i>rhodochrous</i> strain IEGM 1362 was selected as a bacterium capable of metabolizing (–)-isopulegol to form new, previously unknown, 10-hydroxy (<b>2</b>) and 10-carboxy (<b>3</b>) derivatives, which may presumably have antitumor activity and act as respiratory stimulants and cancer prevention agents. In the experiments, optimal conditions were selected to provide the maximum target catalytic activity of rhodococci. Using up-to-date (TEM, AFM-CLSM, and EDX) and traditional (cell size, roughness, and zeta potential measurements) biophysical and microbiological methods, it was shown that (–)-isopulegol and halloysite nanotubes did not negatively affect the bacterial cells. The data obtained expand our knowledge of the biocatalytic potential of rhodococci and their possible involvement in the synthesis of pharmacologically active compounds from plant derivatives.https://www.mdpi.com/1424-8247/15/8/964biotransformationhalloysite nanotubes(–)-isopulegolmonoterpenoid<i>Rhodococcus rhodochrous</i> |
| spellingShingle | Irina B. Ivshina Natalia A. Luchnikova Polina Yu. Maltseva Irina V. Ilyina Konstantin P. Volcho Yurii V. Gatilov Dina V. Korchagina Nadezhda A. Kostrikina Vladimir V. Sorokin Andrey L. Mulyukin Nariman F. Salakhutdinov Biotransformation of (–)-Isopulegol by <i>Rhodococcus rhodochrous</i> Pharmaceuticals biotransformation halloysite nanotubes (–)-isopulegol monoterpenoid <i>Rhodococcus rhodochrous</i> |
| title | Biotransformation of (–)-Isopulegol by <i>Rhodococcus rhodochrous</i> |
| title_full | Biotransformation of (–)-Isopulegol by <i>Rhodococcus rhodochrous</i> |
| title_fullStr | Biotransformation of (–)-Isopulegol by <i>Rhodococcus rhodochrous</i> |
| title_full_unstemmed | Biotransformation of (–)-Isopulegol by <i>Rhodococcus rhodochrous</i> |
| title_short | Biotransformation of (–)-Isopulegol by <i>Rhodococcus rhodochrous</i> |
| title_sort | biotransformation of isopulegol by i rhodococcus rhodochrous i |
| topic | biotransformation halloysite nanotubes (–)-isopulegol monoterpenoid <i>Rhodococcus rhodochrous</i> |
| url | https://www.mdpi.com/1424-8247/15/8/964 |
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