Interference with SPARC inhibits Benzophenone-3 induced ferroptosis in osteoarthritis: Evidence from bioinformatics analyses and biological experimentation
The aim of this study is to conduct a thorough evaluation of the association between Benzophenone-3 (BP-3) exposure and OA, offering critical insights into the underlying mechanisms involved. The National Health and Nutrition Examination Survey (NHANES) database was utilized to investigate the corre...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-04-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324002938 |
| _version_ | 1797243044445028352 |
|---|---|
| author | Yaoyao Nie Houpu Liu Runtao Wu Jiayao Fan Ye Yang Wenxia Zhao Jiapeng Bao Zhenqiang You Fan He Yingjun Li |
| author_facet | Yaoyao Nie Houpu Liu Runtao Wu Jiayao Fan Ye Yang Wenxia Zhao Jiapeng Bao Zhenqiang You Fan He Yingjun Li |
| author_sort | Yaoyao Nie |
| collection | DOAJ |
| description | The aim of this study is to conduct a thorough evaluation of the association between Benzophenone-3 (BP-3) exposure and OA, offering critical insights into the underlying mechanisms involved. The National Health and Nutrition Examination Survey (NHANES) database was utilized to investigate the correlation between BP-3 and osteoarthritis. Proteomic sequencing from clinical sample and the PharmMapper online tool were employed to predict the biological target of BP-3. Cellular molecular assays and transfection studies were performed to verify the prediction from bioinformatics analyses. Through cross-sectional analysis of the NHANES database, we identified BP-3 as a risk factor for OA development. The results of proteomic sequencing showed that Secreted Protein Acidic and Rich in Cysteine (SPARC) was significantly elevated in the area of damage compared to the undamaged area. SPARC was also among the potential biological targets of BP-3 predicted by the online program. Through in vitro cell experiments, we further determined that the toxicological effects of BP-3 may be due to SPARC, which elevates intracellular GPX4 levels, activates the glutathione system, and promotes lipid peroxidation to mitigate ferroptosis. Inhibiting SPARC expression has been shown to reduce inflammation and ferroptosis in OA contexts. This research provides an expansive understanding of BP-3's influence on osteoarthritis development. We have identified SPARC as a potent target for combating chondrocyte ferroptosis in BP-3-associated osteoarthritis. |
| first_indexed | 2024-04-24T18:48:51Z |
| format | Article |
| id | doaj.art-b683b0a01f794e3ea1d28f029f9bab20 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-24T18:48:51Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-b683b0a01f794e3ea1d28f029f9bab202024-03-27T04:51:24ZengElsevierEcotoxicology and Environmental Safety0147-65132024-04-01274116217Interference with SPARC inhibits Benzophenone-3 induced ferroptosis in osteoarthritis: Evidence from bioinformatics analyses and biological experimentationYaoyao Nie0Houpu Liu1Runtao Wu2Jiayao Fan3Ye Yang4Wenxia Zhao5Jiapeng Bao6Zhenqiang You7Fan He8Yingjun Li9Department of Epidemiology and Health Statistics, School of Public Health, Hangzhou Medical College, Hangzhou 310053, ChinaDepartment of Epidemiology and Health Statistics, School of Public Health, Hangzhou Medical College, Hangzhou 310053, ChinaDepartment of Epidemiology and Health Statistics, School of Public Health, Hangzhou Medical College, Hangzhou 310053, ChinaSchool of Public Health and the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China; Department of Epidemiology and Health Statistics, School of Public Health, Hangzhou Medical College, Hangzhou 310053, ChinaDepartment of Epidemiology and Health Statistics, School of Public Health, Hangzhou Medical College, Hangzhou 310053, ChinaDepartment of Epidemiology and Health Statistics, School of Public Health, Hangzhou Medical College, Hangzhou 310053, ChinaDepartment of Orthopaedics, the Second Affiliated Hospital of Zhejiang University, Hangzhou 310058, ChinaDepartment of Food Science and Engineering, School of Public Health, Hangzhou Medical College, Hangzhou 310053, ChinaZhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, ChinaDepartment of Epidemiology and Health Statistics, School of Public Health, Hangzhou Medical College, Hangzhou 310053, China; Correspondence to: Department of Epidemiology and Health Statistics, School of Public Health, Hangzhou Medical College, 481 Binwen Road, Hangzhou 310053, China.The aim of this study is to conduct a thorough evaluation of the association between Benzophenone-3 (BP-3) exposure and OA, offering critical insights into the underlying mechanisms involved. The National Health and Nutrition Examination Survey (NHANES) database was utilized to investigate the correlation between BP-3 and osteoarthritis. Proteomic sequencing from clinical sample and the PharmMapper online tool were employed to predict the biological target of BP-3. Cellular molecular assays and transfection studies were performed to verify the prediction from bioinformatics analyses. Through cross-sectional analysis of the NHANES database, we identified BP-3 as a risk factor for OA development. The results of proteomic sequencing showed that Secreted Protein Acidic and Rich in Cysteine (SPARC) was significantly elevated in the area of damage compared to the undamaged area. SPARC was also among the potential biological targets of BP-3 predicted by the online program. Through in vitro cell experiments, we further determined that the toxicological effects of BP-3 may be due to SPARC, which elevates intracellular GPX4 levels, activates the glutathione system, and promotes lipid peroxidation to mitigate ferroptosis. Inhibiting SPARC expression has been shown to reduce inflammation and ferroptosis in OA contexts. This research provides an expansive understanding of BP-3's influence on osteoarthritis development. We have identified SPARC as a potent target for combating chondrocyte ferroptosis in BP-3-associated osteoarthritis.http://www.sciencedirect.com/science/article/pii/S0147651324002938OsteoarthritisBenzophenone-3SPARCFerroptosis |
| spellingShingle | Yaoyao Nie Houpu Liu Runtao Wu Jiayao Fan Ye Yang Wenxia Zhao Jiapeng Bao Zhenqiang You Fan He Yingjun Li Interference with SPARC inhibits Benzophenone-3 induced ferroptosis in osteoarthritis: Evidence from bioinformatics analyses and biological experimentation Ecotoxicology and Environmental Safety Osteoarthritis Benzophenone-3 SPARC Ferroptosis |
| title | Interference with SPARC inhibits Benzophenone-3 induced ferroptosis in osteoarthritis: Evidence from bioinformatics analyses and biological experimentation |
| title_full | Interference with SPARC inhibits Benzophenone-3 induced ferroptosis in osteoarthritis: Evidence from bioinformatics analyses and biological experimentation |
| title_fullStr | Interference with SPARC inhibits Benzophenone-3 induced ferroptosis in osteoarthritis: Evidence from bioinformatics analyses and biological experimentation |
| title_full_unstemmed | Interference with SPARC inhibits Benzophenone-3 induced ferroptosis in osteoarthritis: Evidence from bioinformatics analyses and biological experimentation |
| title_short | Interference with SPARC inhibits Benzophenone-3 induced ferroptosis in osteoarthritis: Evidence from bioinformatics analyses and biological experimentation |
| title_sort | interference with sparc inhibits benzophenone 3 induced ferroptosis in osteoarthritis evidence from bioinformatics analyses and biological experimentation |
| topic | Osteoarthritis Benzophenone-3 SPARC Ferroptosis |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324002938 |
| work_keys_str_mv | AT yaoyaonie interferencewithsparcinhibitsbenzophenone3inducedferroptosisinosteoarthritisevidencefrombioinformaticsanalysesandbiologicalexperimentation AT houpuliu interferencewithsparcinhibitsbenzophenone3inducedferroptosisinosteoarthritisevidencefrombioinformaticsanalysesandbiologicalexperimentation AT runtaowu interferencewithsparcinhibitsbenzophenone3inducedferroptosisinosteoarthritisevidencefrombioinformaticsanalysesandbiologicalexperimentation AT jiayaofan interferencewithsparcinhibitsbenzophenone3inducedferroptosisinosteoarthritisevidencefrombioinformaticsanalysesandbiologicalexperimentation AT yeyang interferencewithsparcinhibitsbenzophenone3inducedferroptosisinosteoarthritisevidencefrombioinformaticsanalysesandbiologicalexperimentation AT wenxiazhao interferencewithsparcinhibitsbenzophenone3inducedferroptosisinosteoarthritisevidencefrombioinformaticsanalysesandbiologicalexperimentation AT jiapengbao interferencewithsparcinhibitsbenzophenone3inducedferroptosisinosteoarthritisevidencefrombioinformaticsanalysesandbiologicalexperimentation AT zhenqiangyou interferencewithsparcinhibitsbenzophenone3inducedferroptosisinosteoarthritisevidencefrombioinformaticsanalysesandbiologicalexperimentation AT fanhe interferencewithsparcinhibitsbenzophenone3inducedferroptosisinosteoarthritisevidencefrombioinformaticsanalysesandbiologicalexperimentation AT yingjunli interferencewithsparcinhibitsbenzophenone3inducedferroptosisinosteoarthritisevidencefrombioinformaticsanalysesandbiologicalexperimentation |