A Novel Window into Angiogenesis—Intravital Microscopy in the AV-Loop-Model
Due to the limitations of current in vivo experimental designs, our comprehensive knowledge of vascular development and its implications for the development of large-scale engineered tissue constructs is very limited. Therefore, the purpose of this study was to develop unique in vivo imaging chamber...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/2/261 |
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| author | Ravikumar Vaghela Andreas Arkudas Daniel Gage Carolin Körner Stephan von Hörsten Sahar Salehi Raymund E. Horch Maximilian Hessenauer |
| author_facet | Ravikumar Vaghela Andreas Arkudas Daniel Gage Carolin Körner Stephan von Hörsten Sahar Salehi Raymund E. Horch Maximilian Hessenauer |
| author_sort | Ravikumar Vaghela |
| collection | DOAJ |
| description | Due to the limitations of current in vivo experimental designs, our comprehensive knowledge of vascular development and its implications for the development of large-scale engineered tissue constructs is very limited. Therefore, the purpose of this study was to develop unique in vivo imaging chambers that allow the live visualization of cellular processes in the arteriovenous (AV) loop model in rats. We have developed two different types of chambers. Chamber A is installed in the skin using the purse sting fixing method, while chamber B is installed subcutaneously under the skin. Both chambers are filled with modified gelatin hydrogel as a matrix. Intravital microscopy (IVM) was performed after the injection of fluorescein isothiocyanate (FITC)-labeled dextran and rhodamine 6G dye. The AV loop was functional for two weeks in chamber A and allowed visualization of the leukocyte trafficking. In chamber B, microvascular development in the AV loop could be examined for 21 days. Quantification of the microvascular outgrowth was performed using Fiji-ImageJ. Overall, by combining these two IVM chambers, we can comprehensively understand vascular development in the AV loop tissue engineering model¯. |
| first_indexed | 2024-03-09T13:12:52Z |
| format | Article |
| id | doaj.art-b68920ccde2344668a4dcd5c3d968046 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T13:12:52Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-b68920ccde2344668a4dcd5c3d9680462023-11-30T21:39:57ZengMDPI AGCells2073-44092023-01-0112226110.3390/cells12020261A Novel Window into Angiogenesis—Intravital Microscopy in the AV-Loop-ModelRavikumar Vaghela0Andreas Arkudas1Daniel Gage2Carolin Körner3Stephan von Hörsten4Sahar Salehi5Raymund E. Horch6Maximilian Hessenauer7Department of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), 91054 Erlangen, GermanyDepartment of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), 91054 Erlangen, GermanyDepartment of Materials Science and Engineering for Metals, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), 91058 Erlangen, GermanyDepartment of Materials Science and Engineering for Metals, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), 91058 Erlangen, GermanyDepartment of Experimental Therapy, University Hospital of Erlangen and Preclinical Experimental Animal Center, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), 91054 Erlangen, GermanyDepartment of Biomaterials, University of Bayreuth, 95447 Bayreuth, GermanyDepartment of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), 91054 Erlangen, GermanyDepartment of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), 91054 Erlangen, GermanyDue to the limitations of current in vivo experimental designs, our comprehensive knowledge of vascular development and its implications for the development of large-scale engineered tissue constructs is very limited. Therefore, the purpose of this study was to develop unique in vivo imaging chambers that allow the live visualization of cellular processes in the arteriovenous (AV) loop model in rats. We have developed two different types of chambers. Chamber A is installed in the skin using the purse sting fixing method, while chamber B is installed subcutaneously under the skin. Both chambers are filled with modified gelatin hydrogel as a matrix. Intravital microscopy (IVM) was performed after the injection of fluorescein isothiocyanate (FITC)-labeled dextran and rhodamine 6G dye. The AV loop was functional for two weeks in chamber A and allowed visualization of the leukocyte trafficking. In chamber B, microvascular development in the AV loop could be examined for 21 days. Quantification of the microvascular outgrowth was performed using Fiji-ImageJ. Overall, by combining these two IVM chambers, we can comprehensively understand vascular development in the AV loop tissue engineering model¯.https://www.mdpi.com/2073-4409/12/2/261intravital microscopyarteriovenous looptissue engineeringGelMA |
| spellingShingle | Ravikumar Vaghela Andreas Arkudas Daniel Gage Carolin Körner Stephan von Hörsten Sahar Salehi Raymund E. Horch Maximilian Hessenauer A Novel Window into Angiogenesis—Intravital Microscopy in the AV-Loop-Model Cells intravital microscopy arteriovenous loop tissue engineering GelMA |
| title | A Novel Window into Angiogenesis—Intravital Microscopy in the AV-Loop-Model |
| title_full | A Novel Window into Angiogenesis—Intravital Microscopy in the AV-Loop-Model |
| title_fullStr | A Novel Window into Angiogenesis—Intravital Microscopy in the AV-Loop-Model |
| title_full_unstemmed | A Novel Window into Angiogenesis—Intravital Microscopy in the AV-Loop-Model |
| title_short | A Novel Window into Angiogenesis—Intravital Microscopy in the AV-Loop-Model |
| title_sort | novel window into angiogenesis intravital microscopy in the av loop model |
| topic | intravital microscopy arteriovenous loop tissue engineering GelMA |
| url | https://www.mdpi.com/2073-4409/12/2/261 |
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