Characterization of Lipids in Saliva, Tears and Minor Salivary Glands of Sjögren’s Syndrome Patients Using an HPLC/MS-Based Approach
The diagnostic work-up of primary Sjögren’s syndrome (pSS) includes quantifying saliva and tear production, evaluation of autoantibodies in serum and histopathological analysis of minor salivary glands. Thus, the potential for further utilizing these fluids and tissues in the quest to find better di...
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| Format: | Article |
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/16/8997 |
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| author | Fredrik Fineide Xiangjun Chen Thomas Bjellaas Valeria Vitelli Tor Paaske Utheim Janicke Liaaen Jensen Hilde Kanli Galtung |
| author_facet | Fredrik Fineide Xiangjun Chen Thomas Bjellaas Valeria Vitelli Tor Paaske Utheim Janicke Liaaen Jensen Hilde Kanli Galtung |
| author_sort | Fredrik Fineide |
| collection | DOAJ |
| description | The diagnostic work-up of primary Sjögren’s syndrome (pSS) includes quantifying saliva and tear production, evaluation of autoantibodies in serum and histopathological analysis of minor salivary glands. Thus, the potential for further utilizing these fluids and tissues in the quest to find better diagnostic and therapeutic tools should be fully explored. Ten samples of saliva and tears from female patients diagnosed with pSS and ten samples of saliva and tears from healthy females were included for lipidomic analysis of tears and whole saliva using high-performance liquid chromatography coupled to time-of-flight mass spectrometry. In addition, lipidomic analysis was performed on minor salivary gland biopsies from three pSS and three non-SS females. We found significant differences in the lipidomic profiles of saliva and tears in pSS patients compared to healthy controls. Moreover, there were differences in individual lipid species in stimulated saliva that were comparable to those of glandular biopsies, representing an intriguing avenue for further research. We believe a comprehensive elucidation of the changes in lipid composition in saliva, tears and minor salivary glands in pSS patients may be the key to detecting pSS-related dry mouth and dry eyes at an early stage. The identified differences may illuminate the path towards future innovative diagnostic methodologies and treatment modalities for alleviating pSS-related sicca symptoms. |
| first_indexed | 2024-03-10T08:44:12Z |
| format | Article |
| id | doaj.art-b68b8eefd7b74a789075371afd94b21e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:44:12Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b68b8eefd7b74a789075371afd94b21e2023-11-22T08:03:50ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012216899710.3390/ijms22168997Characterization of Lipids in Saliva, Tears and Minor Salivary Glands of Sjögren’s Syndrome Patients Using an HPLC/MS-Based ApproachFredrik Fineide0Xiangjun Chen1Thomas Bjellaas2Valeria Vitelli3Tor Paaske Utheim4Janicke Liaaen Jensen5Hilde Kanli Galtung6Department of Plastic and Reconstructive Surgery, Oslo University Hospital, 1171 Oslo, NorwayDepartment of Oral Surgery and Oral Medicine, Faculty of Dentistry, University of Oslo, 0317 Oslo, NorwayVITAS Analytical Services, 0349 Oslo, NorwayDepartment of Biostatistics, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, 0316 Oslo, NorwayDepartment of Plastic and Reconstructive Surgery, Oslo University Hospital, 1171 Oslo, NorwayDepartment of Oral Surgery and Oral Medicine, Faculty of Dentistry, University of Oslo, 0317 Oslo, NorwayDepartment of Oral Biology, Faculty of Dentistry, University of Oslo, 0316 Oslo, NorwayThe diagnostic work-up of primary Sjögren’s syndrome (pSS) includes quantifying saliva and tear production, evaluation of autoantibodies in serum and histopathological analysis of minor salivary glands. Thus, the potential for further utilizing these fluids and tissues in the quest to find better diagnostic and therapeutic tools should be fully explored. Ten samples of saliva and tears from female patients diagnosed with pSS and ten samples of saliva and tears from healthy females were included for lipidomic analysis of tears and whole saliva using high-performance liquid chromatography coupled to time-of-flight mass spectrometry. In addition, lipidomic analysis was performed on minor salivary gland biopsies from three pSS and three non-SS females. We found significant differences in the lipidomic profiles of saliva and tears in pSS patients compared to healthy controls. Moreover, there were differences in individual lipid species in stimulated saliva that were comparable to those of glandular biopsies, representing an intriguing avenue for further research. We believe a comprehensive elucidation of the changes in lipid composition in saliva, tears and minor salivary glands in pSS patients may be the key to detecting pSS-related dry mouth and dry eyes at an early stage. The identified differences may illuminate the path towards future innovative diagnostic methodologies and treatment modalities for alleviating pSS-related sicca symptoms.https://www.mdpi.com/1422-0067/22/16/8997dry eye diseaseeye/retinainflammationlipidomicsliquid chromatographymass spectrometry |
| spellingShingle | Fredrik Fineide Xiangjun Chen Thomas Bjellaas Valeria Vitelli Tor Paaske Utheim Janicke Liaaen Jensen Hilde Kanli Galtung Characterization of Lipids in Saliva, Tears and Minor Salivary Glands of Sjögren’s Syndrome Patients Using an HPLC/MS-Based Approach International Journal of Molecular Sciences dry eye disease eye/retina inflammation lipidomics liquid chromatography mass spectrometry |
| title | Characterization of Lipids in Saliva, Tears and Minor Salivary Glands of Sjögren’s Syndrome Patients Using an HPLC/MS-Based Approach |
| title_full | Characterization of Lipids in Saliva, Tears and Minor Salivary Glands of Sjögren’s Syndrome Patients Using an HPLC/MS-Based Approach |
| title_fullStr | Characterization of Lipids in Saliva, Tears and Minor Salivary Glands of Sjögren’s Syndrome Patients Using an HPLC/MS-Based Approach |
| title_full_unstemmed | Characterization of Lipids in Saliva, Tears and Minor Salivary Glands of Sjögren’s Syndrome Patients Using an HPLC/MS-Based Approach |
| title_short | Characterization of Lipids in Saliva, Tears and Minor Salivary Glands of Sjögren’s Syndrome Patients Using an HPLC/MS-Based Approach |
| title_sort | characterization of lipids in saliva tears and minor salivary glands of sjogren s syndrome patients using an hplc ms based approach |
| topic | dry eye disease eye/retina inflammation lipidomics liquid chromatography mass spectrometry |
| url | https://www.mdpi.com/1422-0067/22/16/8997 |
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