Identification of polycomb repressive complex 1 and 2 core components in hexaploid bread wheat
Abstract Background Polycomb repressive complexes 1 and 2 play important roles in epigenetic gene regulation by posttranslationally modifying specific histone residues. Polycomb repressive complex 2 is responsible for the trimethylation of lysine 27 on histone H3; Polycomb repressive complex 1 catal...
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BMC
2020-10-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-020-02384-6 |
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| author | Beáta Strejčková Radim Čegan Ales Pecinka Zbyněk Milec Jan Šafář |
| author_facet | Beáta Strejčková Radim Čegan Ales Pecinka Zbyněk Milec Jan Šafář |
| author_sort | Beáta Strejčková |
| collection | DOAJ |
| description | Abstract Background Polycomb repressive complexes 1 and 2 play important roles in epigenetic gene regulation by posttranslationally modifying specific histone residues. Polycomb repressive complex 2 is responsible for the trimethylation of lysine 27 on histone H3; Polycomb repressive complex 1 catalyzes the monoubiquitination of histone H2A at lysine 119. Both complexes have been thoroughly studied in Arabidopsis, but the evolution of polycomb group gene families in monocots, particularly those with complex allopolyploid origins, is unknown. Results Here, we present the in silico identification of the Polycomb repressive complex 1 and 2 (PRC2, PRC1) subunits in allohexaploid bread wheat, the reconstruction of their evolutionary history and a transcriptional analysis over a series of 33 developmental stages. We identified four main subunits of PRC2 [E(z), Su(z), FIE and MSI] and three main subunits of PRC1 (Pc, Psc and Sce) and determined their chromosomal locations. We found that most of the genes coding for subunit proteins are present as paralogs in bread wheat. Using bread wheat RNA-seq data from different tissues and developmental stages throughout plant ontogenesis revealed variable transcriptional activity for individual paralogs. Phylogenetic analysis showed a high level of protein conservation among temperate cereals. Conclusions The identification and chromosomal location of the Polycomb repressive complex 1 and 2 core components in bread wheat may enable a deeper understanding of developmental processes, including vernalization, in commonly grown winter wheat. |
| first_indexed | 2024-12-14T09:51:26Z |
| format | Article |
| id | doaj.art-b68be5490df04bd1becd4c7411fca91c |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-14T09:51:26Z |
| publishDate | 2020-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-b68be5490df04bd1becd4c7411fca91c2022-12-21T23:07:31ZengBMCBMC Plant Biology1471-22292020-10-0120S111310.1186/s12870-020-02384-6Identification of polycomb repressive complex 1 and 2 core components in hexaploid bread wheatBeáta Strejčková0Radim Čegan1Ales Pecinka2Zbyněk Milec3Jan Šafář4Institute of Experimental Botany, Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural ResearchInstitute of Experimental Botany, Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural ResearchInstitute of Experimental Botany, Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural ResearchInstitute of Experimental Botany, Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural ResearchInstitute of Experimental Botany, Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural ResearchAbstract Background Polycomb repressive complexes 1 and 2 play important roles in epigenetic gene regulation by posttranslationally modifying specific histone residues. Polycomb repressive complex 2 is responsible for the trimethylation of lysine 27 on histone H3; Polycomb repressive complex 1 catalyzes the monoubiquitination of histone H2A at lysine 119. Both complexes have been thoroughly studied in Arabidopsis, but the evolution of polycomb group gene families in monocots, particularly those with complex allopolyploid origins, is unknown. Results Here, we present the in silico identification of the Polycomb repressive complex 1 and 2 (PRC2, PRC1) subunits in allohexaploid bread wheat, the reconstruction of their evolutionary history and a transcriptional analysis over a series of 33 developmental stages. We identified four main subunits of PRC2 [E(z), Su(z), FIE and MSI] and three main subunits of PRC1 (Pc, Psc and Sce) and determined their chromosomal locations. We found that most of the genes coding for subunit proteins are present as paralogs in bread wheat. Using bread wheat RNA-seq data from different tissues and developmental stages throughout plant ontogenesis revealed variable transcriptional activity for individual paralogs. Phylogenetic analysis showed a high level of protein conservation among temperate cereals. Conclusions The identification and chromosomal location of the Polycomb repressive complex 1 and 2 core components in bread wheat may enable a deeper understanding of developmental processes, including vernalization, in commonly grown winter wheat.http://link.springer.com/article/10.1186/s12870-020-02384-6Polycomb repressive complexEpigeneticsPRC2WheatHistone methylation |
| spellingShingle | Beáta Strejčková Radim Čegan Ales Pecinka Zbyněk Milec Jan Šafář Identification of polycomb repressive complex 1 and 2 core components in hexaploid bread wheat BMC Plant Biology Polycomb repressive complex Epigenetics PRC2 Wheat Histone methylation |
| title | Identification of polycomb repressive complex 1 and 2 core components in hexaploid bread wheat |
| title_full | Identification of polycomb repressive complex 1 and 2 core components in hexaploid bread wheat |
| title_fullStr | Identification of polycomb repressive complex 1 and 2 core components in hexaploid bread wheat |
| title_full_unstemmed | Identification of polycomb repressive complex 1 and 2 core components in hexaploid bread wheat |
| title_short | Identification of polycomb repressive complex 1 and 2 core components in hexaploid bread wheat |
| title_sort | identification of polycomb repressive complex 1 and 2 core components in hexaploid bread wheat |
| topic | Polycomb repressive complex Epigenetics PRC2 Wheat Histone methylation |
| url | http://link.springer.com/article/10.1186/s12870-020-02384-6 |
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