Star Polycation Mediated dsRNA Improves the Efficiency of RNA Interference in <i>Phytoseiulus persimilis</i>
RNA interference (RNAi) is one of the most widely used techniques to study gene functions. There is still a lack of RNAi techniques that can be applied in Phytoseiidae conveniently and efficiently. Star Polycation is a new nanomaterial commonly used as a carrier of dsRNA in RNAi. Five genes of <i...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/21/3809 |
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| author | Zhenhui Wang Mingxia Li Ziyi Kong Endong Wang Bo Zhang Jiale Lv Xuenong Xu |
| author_facet | Zhenhui Wang Mingxia Li Ziyi Kong Endong Wang Bo Zhang Jiale Lv Xuenong Xu |
| author_sort | Zhenhui Wang |
| collection | DOAJ |
| description | RNA interference (RNAi) is one of the most widely used techniques to study gene functions. There is still a lack of RNAi techniques that can be applied in Phytoseiidae conveniently and efficiently. Star Polycation is a new nanomaterial commonly used as a carrier of dsRNA in RNAi. Five genes of <i>P. persimilis</i> (<i>PpATPb</i>, <i>PpATPd</i>, <i>PpRpL11</i>, <i>PpRpS2</i>, and <i>Pptra-2</i>) were selected to verify whether SPc promotes the delivery of dsRNA into <i>P. persimilis</i> through soaking. When each of the five genes were interfered using SPc-mediated dsRNA, the total number of success offspring produced per female in six days decreased by ca. 92%, 92%, 91%, 96%, and 64%. When <i>PpATPb</i>, <i>PpATPd</i>, <i>PpRpL11</i>, or <i>PpRpS2</i> was interfered, both the fecundity and egg hatching rate decreased. In contrast, when <i>Pptra-2</i> was interfered, reduction in the reproductive capability was mainly the result of the decreased egg hatching rate. Correspondingly, when the target gene was interfered, <i>P. persimilis</i> expression of <i>PpRpL11</i> reduced by 63.95%, while that of the other four genes reduced by at least 80%. Our studies showed that nanomaterials, such as SPc, have the potential to be used in RNA interference of phytoseiid mites. |
| first_indexed | 2024-03-09T18:47:17Z |
| format | Article |
| id | doaj.art-b68bec6e161d4f39b2cf0cfb214d7d05 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T18:47:17Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-b68bec6e161d4f39b2cf0cfb214d7d052023-11-24T06:09:31ZengMDPI AGNanomaterials2079-49912022-10-011221380910.3390/nano12213809Star Polycation Mediated dsRNA Improves the Efficiency of RNA Interference in <i>Phytoseiulus persimilis</i>Zhenhui Wang0Mingxia Li1Ziyi Kong2Endong Wang3Bo Zhang4Jiale Lv5Xuenong Xu6Lab of Predatory Mites, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (IPPCAAS), No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, ChinaLab of Predatory Mites, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (IPPCAAS), No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, ChinaLab of Predatory Mites, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (IPPCAAS), No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, ChinaLab of Predatory Mites, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (IPPCAAS), No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, ChinaLab of Predatory Mites, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (IPPCAAS), No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, ChinaLab of Predatory Mites, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (IPPCAAS), No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, ChinaLab of Predatory Mites, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (IPPCAAS), No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, ChinaRNA interference (RNAi) is one of the most widely used techniques to study gene functions. There is still a lack of RNAi techniques that can be applied in Phytoseiidae conveniently and efficiently. Star Polycation is a new nanomaterial commonly used as a carrier of dsRNA in RNAi. Five genes of <i>P. persimilis</i> (<i>PpATPb</i>, <i>PpATPd</i>, <i>PpRpL11</i>, <i>PpRpS2</i>, and <i>Pptra-2</i>) were selected to verify whether SPc promotes the delivery of dsRNA into <i>P. persimilis</i> through soaking. When each of the five genes were interfered using SPc-mediated dsRNA, the total number of success offspring produced per female in six days decreased by ca. 92%, 92%, 91%, 96%, and 64%. When <i>PpATPb</i>, <i>PpATPd</i>, <i>PpRpL11</i>, or <i>PpRpS2</i> was interfered, both the fecundity and egg hatching rate decreased. In contrast, when <i>Pptra-2</i> was interfered, reduction in the reproductive capability was mainly the result of the decreased egg hatching rate. Correspondingly, when the target gene was interfered, <i>P. persimilis</i> expression of <i>PpRpL11</i> reduced by 63.95%, while that of the other four genes reduced by at least 80%. Our studies showed that nanomaterials, such as SPc, have the potential to be used in RNA interference of phytoseiid mites.https://www.mdpi.com/2079-4991/12/21/3809Phytoseiidaenanomaterialsoakingreproduction |
| spellingShingle | Zhenhui Wang Mingxia Li Ziyi Kong Endong Wang Bo Zhang Jiale Lv Xuenong Xu Star Polycation Mediated dsRNA Improves the Efficiency of RNA Interference in <i>Phytoseiulus persimilis</i> Nanomaterials Phytoseiidae nanomaterial soaking reproduction |
| title | Star Polycation Mediated dsRNA Improves the Efficiency of RNA Interference in <i>Phytoseiulus persimilis</i> |
| title_full | Star Polycation Mediated dsRNA Improves the Efficiency of RNA Interference in <i>Phytoseiulus persimilis</i> |
| title_fullStr | Star Polycation Mediated dsRNA Improves the Efficiency of RNA Interference in <i>Phytoseiulus persimilis</i> |
| title_full_unstemmed | Star Polycation Mediated dsRNA Improves the Efficiency of RNA Interference in <i>Phytoseiulus persimilis</i> |
| title_short | Star Polycation Mediated dsRNA Improves the Efficiency of RNA Interference in <i>Phytoseiulus persimilis</i> |
| title_sort | star polycation mediated dsrna improves the efficiency of rna interference in i phytoseiulus persimilis i |
| topic | Phytoseiidae nanomaterial soaking reproduction |
| url | https://www.mdpi.com/2079-4991/12/21/3809 |
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