Genome-wide identification and characterization of toll-like receptor 5 (TLR5) in fishes
Toll-like receptors 5 (TLR5), a member of the toll-like receptors (TLRs) family, is a class of pattern recognition receptors (PRRs) that recognize pathogen-associated molecular patterns (PAMPs). It responds to vertebrate recognition of bacterial flagellin and participates in innate immune responses....
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.1083578/full |
| _version_ | 1797959981764444160 |
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| author | Kai Zhang Kai Zhang Ming Chen Ming Chen Haobin He Hongyan Kou Hongyan Kou Li Lin Li Lin Rishen Liang Rishen Liang |
| author_facet | Kai Zhang Kai Zhang Ming Chen Ming Chen Haobin He Hongyan Kou Hongyan Kou Li Lin Li Lin Rishen Liang Rishen Liang |
| author_sort | Kai Zhang |
| collection | DOAJ |
| description | Toll-like receptors 5 (TLR5), a member of the toll-like receptors (TLRs) family, is a class of pattern recognition receptors (PRRs) that recognize pathogen-associated molecular patterns (PAMPs). It responds to vertebrate recognition of bacterial flagellin and participates in innate immune responses. However, genome-wide identification and characterization of TLR5 in fishes have not been investigated. Here, three TLR5M isotypes (TLR5Ma, TLR5Mb1, and TLR5Mb2) and a TLR5S are all extracted from fish genomes on the basis of phylogenetic and synteny analyses. We confirmed that the non-teleost fishes have one TLR5M gene, as well as additional TLR5 genes (TLR5M and TLR5S) in teleost fishes. In addition, some special teleost fishes possess two to three TLR5 genes, which have undergone the fourth whole-genome duplication (WGD). According to our results, we inferred that the diversity of TLR5 genes in fishes seems to be the result of combinations of WGD and gene loss. Furthermore, TLR5 isoforms displayed differences at the flagellin interaction sites and viral binding sites, and showed lineage-specific, which indicated that TLR5 duplicates may generate functional divergence. Bacterial experiments also supported the idea that CiTLR5Ma and CiTLR5Mb are subfunctionalized to sense bacterial flagellin. In summary, our present comparative genomic survey will benefit for further functional investigations of TLR5 genes in fish. |
| first_indexed | 2024-04-11T00:39:30Z |
| format | Article |
| id | doaj.art-b68c04a1a60c4069b65731abdee7e22a |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-11T00:39:30Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-b68c04a1a60c4069b65731abdee7e22a2023-01-06T11:31:06ZengFrontiers Media S.A.Frontiers in Genetics1664-80212023-01-011310.3389/fgene.2022.10835781083578Genome-wide identification and characterization of toll-like receptor 5 (TLR5) in fishesKai Zhang0Kai Zhang1Ming Chen2Ming Chen3Haobin He4Hongyan Kou5Hongyan Kou6Li Lin7Li Lin8Rishen Liang9Rishen Liang10College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou, ChinaCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou, ChinaCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou, ChinaCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou, ChinaCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou, ChinaToll-like receptors 5 (TLR5), a member of the toll-like receptors (TLRs) family, is a class of pattern recognition receptors (PRRs) that recognize pathogen-associated molecular patterns (PAMPs). It responds to vertebrate recognition of bacterial flagellin and participates in innate immune responses. However, genome-wide identification and characterization of TLR5 in fishes have not been investigated. Here, three TLR5M isotypes (TLR5Ma, TLR5Mb1, and TLR5Mb2) and a TLR5S are all extracted from fish genomes on the basis of phylogenetic and synteny analyses. We confirmed that the non-teleost fishes have one TLR5M gene, as well as additional TLR5 genes (TLR5M and TLR5S) in teleost fishes. In addition, some special teleost fishes possess two to three TLR5 genes, which have undergone the fourth whole-genome duplication (WGD). According to our results, we inferred that the diversity of TLR5 genes in fishes seems to be the result of combinations of WGD and gene loss. Furthermore, TLR5 isoforms displayed differences at the flagellin interaction sites and viral binding sites, and showed lineage-specific, which indicated that TLR5 duplicates may generate functional divergence. Bacterial experiments also supported the idea that CiTLR5Ma and CiTLR5Mb are subfunctionalized to sense bacterial flagellin. In summary, our present comparative genomic survey will benefit for further functional investigations of TLR5 genes in fish.https://www.frontiersin.org/articles/10.3389/fgene.2022.1083578/fullTLR5fishwhole-genome duplicationsgene lossadaptive evolution |
| spellingShingle | Kai Zhang Kai Zhang Ming Chen Ming Chen Haobin He Hongyan Kou Hongyan Kou Li Lin Li Lin Rishen Liang Rishen Liang Genome-wide identification and characterization of toll-like receptor 5 (TLR5) in fishes Frontiers in Genetics TLR5 fish whole-genome duplications gene loss adaptive evolution |
| title | Genome-wide identification and characterization of toll-like receptor 5 (TLR5) in fishes |
| title_full | Genome-wide identification and characterization of toll-like receptor 5 (TLR5) in fishes |
| title_fullStr | Genome-wide identification and characterization of toll-like receptor 5 (TLR5) in fishes |
| title_full_unstemmed | Genome-wide identification and characterization of toll-like receptor 5 (TLR5) in fishes |
| title_short | Genome-wide identification and characterization of toll-like receptor 5 (TLR5) in fishes |
| title_sort | genome wide identification and characterization of toll like receptor 5 tlr5 in fishes |
| topic | TLR5 fish whole-genome duplications gene loss adaptive evolution |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.1083578/full |
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