Antimicrobial Activity of Cobalt (II)-Citrate against Common Foodborne Pathogens and Its Potential for Incorporation into Food Packaging Material
Novel antimicrobial compounds can be added to foods directly or incorporated into packaging materials in order to improve food safety and shelf life. One such potential antimicrobial compound is the bioinorganic complex Co(II)-citrate (NH<sub>4</sub>)<sub>4</sub>[Co(C<sub&...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/21/10855 |
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| author | Jonathan Rhoades Vilelmini Katsouda Sevasti Matsia Konstantinos Rogkotis Stella Taousani Nonna Kiriazidi Athanasios Salifoglou Eleni Likotrafiti |
| author_facet | Jonathan Rhoades Vilelmini Katsouda Sevasti Matsia Konstantinos Rogkotis Stella Taousani Nonna Kiriazidi Athanasios Salifoglou Eleni Likotrafiti |
| author_sort | Jonathan Rhoades |
| collection | DOAJ |
| description | Novel antimicrobial compounds can be added to foods directly or incorporated into packaging materials in order to improve food safety and shelf life. One such potential antimicrobial compound is the bioinorganic complex Co(II)-citrate (NH<sub>4</sub>)<sub>4</sub>[Co(C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>)<sub>2</sub>] (Co-cit). Its antimicrobial activity against <i>Listeria monocytogenes</i>, <i>Staphylococcus aureus</i>, <i>Escherichia coli,</i> and <i>Pseudomonas aeruginosa</i> was investigated in solution, both alone and in combination with existing preservatives. The antimicrobial activity of poly(lactic acid) films (PLA) with incorporated Co-cit (23.3% <i>w</i>/<i>w</i>) against <i>L. monocytogenes</i> was determined using culture medium and model foods (slices of turkey ham and smoked salmon). In nutrient broth, without preservatives, all four bacteria were significantly (<i>p</i> < 0.05) inhibited by 0.5 mM Co-cit, and <i>L. monocytogenes</i> and <i>P. aeruginosa</i> were the most sensitive. The addition of preservatives to the broth increased the antimicrobial activity of Co-cit in many cases, but not in a way that was consistent between the different bacteria investigated and was likely due to additional physiological stress exerted on the cells rather than any effect on the activity of the Co-cit itself. PLA films with Co-cit were bacteriostatic against <i>L. monocytogenes</i> on artificial media and on foods. However, in the latter case, the PLA film itself was the main contributor to the inhibition. Composite PLA-(Co-cit) films exhibited antimicrobial activity against foodborne bacteria and have potential application as active packaging materials to enhance food safety. |
| first_indexed | 2024-03-09T19:18:24Z |
| format | Article |
| id | doaj.art-b68c6200106142e681e8980dc3ae0171 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T19:18:24Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-b68c6200106142e681e8980dc3ae01712023-11-24T03:33:52ZengMDPI AGApplied Sciences2076-34172022-10-0112211085510.3390/app122110855Antimicrobial Activity of Cobalt (II)-Citrate against Common Foodborne Pathogens and Its Potential for Incorporation into Food Packaging MaterialJonathan Rhoades0Vilelmini Katsouda1Sevasti Matsia2Konstantinos Rogkotis3Stella Taousani4Nonna Kiriazidi5Athanasios Salifoglou6Eleni Likotrafiti7Laboratory of Food Microbiology, Department of Food Science and Technology, International Hellenic University, 57400 Thessaloniki, GreeceLaboratory of Food Microbiology, Department of Food Science and Technology, International Hellenic University, 57400 Thessaloniki, GreeceLaboratory of Inorganic Chemistry and Advanced Materials, School of Chemical Engineering, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Inorganic Chemistry and Advanced Materials, School of Chemical Engineering, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Food Microbiology, Department of Food Science and Technology, International Hellenic University, 57400 Thessaloniki, GreeceLaboratory of Food Microbiology, Department of Food Science and Technology, International Hellenic University, 57400 Thessaloniki, GreeceLaboratory of Inorganic Chemistry and Advanced Materials, School of Chemical Engineering, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Food Microbiology, Department of Food Science and Technology, International Hellenic University, 57400 Thessaloniki, GreeceNovel antimicrobial compounds can be added to foods directly or incorporated into packaging materials in order to improve food safety and shelf life. One such potential antimicrobial compound is the bioinorganic complex Co(II)-citrate (NH<sub>4</sub>)<sub>4</sub>[Co(C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>)<sub>2</sub>] (Co-cit). Its antimicrobial activity against <i>Listeria monocytogenes</i>, <i>Staphylococcus aureus</i>, <i>Escherichia coli,</i> and <i>Pseudomonas aeruginosa</i> was investigated in solution, both alone and in combination with existing preservatives. The antimicrobial activity of poly(lactic acid) films (PLA) with incorporated Co-cit (23.3% <i>w</i>/<i>w</i>) against <i>L. monocytogenes</i> was determined using culture medium and model foods (slices of turkey ham and smoked salmon). In nutrient broth, without preservatives, all four bacteria were significantly (<i>p</i> < 0.05) inhibited by 0.5 mM Co-cit, and <i>L. monocytogenes</i> and <i>P. aeruginosa</i> were the most sensitive. The addition of preservatives to the broth increased the antimicrobial activity of Co-cit in many cases, but not in a way that was consistent between the different bacteria investigated and was likely due to additional physiological stress exerted on the cells rather than any effect on the activity of the Co-cit itself. PLA films with Co-cit were bacteriostatic against <i>L. monocytogenes</i> on artificial media and on foods. However, in the latter case, the PLA film itself was the main contributor to the inhibition. Composite PLA-(Co-cit) films exhibited antimicrobial activity against foodborne bacteria and have potential application as active packaging materials to enhance food safety.https://www.mdpi.com/2076-3417/12/21/10855shelf-life extension<i>Listeria monocytogenes</i>active packagingfood surfaces |
| spellingShingle | Jonathan Rhoades Vilelmini Katsouda Sevasti Matsia Konstantinos Rogkotis Stella Taousani Nonna Kiriazidi Athanasios Salifoglou Eleni Likotrafiti Antimicrobial Activity of Cobalt (II)-Citrate against Common Foodborne Pathogens and Its Potential for Incorporation into Food Packaging Material Applied Sciences shelf-life extension <i>Listeria monocytogenes</i> active packaging food surfaces |
| title | Antimicrobial Activity of Cobalt (II)-Citrate against Common Foodborne Pathogens and Its Potential for Incorporation into Food Packaging Material |
| title_full | Antimicrobial Activity of Cobalt (II)-Citrate against Common Foodborne Pathogens and Its Potential for Incorporation into Food Packaging Material |
| title_fullStr | Antimicrobial Activity of Cobalt (II)-Citrate against Common Foodborne Pathogens and Its Potential for Incorporation into Food Packaging Material |
| title_full_unstemmed | Antimicrobial Activity of Cobalt (II)-Citrate against Common Foodborne Pathogens and Its Potential for Incorporation into Food Packaging Material |
| title_short | Antimicrobial Activity of Cobalt (II)-Citrate against Common Foodborne Pathogens and Its Potential for Incorporation into Food Packaging Material |
| title_sort | antimicrobial activity of cobalt ii citrate against common foodborne pathogens and its potential for incorporation into food packaging material |
| topic | shelf-life extension <i>Listeria monocytogenes</i> active packaging food surfaces |
| url | https://www.mdpi.com/2076-3417/12/21/10855 |
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