RNA-Seq transcriptome reveals different molecular responses during human and mouse oocyte maturation and fertilization
Abstract Background Female infertility is a worldwide concern and the etiology of infertility has not been thoroughly demonstrated. Although the mouse is a good model system to perform functional studies, the differences between mouse and human also need to be considered. The objective of this study...
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| Format: | Article |
| Language: | English |
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BMC
2020-07-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12864-020-06885-4 |
| _version_ | 1818215565623296000 |
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| author | Zheng-Hui Zhao Tie-Gang Meng Ang Li Heide Schatten Zhen-Bo Wang Qing-Yuan Sun |
| author_facet | Zheng-Hui Zhao Tie-Gang Meng Ang Li Heide Schatten Zhen-Bo Wang Qing-Yuan Sun |
| author_sort | Zheng-Hui Zhao |
| collection | DOAJ |
| description | Abstract Background Female infertility is a worldwide concern and the etiology of infertility has not been thoroughly demonstrated. Although the mouse is a good model system to perform functional studies, the differences between mouse and human also need to be considered. The objective of this study is to elucidate the different molecular mechanisms underlying oocyte maturation and fertilization between human and mouse. Results A comparative transcriptome analysis was performed to identify the differentially expressed genes and associated biological processes between human and mouse oocytes. In total, 8513 common genes, as well as 15,165 and 6126 uniquely expressed genes were detected in human and mouse MII oocytes, respectively. Additionally, the ratios of non-homologous genes in human and mouse MII oocytes were 37 and 8%, respectively. Functional categorization analysis of the human MII non-homologous genes revealed that cAMP-mediated signaling, sister chromatid cohesin, and cell recognition were the major enriched biological processes. Interestingly, we couldn’t detect any GO categories in mouse non-homologous genes. Conclusions This study demonstrates that human and mouse oocytes exhibit significant differences in gene expression profiles during oocyte maturation, which probably deciphers the differential molecular responses to oocyte maturation and fertilization. The significant differences between human and mouse oocytes limit the generalizations from mouse to human oocyte maturation. Knowledge about the limitations of animal models is crucial when exploring a complex process such as human oocyte maturation and fertilization. |
| first_indexed | 2024-12-12T06:38:06Z |
| format | Article |
| id | doaj.art-b68f655f518b4d97aee8ffa57b4084c3 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-12T06:38:06Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-b68f655f518b4d97aee8ffa57b4084c32022-12-22T00:34:25ZengBMCBMC Genomics1471-21642020-07-0121111110.1186/s12864-020-06885-4RNA-Seq transcriptome reveals different molecular responses during human and mouse oocyte maturation and fertilizationZheng-Hui Zhao0Tie-Gang Meng1Ang Li2Heide Schatten3Zhen-Bo Wang4Qing-Yuan Sun5State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of SciencesState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of SciencesState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of SciencesDepartment of Veterinary Pathobiology, University of MissouriState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of SciencesState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of SciencesAbstract Background Female infertility is a worldwide concern and the etiology of infertility has not been thoroughly demonstrated. Although the mouse is a good model system to perform functional studies, the differences between mouse and human also need to be considered. The objective of this study is to elucidate the different molecular mechanisms underlying oocyte maturation and fertilization between human and mouse. Results A comparative transcriptome analysis was performed to identify the differentially expressed genes and associated biological processes between human and mouse oocytes. In total, 8513 common genes, as well as 15,165 and 6126 uniquely expressed genes were detected in human and mouse MII oocytes, respectively. Additionally, the ratios of non-homologous genes in human and mouse MII oocytes were 37 and 8%, respectively. Functional categorization analysis of the human MII non-homologous genes revealed that cAMP-mediated signaling, sister chromatid cohesin, and cell recognition were the major enriched biological processes. Interestingly, we couldn’t detect any GO categories in mouse non-homologous genes. Conclusions This study demonstrates that human and mouse oocytes exhibit significant differences in gene expression profiles during oocyte maturation, which probably deciphers the differential molecular responses to oocyte maturation and fertilization. The significant differences between human and mouse oocytes limit the generalizations from mouse to human oocyte maturation. Knowledge about the limitations of animal models is crucial when exploring a complex process such as human oocyte maturation and fertilization.http://link.springer.com/article/10.1186/s12864-020-06885-4Oocyte maturationTranscriptomeFertilizationTranscripts degradation |
| spellingShingle | Zheng-Hui Zhao Tie-Gang Meng Ang Li Heide Schatten Zhen-Bo Wang Qing-Yuan Sun RNA-Seq transcriptome reveals different molecular responses during human and mouse oocyte maturation and fertilization BMC Genomics Oocyte maturation Transcriptome Fertilization Transcripts degradation |
| title | RNA-Seq transcriptome reveals different molecular responses during human and mouse oocyte maturation and fertilization |
| title_full | RNA-Seq transcriptome reveals different molecular responses during human and mouse oocyte maturation and fertilization |
| title_fullStr | RNA-Seq transcriptome reveals different molecular responses during human and mouse oocyte maturation and fertilization |
| title_full_unstemmed | RNA-Seq transcriptome reveals different molecular responses during human and mouse oocyte maturation and fertilization |
| title_short | RNA-Seq transcriptome reveals different molecular responses during human and mouse oocyte maturation and fertilization |
| title_sort | rna seq transcriptome reveals different molecular responses during human and mouse oocyte maturation and fertilization |
| topic | Oocyte maturation Transcriptome Fertilization Transcripts degradation |
| url | http://link.springer.com/article/10.1186/s12864-020-06885-4 |
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