Performance of the Disc Diffusion Method, MTS Gradient Tests and Two Commercially Available Microdilution Tests for the Determination of Cefiderocol Susceptibility in <i>Acinetobacter</i> spp.
Cefiderocol is a siderophore-conjugated cephalosporin with potent activity against multidrug-resistant Gram-negative pathogens including <i>Acinetobacter baumannii</i>. The aim of this study was to evaluate cefiderocol testing methods on a relevant collection of 97 <i>Acinetobacter...
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MDPI AG
2023-07-01
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| Online Access: | https://www.mdpi.com/2076-2607/11/8/1971 |
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| author | Katy Jeannot Susie Gaillot Pauline Triponney Sylvain Portets Valentin Pourchet Damien Fournier Anaïs Potron |
| author_facet | Katy Jeannot Susie Gaillot Pauline Triponney Sylvain Portets Valentin Pourchet Damien Fournier Anaïs Potron |
| author_sort | Katy Jeannot |
| collection | DOAJ |
| description | Cefiderocol is a siderophore-conjugated cephalosporin with potent activity against multidrug-resistant Gram-negative pathogens including <i>Acinetobacter baumannii</i>. The aim of this study was to evaluate cefiderocol testing methods on a relevant collection of 97 <i>Acinetobacter</i> spp. isolates. Commercialized broth microdilution methods (ComASP<sup>®</sup>, Liofilchem and UMIC<sup>®</sup>, Bruker), MIC test strips (Liofilchem) and disc diffusion using discs of three different brands (Mast Diagnostic, Liofilchem and Oxoid—Thermo Fisher Scientific) were compared with the broth microdilution reference method. None of the methods tested fulfilled acceptable criteria (essential agreement [EA] ≥ 90%; bias = ±30%) but both BMD methods achieved acceptable categorical agreement rates (CA = 95.9% [93/97, 95% CI 89.9–98.4] and CA = 93.8% [91/97, 95% CI 87.2–97.1] for ComASP<sup>®</sup> and UMIC<sup>®</sup>, respectively) and bias < 30% (−7.2% and −25.2% for ComASP<sup>®</sup> and UMIC<sup>®</sup>, respectively). The use of MIC gradient testing is strongly discouraged due to misclassification of 55% (<i>n </i>= 23/42) of resistant strains. Finally, the disc diffusion method could be used to rapidly screen for susceptible strains by setting a critical diameter of 22 mm. |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T23:43:55Z |
| publishDate | 2023-07-01 |
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| series | Microorganisms |
| spelling | doaj.art-b6904a68f81a48bcb8795813c9ea8db72023-11-19T02:16:58ZengMDPI AGMicroorganisms2076-26072023-07-01118197110.3390/microorganisms11081971Performance of the Disc Diffusion Method, MTS Gradient Tests and Two Commercially Available Microdilution Tests for the Determination of Cefiderocol Susceptibility in <i>Acinetobacter</i> spp.Katy Jeannot0Susie Gaillot1Pauline Triponney2Sylvain Portets3Valentin Pourchet4Damien Fournier5Anaïs Potron6Laboratoire de Bactériologie, CHU de Besançon, F-25000 Besançon, FranceCNRS, UMR 6249, Chrono-Environnement, Université de Franche-Comté, F-25000 Besançon, FranceCentre National de Référence de la Résistance aux Antibiotiques, Laboratoire Associé, Centre Hospitalier Universitaire de Besançon, F-25000 Besançon, FranceLaboratoire de Bactériologie, CHU de Besançon, F-25000 Besançon, FranceLaboratoire de Bactériologie, CHU de Besançon, F-25000 Besançon, FranceLaboratoire de Bactériologie, CHU de Besançon, F-25000 Besançon, FranceLaboratoire de Bactériologie, CHU de Besançon, F-25000 Besançon, FranceCefiderocol is a siderophore-conjugated cephalosporin with potent activity against multidrug-resistant Gram-negative pathogens including <i>Acinetobacter baumannii</i>. The aim of this study was to evaluate cefiderocol testing methods on a relevant collection of 97 <i>Acinetobacter</i> spp. isolates. Commercialized broth microdilution methods (ComASP<sup>®</sup>, Liofilchem and UMIC<sup>®</sup>, Bruker), MIC test strips (Liofilchem) and disc diffusion using discs of three different brands (Mast Diagnostic, Liofilchem and Oxoid—Thermo Fisher Scientific) were compared with the broth microdilution reference method. None of the methods tested fulfilled acceptable criteria (essential agreement [EA] ≥ 90%; bias = ±30%) but both BMD methods achieved acceptable categorical agreement rates (CA = 95.9% [93/97, 95% CI 89.9–98.4] and CA = 93.8% [91/97, 95% CI 87.2–97.1] for ComASP<sup>®</sup> and UMIC<sup>®</sup>, respectively) and bias < 30% (−7.2% and −25.2% for ComASP<sup>®</sup> and UMIC<sup>®</sup>, respectively). The use of MIC gradient testing is strongly discouraged due to misclassification of 55% (<i>n </i>= 23/42) of resistant strains. Finally, the disc diffusion method could be used to rapidly screen for susceptible strains by setting a critical diameter of 22 mm.https://www.mdpi.com/2076-2607/11/8/1971cefiderocol<i>Acinetobacter</i> spp.susceptibility testing |
| spellingShingle | Katy Jeannot Susie Gaillot Pauline Triponney Sylvain Portets Valentin Pourchet Damien Fournier Anaïs Potron Performance of the Disc Diffusion Method, MTS Gradient Tests and Two Commercially Available Microdilution Tests for the Determination of Cefiderocol Susceptibility in <i>Acinetobacter</i> spp. Microorganisms cefiderocol <i>Acinetobacter</i> spp. susceptibility testing |
| title | Performance of the Disc Diffusion Method, MTS Gradient Tests and Two Commercially Available Microdilution Tests for the Determination of Cefiderocol Susceptibility in <i>Acinetobacter</i> spp. |
| title_full | Performance of the Disc Diffusion Method, MTS Gradient Tests and Two Commercially Available Microdilution Tests for the Determination of Cefiderocol Susceptibility in <i>Acinetobacter</i> spp. |
| title_fullStr | Performance of the Disc Diffusion Method, MTS Gradient Tests and Two Commercially Available Microdilution Tests for the Determination of Cefiderocol Susceptibility in <i>Acinetobacter</i> spp. |
| title_full_unstemmed | Performance of the Disc Diffusion Method, MTS Gradient Tests and Two Commercially Available Microdilution Tests for the Determination of Cefiderocol Susceptibility in <i>Acinetobacter</i> spp. |
| title_short | Performance of the Disc Diffusion Method, MTS Gradient Tests and Two Commercially Available Microdilution Tests for the Determination of Cefiderocol Susceptibility in <i>Acinetobacter</i> spp. |
| title_sort | performance of the disc diffusion method mts gradient tests and two commercially available microdilution tests for the determination of cefiderocol susceptibility in i acinetobacter i spp |
| topic | cefiderocol <i>Acinetobacter</i> spp. susceptibility testing |
| url | https://www.mdpi.com/2076-2607/11/8/1971 |
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