Production and Characterization of Poly(3-hydroxybutyrate) from <i>Halomonas boliviensis</i> LC1 Cultivated in Hydrolysates of Quinoa Stalks
The global production of fossil-based plastics has reached critical levels, and their substitution with bio-based polymers is an urgent requirement. Poly(3-hydroxybutyrate) (PHB) is a biopolymer that can be produced via microbial cultivation, but efficient microorganisms and low-cost substrates are...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/6/556 |
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| author | Diego A. Miranda Katherine Marín Ola Sundman Mattias Hedenström Jorge Quillaguaman András Gorzsás Markus Broström Markus Carlborg Jenny Lundqvist Luis Romero-Soto Leif J. Jönsson Cristhian Carrasco Carlos Martín |
| author_facet | Diego A. Miranda Katherine Marín Ola Sundman Mattias Hedenström Jorge Quillaguaman András Gorzsás Markus Broström Markus Carlborg Jenny Lundqvist Luis Romero-Soto Leif J. Jönsson Cristhian Carrasco Carlos Martín |
| author_sort | Diego A. Miranda |
| collection | DOAJ |
| description | The global production of fossil-based plastics has reached critical levels, and their substitution with bio-based polymers is an urgent requirement. Poly(3-hydroxybutyrate) (PHB) is a biopolymer that can be produced via microbial cultivation, but efficient microorganisms and low-cost substrates are required. <i>Halomonas boliviensis</i> LC1, a moderately halophilic bacterium, is an effective PHB producer, and hydrolysates of the residual stalks of quinoa (<i>Chenopodium quinoa</i> Willd.) can be considered a cheap source of sugars for microbial fermentation processes in quinoa-producing countries. In this study, <i>H. boliviensis</i> LC1 was adapted to a cellulosic hydrolysate of quinoa stalks obtained via acid-catalyzed hydrothermal pretreatment and enzymatic saccharification. The adapted strain was cultivated in hydrolysates and synthetic media, each of them with two different initial concentrations of glucose. Cell growth, glucose consumption, and PHB formation during cultivation were assessed. The cultivation results showed an initial lag in microbial growth and glucose consumption in the quinoa hydrolysates compared to cultivation in synthetic medium, but after 33 h, the values were comparable for all media. Cultivation in hydrolysates with an initial glucose concentration of 15 g/L resulted in a higher glucose consumption rate (0.15 g/(L h) vs. 0.14 g/(L h)) and volumetric productivity of PHB (14.02 mg/(L h) vs. 10.89 mg/(L h)) than cultivation in hydrolysates with 20 g/L as the initial glucose concentration. During most of the cultivation time, the PHB yield on initial glucose was higher for cultivation in synthetic medium than in hydrolysates. The produced PHBs were characterized using advanced analytical techniques, such as high-performance size-exclusion chromatography (HPSEC), Fourier transform infrared (FTIR) spectroscopy, <sup>1</sup>H nuclear magnetic resonance (NMR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). HPSEC revealed that the molecular weight of PHB produced in the cellulosic hydrolysate was lower than that of PHB produced in synthetic medium. TGA showed higher thermal stability for PHB produced in synthetic medium than for that produced in the hydrolysate. The results of the other characterization techniques displayed comparable features for both PHB samples. The presented results show the feasibility of producing PHB from quinoa stalks with <i>H. boliviensis</i>. |
| first_indexed | 2024-03-11T02:30:00Z |
| format | Article |
| id | doaj.art-b691e22135844995877340f4e78b770b |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T02:30:00Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-b691e22135844995877340f4e78b770b2023-11-18T10:21:22ZengMDPI AGFermentation2311-56372023-06-019655610.3390/fermentation9060556Production and Characterization of Poly(3-hydroxybutyrate) from <i>Halomonas boliviensis</i> LC1 Cultivated in Hydrolysates of Quinoa StalksDiego A. Miranda0Katherine Marín1Ola Sundman2Mattias Hedenström3Jorge Quillaguaman4András Gorzsás5Markus Broström6Markus Carlborg7Jenny Lundqvist8Luis Romero-Soto9Leif J. Jönsson10Cristhian Carrasco11Carlos Martín12Department of Chemistry, Umeå University, SE-901 87 Umeå, SwedenDepartment of Chemistry, Umeå University, SE-901 87 Umeå, SwedenDepartment of Chemistry, Umeå University, SE-901 87 Umeå, SwedenDepartment of Chemistry, Umeå University, SE-901 87 Umeå, SwedenPlanta Piloto de Bioprocesos, Facultad de Ciencias y Tecnología, Universidad Mayor de San Simón, Cochabamba 2500, BoliviaDepartment of Chemistry, Umeå University, SE-901 87 Umeå, SwedenThermochemical Energy Conversion Laboratory, Department of Applied Physics and Electronics, Umeå University, SE-901 87 Umeå, SwedenThermochemical Energy Conversion Laboratory, Department of Applied Physics and Electronics, Umeå University, SE-901 87 Umeå, SwedenDepartment of Chemistry, Umeå University, SE-901 87 Umeå, SwedenInstituto de Investigación y Desarrollo de Procesos Químicos, Chemical Engineering, Faculty of Engineering, Universidad Mayor de San Andrés, La Paz P.O. Box 12958, BoliviaDepartment of Chemistry, Umeå University, SE-901 87 Umeå, SwedenInstituto de Investigación y Desarrollo de Procesos Químicos, Chemical Engineering, Faculty of Engineering, Universidad Mayor de San Andrés, La Paz P.O. Box 12958, BoliviaDepartment of Chemistry, Umeå University, SE-901 87 Umeå, SwedenThe global production of fossil-based plastics has reached critical levels, and their substitution with bio-based polymers is an urgent requirement. Poly(3-hydroxybutyrate) (PHB) is a biopolymer that can be produced via microbial cultivation, but efficient microorganisms and low-cost substrates are required. <i>Halomonas boliviensis</i> LC1, a moderately halophilic bacterium, is an effective PHB producer, and hydrolysates of the residual stalks of quinoa (<i>Chenopodium quinoa</i> Willd.) can be considered a cheap source of sugars for microbial fermentation processes in quinoa-producing countries. In this study, <i>H. boliviensis</i> LC1 was adapted to a cellulosic hydrolysate of quinoa stalks obtained via acid-catalyzed hydrothermal pretreatment and enzymatic saccharification. The adapted strain was cultivated in hydrolysates and synthetic media, each of them with two different initial concentrations of glucose. Cell growth, glucose consumption, and PHB formation during cultivation were assessed. The cultivation results showed an initial lag in microbial growth and glucose consumption in the quinoa hydrolysates compared to cultivation in synthetic medium, but after 33 h, the values were comparable for all media. Cultivation in hydrolysates with an initial glucose concentration of 15 g/L resulted in a higher glucose consumption rate (0.15 g/(L h) vs. 0.14 g/(L h)) and volumetric productivity of PHB (14.02 mg/(L h) vs. 10.89 mg/(L h)) than cultivation in hydrolysates with 20 g/L as the initial glucose concentration. During most of the cultivation time, the PHB yield on initial glucose was higher for cultivation in synthetic medium than in hydrolysates. The produced PHBs were characterized using advanced analytical techniques, such as high-performance size-exclusion chromatography (HPSEC), Fourier transform infrared (FTIR) spectroscopy, <sup>1</sup>H nuclear magnetic resonance (NMR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). HPSEC revealed that the molecular weight of PHB produced in the cellulosic hydrolysate was lower than that of PHB produced in synthetic medium. TGA showed higher thermal stability for PHB produced in synthetic medium than for that produced in the hydrolysate. The results of the other characterization techniques displayed comparable features for both PHB samples. The presented results show the feasibility of producing PHB from quinoa stalks with <i>H. boliviensis</i>.https://www.mdpi.com/2311-5637/9/6/556polyhydroxybutyrateagricultural residueslignocellulosic materialsquinoa<i>Halomonas boliviensis</i>halophilic bacteria |
| spellingShingle | Diego A. Miranda Katherine Marín Ola Sundman Mattias Hedenström Jorge Quillaguaman András Gorzsás Markus Broström Markus Carlborg Jenny Lundqvist Luis Romero-Soto Leif J. Jönsson Cristhian Carrasco Carlos Martín Production and Characterization of Poly(3-hydroxybutyrate) from <i>Halomonas boliviensis</i> LC1 Cultivated in Hydrolysates of Quinoa Stalks Fermentation polyhydroxybutyrate agricultural residues lignocellulosic materials quinoa <i>Halomonas boliviensis</i> halophilic bacteria |
| title | Production and Characterization of Poly(3-hydroxybutyrate) from <i>Halomonas boliviensis</i> LC1 Cultivated in Hydrolysates of Quinoa Stalks |
| title_full | Production and Characterization of Poly(3-hydroxybutyrate) from <i>Halomonas boliviensis</i> LC1 Cultivated in Hydrolysates of Quinoa Stalks |
| title_fullStr | Production and Characterization of Poly(3-hydroxybutyrate) from <i>Halomonas boliviensis</i> LC1 Cultivated in Hydrolysates of Quinoa Stalks |
| title_full_unstemmed | Production and Characterization of Poly(3-hydroxybutyrate) from <i>Halomonas boliviensis</i> LC1 Cultivated in Hydrolysates of Quinoa Stalks |
| title_short | Production and Characterization of Poly(3-hydroxybutyrate) from <i>Halomonas boliviensis</i> LC1 Cultivated in Hydrolysates of Quinoa Stalks |
| title_sort | production and characterization of poly 3 hydroxybutyrate from i halomonas boliviensis i lc1 cultivated in hydrolysates of quinoa stalks |
| topic | polyhydroxybutyrate agricultural residues lignocellulosic materials quinoa <i>Halomonas boliviensis</i> halophilic bacteria |
| url | https://www.mdpi.com/2311-5637/9/6/556 |
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