The Effect of Severe Periodic Training and Crocin on Oxidative Stress in Male Rats Subjected to Doxorubicin Induction
Abstract: Background & aim: Cardiovascular disease is one of the principal causes of mortality in the world. Oxidative stress plays a major role in the development of cardiovascular diseases. Physical activity improves the antioxidant status of the heart tissue. The aim of the present study was...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | fas |
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Yasuj University Of Medical Sciences
2019-01-01
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| Series: | Armaghane Danesh Bimonthly Journal |
| Subjects: | |
| Online Access: | http://armaghanj.yums.ac.ir/article-1-2182-en.html |
| _version_ | 1811205250925723648 |
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| author | R Khanmohammadi MA Azarbaijani M Piri L Khorsandi |
| author_facet | R Khanmohammadi MA Azarbaijani M Piri L Khorsandi |
| author_sort | R Khanmohammadi |
| collection | DOAJ |
| description | Abstract:
Background & aim: Cardiovascular disease is one of the principal causes of mortality in the world. Oxidative stress plays a major role in the development of cardiovascular diseases. Physical activity improves the antioxidant status of the heart tissue. The aim of the present study was to investigate the levels of oxidative and antioxidant indices of the heart tissue of exposed male rats exposed to doxorubicin after eight weeks of severe routine training and consumption of crocin.
Methods: In the present experimental study, 50 male Wistar rats with a mean age of 8 weeks and an average weight of 200-220 g were randomly assigned to 5 healthy control groups (saline), doxorubicin (control), doxorubicin crocin, doxorubicin training, and doxorubicin crocin training. The training groups completed the course with two severe periods in the first week and completed 8 severe periods in the last weeks. After 8 weeks, malondialdehyde levels (MDA) and superoxide dismutase (SOD) and catalase (CAT) enzymes activity were measured in cardiac tissue. Data were analyzed by independent t-test, two way ANOVA.
Results: Data analysis indicated a significant increase in malondialdehyde level and a significant decrease in the activity of superoxide dismutase and heart catalase in Doxorubicin group compared to healthy control group (p=0.001). Moreover, the results revealed a significant decrease (P=0.001), crocin (p=0.001), and the combination of exercise and crocin (p=0.025), and a significant increase in the activity of superoxide dismutase and catalase. In the result it was found that exercise (p=0.001), crocin (p=0.001), and exercise combinations (p=0.005) showed that in the comparison between the groups, the effect of exercise and crocin alone success. There was no significant difference, but there was a significant difference between their combined effect and the effect of each of these two interventions (exercise and crocin) alone, and these two effects amplified each other (p=0.001).
Conclusion: It seems that regular high intensity interval training, supplementation of crocin, or a combination of these two, could be achieved by decreasing levels of oxidative index, malondialdehyde and increased activity of antioxidant enzymes of superoxide dismutase and catalase of heart tissue, protective effects against oxidative damage of doxorubicin have. It appeared that combining these two interventions may have a better preventive and therapeutic strategy than their one-way use. |
| first_indexed | 2024-04-12T03:27:28Z |
| format | Article |
| id | doaj.art-b692be5315a5454c9a0ddd24345696a2 |
| institution | Directory Open Access Journal |
| issn | 1728-6514 1728-6514 |
| language | fas |
| last_indexed | 2024-04-12T03:27:28Z |
| publishDate | 2019-01-01 |
| publisher | Yasuj University Of Medical Sciences |
| record_format | Article |
| series | Armaghane Danesh Bimonthly Journal |
| spelling | doaj.art-b692be5315a5454c9a0ddd24345696a22022-12-22T03:49:37ZfasYasuj University Of Medical SciencesArmaghane Danesh Bimonthly Journal1728-65141728-65142019-01-01236694708The Effect of Severe Periodic Training and Crocin on Oxidative Stress in Male Rats Subjected to Doxorubicin InductionR Khanmohammadi0MA Azarbaijani1M Piri2L Khorsandi3 Department of Physiolysis, Central Tehran University, Islamic Azad University, Tehran, Iran Department of Physiolysis, Central Tehran University, Islamic Azad University, Tehran, Iran Department of Physiolysis, Central Tehran University, Islamic Azad University, Tehran, Iran Department of Anatomical Sciences, Ahwaz Jundishapur University of Medical Sciences, Ahvaz, Iran Abstract: Background & aim: Cardiovascular disease is one of the principal causes of mortality in the world. Oxidative stress plays a major role in the development of cardiovascular diseases. Physical activity improves the antioxidant status of the heart tissue. The aim of the present study was to investigate the levels of oxidative and antioxidant indices of the heart tissue of exposed male rats exposed to doxorubicin after eight weeks of severe routine training and consumption of crocin. Methods: In the present experimental study, 50 male Wistar rats with a mean age of 8 weeks and an average weight of 200-220 g were randomly assigned to 5 healthy control groups (saline), doxorubicin (control), doxorubicin crocin, doxorubicin training, and doxorubicin crocin training. The training groups completed the course with two severe periods in the first week and completed 8 severe periods in the last weeks. After 8 weeks, malondialdehyde levels (MDA) and superoxide dismutase (SOD) and catalase (CAT) enzymes activity were measured in cardiac tissue. Data were analyzed by independent t-test, two way ANOVA. Results: Data analysis indicated a significant increase in malondialdehyde level and a significant decrease in the activity of superoxide dismutase and heart catalase in Doxorubicin group compared to healthy control group (p=0.001). Moreover, the results revealed a significant decrease (P=0.001), crocin (p=0.001), and the combination of exercise and crocin (p=0.025), and a significant increase in the activity of superoxide dismutase and catalase. In the result it was found that exercise (p=0.001), crocin (p=0.001), and exercise combinations (p=0.005) showed that in the comparison between the groups, the effect of exercise and crocin alone success. There was no significant difference, but there was a significant difference between their combined effect and the effect of each of these two interventions (exercise and crocin) alone, and these two effects amplified each other (p=0.001). Conclusion: It seems that regular high intensity interval training, supplementation of crocin, or a combination of these two, could be achieved by decreasing levels of oxidative index, malondialdehyde and increased activity of antioxidant enzymes of superoxide dismutase and catalase of heart tissue, protective effects against oxidative damage of doxorubicin have. It appeared that combining these two interventions may have a better preventive and therapeutic strategy than their one-way use.http://armaghanj.yums.ac.ir/article-1-2182-en.htmlSevere periodic exerciseCrocinDoxorubicinOxidative stress |
| spellingShingle | R Khanmohammadi MA Azarbaijani M Piri L Khorsandi The Effect of Severe Periodic Training and Crocin on Oxidative Stress in Male Rats Subjected to Doxorubicin Induction Armaghane Danesh Bimonthly Journal Severe periodic exercise Crocin Doxorubicin Oxidative stress |
| title | The Effect of Severe Periodic Training and Crocin on Oxidative Stress in Male Rats Subjected to Doxorubicin Induction |
| title_full | The Effect of Severe Periodic Training and Crocin on Oxidative Stress in Male Rats Subjected to Doxorubicin Induction |
| title_fullStr | The Effect of Severe Periodic Training and Crocin on Oxidative Stress in Male Rats Subjected to Doxorubicin Induction |
| title_full_unstemmed | The Effect of Severe Periodic Training and Crocin on Oxidative Stress in Male Rats Subjected to Doxorubicin Induction |
| title_short | The Effect of Severe Periodic Training and Crocin on Oxidative Stress in Male Rats Subjected to Doxorubicin Induction |
| title_sort | effect of severe periodic training and crocin on oxidative stress in male rats subjected to doxorubicin induction |
| topic | Severe periodic exercise Crocin Doxorubicin Oxidative stress |
| url | http://armaghanj.yums.ac.ir/article-1-2182-en.html |
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