Novel design for a microfluidic-based platform for yeast replicative lifespan (RLS) analysis
Microfluidic devices hold enormous potential for the development of cost-effective and faster alternatives to existing traditional methods across life science applications. Here we demonstrate the feasibility of fabricating a microfluidic device by means of photolithography comprising a single cell...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Micro and Nano Engineering |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590007223000291 |
| _version_ | 1797798690550710272 |
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| author | Georgia D. Kaprou Abhay Andar Pranjul Shah Carole L. Linster Nicole Paczia |
| author_facet | Georgia D. Kaprou Abhay Andar Pranjul Shah Carole L. Linster Nicole Paczia |
| author_sort | Georgia D. Kaprou |
| collection | DOAJ |
| description | Microfluidic devices hold enormous potential for the development of cost-effective and faster alternatives to existing traditional methods across life science applications. Here we demonstrate the feasibility of fabricating a microfluidic device by means of photolithography comprising a single cell trap, a delay structure and a chamber defined by micropillars. This device is aimed to be used for biological applications such as replicative lifespan determination (RLS) of yeast cells, where single cell trapping, and cell counting are essential. The novelty of the present work lies on the integration of the above-mentioned microfluidic structures in a single device by means of the established method of photolithography by fine-tuning critical parameters needed to achieve the desired high aspect ratio (1:5) employing commercially available resins. The fine-tuning of the fabrication parameters in combination with appropriately selected resins allows for patterning reproducibly micron-sized features. The design of the proposed device ultimately aims at replacing the very cumbersome assays still commonly used today for RLS determination in budding yeast by a methodology that is drastically simpler and more time efficient. |
| first_indexed | 2024-03-13T04:08:39Z |
| format | Article |
| id | doaj.art-b693211722844622935b085ff471d6ff |
| institution | Directory Open Access Journal |
| issn | 2590-0072 |
| language | English |
| last_indexed | 2024-03-13T04:08:39Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Micro and Nano Engineering |
| spelling | doaj.art-b693211722844622935b085ff471d6ff2023-06-21T06:58:56ZengElsevierMicro and Nano Engineering2590-00722023-06-0119100199Novel design for a microfluidic-based platform for yeast replicative lifespan (RLS) analysisGeorgia D. Kaprou0Abhay Andar1Pranjul Shah2Carole L. Linster3Nicole Paczia4Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Belvaux 4367, LuxembourgCenter for Advanced Sensor Technology, University of Maryland Baltimore County, Baltimore, MD 21250, United States; Potomac Photonics Inc., BWTech Parkway South Campus, 1450 South Rolling Road, Baltimore, MD 20008, United States of AmericaLuxembourg Centre for Systems Biomedicine, University of Luxembourg, Belvaux 4367, LuxembourgLuxembourg Centre for Systems Biomedicine, University of Luxembourg, Belvaux 4367, LuxembourgLuxembourg Centre for Systems Biomedicine, University of Luxembourg, Belvaux 4367, Luxembourg; Core Facility for Metabolomics and Small Molecule Mass Spectrometry, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany; Corresponding author at: Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Belvaux 4367, Luxembourg, Core Facility for Metabolomics and Small Molecule Mass Spectrometry, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.Microfluidic devices hold enormous potential for the development of cost-effective and faster alternatives to existing traditional methods across life science applications. Here we demonstrate the feasibility of fabricating a microfluidic device by means of photolithography comprising a single cell trap, a delay structure and a chamber defined by micropillars. This device is aimed to be used for biological applications such as replicative lifespan determination (RLS) of yeast cells, where single cell trapping, and cell counting are essential. The novelty of the present work lies on the integration of the above-mentioned microfluidic structures in a single device by means of the established method of photolithography by fine-tuning critical parameters needed to achieve the desired high aspect ratio (1:5) employing commercially available resins. The fine-tuning of the fabrication parameters in combination with appropriately selected resins allows for patterning reproducibly micron-sized features. The design of the proposed device ultimately aims at replacing the very cumbersome assays still commonly used today for RLS determination in budding yeast by a methodology that is drastically simpler and more time efficient.http://www.sciencedirect.com/science/article/pii/S2590007223000291MicrofluidicsYeastPhotolithographySingle cell trappingReplicative lifespan |
| spellingShingle | Georgia D. Kaprou Abhay Andar Pranjul Shah Carole L. Linster Nicole Paczia Novel design for a microfluidic-based platform for yeast replicative lifespan (RLS) analysis Micro and Nano Engineering Microfluidics Yeast Photolithography Single cell trapping Replicative lifespan |
| title | Novel design for a microfluidic-based platform for yeast replicative lifespan (RLS) analysis |
| title_full | Novel design for a microfluidic-based platform for yeast replicative lifespan (RLS) analysis |
| title_fullStr | Novel design for a microfluidic-based platform for yeast replicative lifespan (RLS) analysis |
| title_full_unstemmed | Novel design for a microfluidic-based platform for yeast replicative lifespan (RLS) analysis |
| title_short | Novel design for a microfluidic-based platform for yeast replicative lifespan (RLS) analysis |
| title_sort | novel design for a microfluidic based platform for yeast replicative lifespan rls analysis |
| topic | Microfluidics Yeast Photolithography Single cell trapping Replicative lifespan |
| url | http://www.sciencedirect.com/science/article/pii/S2590007223000291 |
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