Effect of systemic administration of Bifidobacterium animalis subsp. lactis HN019 on apical periodontitis
Abstract This study aimed to evaluate the effect of Bifidobacterium animalis subsp. lactis (B. lactis) HN019 in drinking water on the development of apical periodontitis (AP) in rats. In total 60 animals were divided into a control group (sound teeth); Group I - regular water without AP; Group II -...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Pesquisa Odontológica
2023-11-01
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| Series: | Brazilian Oral Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242023000100297&tlng=en |
| _version_ | 1797628802300379136 |
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| author | Lisa Danielly Curcino ARAUJO Raquel Assed Bezerra SEGATO Thaís de Paula Colen REIS Sérgio Luis de Souza SALVADOR Flávia Aparecida Chaves FURLANETO Michel Reis MESSORA Paulo NELSON-FILHO Lana Kei Yamamoto de ALMEIDA Marília Pacífico LUCISANO Clara Marina Pereira Cavalcanti SILVA Léa Assed Bezerra da SILVA |
| author_facet | Lisa Danielly Curcino ARAUJO Raquel Assed Bezerra SEGATO Thaís de Paula Colen REIS Sérgio Luis de Souza SALVADOR Flávia Aparecida Chaves FURLANETO Michel Reis MESSORA Paulo NELSON-FILHO Lana Kei Yamamoto de ALMEIDA Marília Pacífico LUCISANO Clara Marina Pereira Cavalcanti SILVA Léa Assed Bezerra da SILVA |
| author_sort | Lisa Danielly Curcino ARAUJO |
| collection | DOAJ |
| description | Abstract This study aimed to evaluate the effect of Bifidobacterium animalis subsp. lactis (B. lactis) HN019 in drinking water on the development of apical periodontitis (AP) in rats. In total 60 animals were divided into a control group (sound teeth); Group I - regular water without AP; Group II - probiotic water without AP; Group III - regular water with AP; Group IV - probiotic water with AP. AP was induced after 3 days in the control groups and after 7, 21, and 42 days in groups III and IV. The animals were euthanized, and the mandibles were subjected to histotechnical processing. Samples were stained with hematoxylin & eosin (H&E) to identify root canal features, apical and periapical regions. Additionally, histoenzymology was performed to detect osteoclasts, immunohistochemistry was used to identify osteoclastogenesis markers, and the Brown & Brenn technique was applied for microbiological analysis. The data were analyzed using GraphPad Prism 8.0.1 with a significance level of 5%. Although no statistical differences were observed, the groups administered with probiotics showed better conditions in terms of histological aspects seen microscopically. Furthermore, there were no differences in the number of osteoclasts (p > 0.05). The RANKL marker was not found in the probiotic group at 42 days, unlike in group III. |
| first_indexed | 2024-03-11T10:45:15Z |
| format | Article |
| id | doaj.art-b694b130db7343e39f32d50ccd51dfb4 |
| institution | Directory Open Access Journal |
| issn | 1807-3107 |
| language | English |
| last_indexed | 2024-03-11T10:45:15Z |
| publishDate | 2023-11-01 |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-b694b130db7343e39f32d50ccd51dfb42023-11-14T07:44:13ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-31072023-11-013710.1590/1807-3107bor-2023.vol37.0115Effect of systemic administration of Bifidobacterium animalis subsp. lactis HN019 on apical periodontitisLisa Danielly Curcino ARAUJOhttps://orcid.org/0000-0003-0014-6715Raquel Assed Bezerra SEGATOhttps://orcid.org/0000-0002-0230-1347Thaís de Paula Colen REIShttps://orcid.org/0000-0002-9005-359XSérgio Luis de Souza SALVADORhttps://orcid.org/0000-0002-4867-7294Flávia Aparecida Chaves FURLANETOhttps://orcid.org/0000-0002-8072-3983Michel Reis MESSORAhttps://orcid.org/0000-0001-8485-9645Paulo NELSON-FILHOhttps://orcid.org/0000-0001-8802-6480Lana Kei Yamamoto de ALMEIDAhttps://orcid.org/0000-0002-6308-0613Marília Pacífico LUCISANOhttps://orcid.org/0000-0002-6866-0561Clara Marina Pereira Cavalcanti SILVAhttps://orcid.org/0000-0001-5099-1074Léa Assed Bezerra da SILVAhttps://orcid.org/0000-0001-7118-6859Abstract This study aimed to evaluate the effect of Bifidobacterium animalis subsp. lactis (B. lactis) HN019 in drinking water on the development of apical periodontitis (AP) in rats. In total 60 animals were divided into a control group (sound teeth); Group I - regular water without AP; Group II - probiotic water without AP; Group III - regular water with AP; Group IV - probiotic water with AP. AP was induced after 3 days in the control groups and after 7, 21, and 42 days in groups III and IV. The animals were euthanized, and the mandibles were subjected to histotechnical processing. Samples were stained with hematoxylin & eosin (H&E) to identify root canal features, apical and periapical regions. Additionally, histoenzymology was performed to detect osteoclasts, immunohistochemistry was used to identify osteoclastogenesis markers, and the Brown & Brenn technique was applied for microbiological analysis. The data were analyzed using GraphPad Prism 8.0.1 with a significance level of 5%. Although no statistical differences were observed, the groups administered with probiotics showed better conditions in terms of histological aspects seen microscopically. Furthermore, there were no differences in the number of osteoclasts (p > 0.05). The RANKL marker was not found in the probiotic group at 42 days, unlike in group III.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242023000100297&tlng=enProbioticsPeriapical PeriodontitisEndodonticsBifidobacterium |
| spellingShingle | Lisa Danielly Curcino ARAUJO Raquel Assed Bezerra SEGATO Thaís de Paula Colen REIS Sérgio Luis de Souza SALVADOR Flávia Aparecida Chaves FURLANETO Michel Reis MESSORA Paulo NELSON-FILHO Lana Kei Yamamoto de ALMEIDA Marília Pacífico LUCISANO Clara Marina Pereira Cavalcanti SILVA Léa Assed Bezerra da SILVA Effect of systemic administration of Bifidobacterium animalis subsp. lactis HN019 on apical periodontitis Brazilian Oral Research Probiotics Periapical Periodontitis Endodontics Bifidobacterium |
| title | Effect of systemic administration of Bifidobacterium animalis subsp. lactis HN019 on apical periodontitis |
| title_full | Effect of systemic administration of Bifidobacterium animalis subsp. lactis HN019 on apical periodontitis |
| title_fullStr | Effect of systemic administration of Bifidobacterium animalis subsp. lactis HN019 on apical periodontitis |
| title_full_unstemmed | Effect of systemic administration of Bifidobacterium animalis subsp. lactis HN019 on apical periodontitis |
| title_short | Effect of systemic administration of Bifidobacterium animalis subsp. lactis HN019 on apical periodontitis |
| title_sort | effect of systemic administration of bifidobacterium animalis subsp lactis hn019 on apical periodontitis |
| topic | Probiotics Periapical Periodontitis Endodontics Bifidobacterium |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242023000100297&tlng=en |
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