Protective efficacy of anti-neuraminidase monoclonal antibodies against H7N9 influenza virus infection
ABSTRACTThe H7N9 influenza virus has been circulating in China for more than six years. The neuraminidase (NA) has gained great concern for the development of antiviral drugs, therapeutic antibodies, and new vaccines. In this study, we screened seven mouse monoclonal antibodies (mAbs) and compared t...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2019.1708214 |
| _version_ | 1797265823638749184 |
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| author | Fei-Fei Xiong Xue-Ying Liu Fei-Xia Gao Jian Luo Peng Duan Wen-Song Tan Ze Chen |
| author_facet | Fei-Fei Xiong Xue-Ying Liu Fei-Xia Gao Jian Luo Peng Duan Wen-Song Tan Ze Chen |
| author_sort | Fei-Fei Xiong |
| collection | DOAJ |
| description | ABSTRACTThe H7N9 influenza virus has been circulating in China for more than six years. The neuraminidase (NA) has gained great concern for the development of antiviral drugs, therapeutic antibodies, and new vaccines. In this study, we screened seven mouse monoclonal antibodies (mAbs) and compared their protective effects against H7N9 influenza virus. The epitope mapping from escape mutants showed that all the seven mAbs could bind to the head region of the N9 NA close to the enzyme activity sites, and four key sites of N9 NA were reported for the first time. The mAbs D3 and 7H2 could simultaneously inhibit the cleavage of the sialic acid of fetuin protein with large molecular weight and NA-XTD with small molecule weight in the NA inhibition experiment, prevent the formation of virus plaque at a low concentration, and effectively protect the mice from the challenge of the lethal dose of H7N9 virus. |
| first_indexed | 2024-03-07T17:24:19Z |
| format | Article |
| id | doaj.art-b6967c4e02894ecb8493d536d2b55c4c |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-04-25T00:50:55Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-b6967c4e02894ecb8493d536d2b55c4c2024-03-11T16:04:25ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-0191788710.1080/22221751.2019.1708214Protective efficacy of anti-neuraminidase monoclonal antibodies against H7N9 influenza virus infectionFei-Fei Xiong0Xue-Ying Liu1Fei-Xia Gao2Jian Luo3Peng Duan4Wen-Song Tan5Ze Chen6Shanghai Institute of Biological Products, Shanghai, People’s Republic of ChinaShanghai Institute of Biological Products, Shanghai, People’s Republic of ChinaShanghai Institute of Biological Products, Shanghai, People’s Republic of ChinaShanghai Institute of Biological Products, Shanghai, People’s Republic of ChinaShanghai Institute of Biological Products, Shanghai, People’s Republic of ChinaEast China University of Science and Technology, Shanghai, People’s Republic of ChinaShanghai Institute of Biological Products, Shanghai, People’s Republic of ChinaABSTRACTThe H7N9 influenza virus has been circulating in China for more than six years. The neuraminidase (NA) has gained great concern for the development of antiviral drugs, therapeutic antibodies, and new vaccines. In this study, we screened seven mouse monoclonal antibodies (mAbs) and compared their protective effects against H7N9 influenza virus. The epitope mapping from escape mutants showed that all the seven mAbs could bind to the head region of the N9 NA close to the enzyme activity sites, and four key sites of N9 NA were reported for the first time. The mAbs D3 and 7H2 could simultaneously inhibit the cleavage of the sialic acid of fetuin protein with large molecular weight and NA-XTD with small molecule weight in the NA inhibition experiment, prevent the formation of virus plaque at a low concentration, and effectively protect the mice from the challenge of the lethal dose of H7N9 virus.https://www.tandfonline.com/doi/10.1080/22221751.2019.1708214H7N9 influenza virusneuraminidasemonoclonal antibodiesprotectionNA epitope |
| spellingShingle | Fei-Fei Xiong Xue-Ying Liu Fei-Xia Gao Jian Luo Peng Duan Wen-Song Tan Ze Chen Protective efficacy of anti-neuraminidase monoclonal antibodies against H7N9 influenza virus infection Emerging Microbes and Infections H7N9 influenza virus neuraminidase monoclonal antibodies protection NA epitope |
| title | Protective efficacy of anti-neuraminidase monoclonal antibodies against H7N9 influenza virus infection |
| title_full | Protective efficacy of anti-neuraminidase monoclonal antibodies against H7N9 influenza virus infection |
| title_fullStr | Protective efficacy of anti-neuraminidase monoclonal antibodies against H7N9 influenza virus infection |
| title_full_unstemmed | Protective efficacy of anti-neuraminidase monoclonal antibodies against H7N9 influenza virus infection |
| title_short | Protective efficacy of anti-neuraminidase monoclonal antibodies against H7N9 influenza virus infection |
| title_sort | protective efficacy of anti neuraminidase monoclonal antibodies against h7n9 influenza virus infection |
| topic | H7N9 influenza virus neuraminidase monoclonal antibodies protection NA epitope |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2019.1708214 |
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