Comparison of two methods fordetection of E. coli O157H7 in unpasteurized milk
Background and Objectives: The most common serotype of enterohaemorrhagic Esherichia coli group or Shiga-toxin-producing E. coli is O157:H7. Domestic and wild ruminants are regarded as the main natural reservoirs. O157:H7 serotype is the major cause of gastrointestinal infections in developed countr...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2016-12-01
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| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/825 |
| _version_ | 1819241408795508736 |
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| author | Maryam Farahmandfar Naghmeh Moori-Bakhtiari Saad Gooraninezhad Mehdi Zarei |
| author_facet | Maryam Farahmandfar Naghmeh Moori-Bakhtiari Saad Gooraninezhad Mehdi Zarei |
| author_sort | Maryam Farahmandfar |
| collection | DOAJ |
| description | Background and Objectives: The most common serotype of enterohaemorrhagic Esherichia coli group or Shiga-toxin-producing E. coli is O157:H7. Domestic and wild ruminants are regarded as the main natural reservoirs. O157:H7 serotype is the major cause of gastrointestinal infections in developed countries. In this study was conducted to survey on the toxigenic E. coli O157: H7 strains in milk of industrial dairy farms.
Materials and Methods: A total number of 150 milk samples were collected from dairy industry in Khuzestan, over a period of 6 months and were evaluated by cultivation in selective media (CT-SMAC) and multiplex PCR.
Results: Two isolates were identified as E. coli using biochemical tests, none of them were toxigenic E. coli O157:H7 as determined by multiplex PCR. Using direct PCR on milk samples, 45 samples contained at least one gene of the studied genes in this investigation (rfb, flic, stx1, stx2). With direct PCR, 2 milk samples were positive for toxigenic O157:H7.
Conclusion: E. coli O157:H7 is present in this region and so the necessity for strict compliance of health standards is recommended. This is the first study on O157: H7 E. coli milk contamination in Khuzestan province. Based on these results, direct PCR is more accurate than indirect PCR. |
| first_indexed | 2024-12-23T14:23:26Z |
| format | Article |
| id | doaj.art-b697574bf17f422a8707d2993884bf8d |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-12-23T14:23:26Z |
| publishDate | 2016-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-b697574bf17f422a8707d2993884bf8d2022-12-21T17:43:44ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472016-12-0185Comparison of two methods fordetection of E. coli O157H7 in unpasteurized milkMaryam Farahmandfar0Naghmeh Moori-Bakhtiari1Saad Gooraninezhad2Mehdi Zarei3Departments of Microbiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz ,IranDepartment of Microbiology, Faculty of Veterinary Medicine, shahid chamran University of Ahvaz, IranDepartment of clinical sciences, faculty of veterinary medicine, shahid chamran university of Ahvaz, IranDepartment of food hygiene, faculty of veterinary medicine, shahid chamran university of Ahvaz, IranBackground and Objectives: The most common serotype of enterohaemorrhagic Esherichia coli group or Shiga-toxin-producing E. coli is O157:H7. Domestic and wild ruminants are regarded as the main natural reservoirs. O157:H7 serotype is the major cause of gastrointestinal infections in developed countries. In this study was conducted to survey on the toxigenic E. coli O157: H7 strains in milk of industrial dairy farms. Materials and Methods: A total number of 150 milk samples were collected from dairy industry in Khuzestan, over a period of 6 months and were evaluated by cultivation in selective media (CT-SMAC) and multiplex PCR. Results: Two isolates were identified as E. coli using biochemical tests, none of them were toxigenic E. coli O157:H7 as determined by multiplex PCR. Using direct PCR on milk samples, 45 samples contained at least one gene of the studied genes in this investigation (rfb, flic, stx1, stx2). With direct PCR, 2 milk samples were positive for toxigenic O157:H7. Conclusion: E. coli O157:H7 is present in this region and so the necessity for strict compliance of health standards is recommended. This is the first study on O157: H7 E. coli milk contamination in Khuzestan province. Based on these results, direct PCR is more accurate than indirect PCR.https://ijm.tums.ac.ir/index.php/ijm/article/view/825MilkEscherichia coli O157H7PCR |
| spellingShingle | Maryam Farahmandfar Naghmeh Moori-Bakhtiari Saad Gooraninezhad Mehdi Zarei Comparison of two methods fordetection of E. coli O157H7 in unpasteurized milk Iranian Journal of Microbiology Milk Escherichia coli O157 H7 PCR |
| title | Comparison of two methods fordetection of E. coli O157H7 in unpasteurized milk |
| title_full | Comparison of two methods fordetection of E. coli O157H7 in unpasteurized milk |
| title_fullStr | Comparison of two methods fordetection of E. coli O157H7 in unpasteurized milk |
| title_full_unstemmed | Comparison of two methods fordetection of E. coli O157H7 in unpasteurized milk |
| title_short | Comparison of two methods fordetection of E. coli O157H7 in unpasteurized milk |
| title_sort | comparison of two methods fordetection of e coli o157h7 in unpasteurized milk |
| topic | Milk Escherichia coli O157 H7 PCR |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/825 |
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