Genotyping Rotavirus RNA from Archived Rotavirus-Positive Rapid Test Strips
Genotyping circulating rotaviruses before and after introduction of rotavirus vaccine is useful for evaluating vaccine-associated changes in genotype distribution. We determined frequency of rotavirus genotypes among 61 rotavirus-positive children hospitalized in Israel during the 2005–06 rotavirus...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2011-01-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/17/1/10-1132_article |
| _version_ | 1818388183689199616 |
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| author | Lester M. Shulman Ilana Silberstein Jacqueline Alfandari Ella Mendelson |
| author_facet | Lester M. Shulman Ilana Silberstein Jacqueline Alfandari Ella Mendelson |
| author_sort | Lester M. Shulman |
| collection | DOAJ |
| description | Genotyping circulating rotaviruses before and after introduction of rotavirus vaccine is useful for evaluating vaccine-associated changes in genotype distribution. We determined frequency of rotavirus genotypes among 61 rotavirus-positive children hospitalized in Israel during the 2005–06 rotavirus season. Accurate molecular epidemiologic data were recovered from affinity-concentrated rotavirus immobilized in rotavirus-positive bands from air-dried, diagnostic rotavirus rapid test strips (dipstick) stored at room temperature from 1 week to 5 years. G genotypes were identical for 21 paired dipsticks and suspensions, whereas dipsticks or suspensions detected an additional G genotype in 2 samples. RNA sequences from 7 pairs were identical. Phylogenetic analysis suggested previously unreported G2 sublineages and G9 lineages. The ease with which dipsticks can be stored at local facilities and transported to central reference laboratories can reverse increasing difficulties in obtaining geographically representative stool samples and expand surveillance to regions lacking adequate laboratory facilities. |
| first_indexed | 2024-12-14T04:21:48Z |
| format | Article |
| id | doaj.art-b6998c64cb5c40b0ae2a59213089270f |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-14T04:21:48Z |
| publishDate | 2011-01-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-b6998c64cb5c40b0ae2a59213089270f2022-12-21T23:17:21ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592011-01-01171444810.3201/eid1701.101132Genotyping Rotavirus RNA from Archived Rotavirus-Positive Rapid Test StripsLester M. ShulmanIlana SilbersteinJacqueline AlfandariElla MendelsonGenotyping circulating rotaviruses before and after introduction of rotavirus vaccine is useful for evaluating vaccine-associated changes in genotype distribution. We determined frequency of rotavirus genotypes among 61 rotavirus-positive children hospitalized in Israel during the 2005–06 rotavirus season. Accurate molecular epidemiologic data were recovered from affinity-concentrated rotavirus immobilized in rotavirus-positive bands from air-dried, diagnostic rotavirus rapid test strips (dipstick) stored at room temperature from 1 week to 5 years. G genotypes were identical for 21 paired dipsticks and suspensions, whereas dipsticks or suspensions detected an additional G genotype in 2 samples. RNA sequences from 7 pairs were identical. Phylogenetic analysis suggested previously unreported G2 sublineages and G9 lineages. The ease with which dipsticks can be stored at local facilities and transported to central reference laboratories can reverse increasing difficulties in obtaining geographically representative stool samples and expand surveillance to regions lacking adequate laboratory facilities.https://wwwnc.cdc.gov/eid/article/17/1/10-1132_articleRotavirusgenotypingaffinity purificationvirusesIsraelresearch |
| spellingShingle | Lester M. Shulman Ilana Silberstein Jacqueline Alfandari Ella Mendelson Genotyping Rotavirus RNA from Archived Rotavirus-Positive Rapid Test Strips Emerging Infectious Diseases Rotavirus genotyping affinity purification viruses Israel research |
| title | Genotyping Rotavirus RNA from Archived Rotavirus-Positive Rapid Test Strips |
| title_full | Genotyping Rotavirus RNA from Archived Rotavirus-Positive Rapid Test Strips |
| title_fullStr | Genotyping Rotavirus RNA from Archived Rotavirus-Positive Rapid Test Strips |
| title_full_unstemmed | Genotyping Rotavirus RNA from Archived Rotavirus-Positive Rapid Test Strips |
| title_short | Genotyping Rotavirus RNA from Archived Rotavirus-Positive Rapid Test Strips |
| title_sort | genotyping rotavirus rna from archived rotavirus positive rapid test strips |
| topic | Rotavirus genotyping affinity purification viruses Israel research |
| url | https://wwwnc.cdc.gov/eid/article/17/1/10-1132_article |
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