Transcriptional profiling of ectoderm specification to keratinocyte fate in human embryonic stem cells.
In recent years, several studies have shed light into the processes that regulate epidermal specification and homeostasis. We previously showed that a broad-spectrum γ-secretase inhibitor DAPT promoted early keratinocyte specification in human embryonic stem cells triggered to undergo ectoderm speci...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4388500?pdf=render |
| _version_ | 1818013637316444160 |
|---|---|
| author | Ana Mafalda Baptista Tadeu Samantha Lin Lin Hou Lisa Chung Mei Zhong Hongyu Zhao Valerie Horsley |
| author_facet | Ana Mafalda Baptista Tadeu Samantha Lin Lin Hou Lisa Chung Mei Zhong Hongyu Zhao Valerie Horsley |
| author_sort | Ana Mafalda Baptista Tadeu |
| collection | DOAJ |
| description | In recent years, several studies have shed light into the processes that regulate epidermal specification and homeostasis. We previously showed that a broad-spectrum γ-secretase inhibitor DAPT promoted early keratinocyte specification in human embryonic stem cells triggered to undergo ectoderm specification. Here, we show that DAPT accelerates human embryonic stem cell differentiation and induces expression of the ectoderm protein AP2. Furthermore, we utilize RNA sequencing to identify several candidate regulators of ectoderm specification including those involved in epithelial and epidermal development in human embryonic stem cells. Genes associated with transcriptional regulation and growth factor activity are significantly enriched upon DAPT treatment during specification of human embryonic stem cells to the ectoderm lineage. The human ectoderm cell signature identified in this study contains several genes expressed in ectodermal and epithelial tissues. Importantly, these genes are also associated with skin disorders and ectodermal defects, providing a platform for understanding the biology of human epidermal keratinocyte development under diseased and homeostatic conditions. |
| first_indexed | 2024-04-14T06:35:45Z |
| format | Article |
| id | doaj.art-b69b515f2a5041a187f6851dd04c8c1d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T06:35:45Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b69b515f2a5041a187f6851dd04c8c1d2022-12-22T02:07:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01104e012249310.1371/journal.pone.0122493Transcriptional profiling of ectoderm specification to keratinocyte fate in human embryonic stem cells.Ana Mafalda Baptista TadeuSamantha LinLin HouLisa ChungMei ZhongHongyu ZhaoValerie HorsleyIn recent years, several studies have shed light into the processes that regulate epidermal specification and homeostasis. We previously showed that a broad-spectrum γ-secretase inhibitor DAPT promoted early keratinocyte specification in human embryonic stem cells triggered to undergo ectoderm specification. Here, we show that DAPT accelerates human embryonic stem cell differentiation and induces expression of the ectoderm protein AP2. Furthermore, we utilize RNA sequencing to identify several candidate regulators of ectoderm specification including those involved in epithelial and epidermal development in human embryonic stem cells. Genes associated with transcriptional regulation and growth factor activity are significantly enriched upon DAPT treatment during specification of human embryonic stem cells to the ectoderm lineage. The human ectoderm cell signature identified in this study contains several genes expressed in ectodermal and epithelial tissues. Importantly, these genes are also associated with skin disorders and ectodermal defects, providing a platform for understanding the biology of human epidermal keratinocyte development under diseased and homeostatic conditions.http://europepmc.org/articles/PMC4388500?pdf=render |
| spellingShingle | Ana Mafalda Baptista Tadeu Samantha Lin Lin Hou Lisa Chung Mei Zhong Hongyu Zhao Valerie Horsley Transcriptional profiling of ectoderm specification to keratinocyte fate in human embryonic stem cells. PLoS ONE |
| title | Transcriptional profiling of ectoderm specification to keratinocyte fate in human embryonic stem cells. |
| title_full | Transcriptional profiling of ectoderm specification to keratinocyte fate in human embryonic stem cells. |
| title_fullStr | Transcriptional profiling of ectoderm specification to keratinocyte fate in human embryonic stem cells. |
| title_full_unstemmed | Transcriptional profiling of ectoderm specification to keratinocyte fate in human embryonic stem cells. |
| title_short | Transcriptional profiling of ectoderm specification to keratinocyte fate in human embryonic stem cells. |
| title_sort | transcriptional profiling of ectoderm specification to keratinocyte fate in human embryonic stem cells |
| url | http://europepmc.org/articles/PMC4388500?pdf=render |
| work_keys_str_mv | AT anamafaldabaptistatadeu transcriptionalprofilingofectodermspecificationtokeratinocytefateinhumanembryonicstemcells AT samanthalin transcriptionalprofilingofectodermspecificationtokeratinocytefateinhumanembryonicstemcells AT linhou transcriptionalprofilingofectodermspecificationtokeratinocytefateinhumanembryonicstemcells AT lisachung transcriptionalprofilingofectodermspecificationtokeratinocytefateinhumanembryonicstemcells AT meizhong transcriptionalprofilingofectodermspecificationtokeratinocytefateinhumanembryonicstemcells AT hongyuzhao transcriptionalprofilingofectodermspecificationtokeratinocytefateinhumanembryonicstemcells AT valeriehorsley transcriptionalprofilingofectodermspecificationtokeratinocytefateinhumanembryonicstemcells |