Utilization of Aminoguanidine Prevents Cytotoxic Effects of Semen
Studies of human semen in cell or tissue culture are hampered by the high cytotoxic activity of this body fluid. The components responsible for the cell damaging activity of semen are amine oxidases, which convert abundant polyamines, such as spermine or spermidine in seminal plasma into toxic inter...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/15/8563 |
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| author | Mirja Harms Pascal von Maltitz Rüdiger Groß Benjamin Mayer Miriam Deniz Janis Müller Jan Münch |
| author_facet | Mirja Harms Pascal von Maltitz Rüdiger Groß Benjamin Mayer Miriam Deniz Janis Müller Jan Münch |
| author_sort | Mirja Harms |
| collection | DOAJ |
| description | Studies of human semen in cell or tissue culture are hampered by the high cytotoxic activity of this body fluid. The components responsible for the cell damaging activity of semen are amine oxidases, which convert abundant polyamines, such as spermine or spermidine in seminal plasma into toxic intermediates. Amine oxidases are naturally present at low concentrations in seminal plasma and at high concentrations in fetal calf serum, a commonly used cell culture supplement. Here, we show that, in the presence of fetal calf serum, seminal plasma, as well as the polyamines spermine and spermidine, are highly cytotoxic to immortalized cells, primary blood mononuclear cells, and vaginal tissue. Thus, experiments investigating the effect of polyamines and seminal plasma on cellular functions should be performed with great caution, considering the confounding cytotoxic effects. The addition of the amine oxidase inhibitor aminoguanidine to fetal calf serum and/or the utilization of serum-free medium greatly reduced this serum-induced cytotoxicity of polyamines and seminal plasma in cell lines, primary cells, and tissues and, thus, should be implemented in all future studies analyzing the role of polyamines and semen on cellular functions. |
| first_indexed | 2024-03-09T12:32:04Z |
| format | Article |
| id | doaj.art-b69c7b5a93ff4099984213231df1e61c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T12:32:04Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b69c7b5a93ff4099984213231df1e61c2023-11-30T22:29:18ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012315856310.3390/ijms23158563Utilization of Aminoguanidine Prevents Cytotoxic Effects of SemenMirja Harms0Pascal von Maltitz1Rüdiger Groß2Benjamin Mayer3Miriam Deniz4Janis Müller5Jan Münch6Institute of Molecular Virology, University Ulm Medical Center, 89081 Ulm, GermanyInstitute of Molecular Virology, University Ulm Medical Center, 89081 Ulm, GermanyInstitute of Molecular Virology, University Ulm Medical Center, 89081 Ulm, GermanyInstitute for Epidemiology and Medical Biometry, Ulm University, 89075 Ulm, GermanyDepartment of Gynecology and Obstetrics, Ulm University Hospital, 89075 Ulm, GermanyInstitute of Molecular Virology, University Ulm Medical Center, 89081 Ulm, GermanyInstitute of Molecular Virology, University Ulm Medical Center, 89081 Ulm, GermanyStudies of human semen in cell or tissue culture are hampered by the high cytotoxic activity of this body fluid. The components responsible for the cell damaging activity of semen are amine oxidases, which convert abundant polyamines, such as spermine or spermidine in seminal plasma into toxic intermediates. Amine oxidases are naturally present at low concentrations in seminal plasma and at high concentrations in fetal calf serum, a commonly used cell culture supplement. Here, we show that, in the presence of fetal calf serum, seminal plasma, as well as the polyamines spermine and spermidine, are highly cytotoxic to immortalized cells, primary blood mononuclear cells, and vaginal tissue. Thus, experiments investigating the effect of polyamines and seminal plasma on cellular functions should be performed with great caution, considering the confounding cytotoxic effects. The addition of the amine oxidase inhibitor aminoguanidine to fetal calf serum and/or the utilization of serum-free medium greatly reduced this serum-induced cytotoxicity of polyamines and seminal plasma in cell lines, primary cells, and tissues and, thus, should be implemented in all future studies analyzing the role of polyamines and semen on cellular functions.https://www.mdpi.com/1422-0067/23/15/8563seminal fluidcytotoxicitypolyaminesspermine |
| spellingShingle | Mirja Harms Pascal von Maltitz Rüdiger Groß Benjamin Mayer Miriam Deniz Janis Müller Jan Münch Utilization of Aminoguanidine Prevents Cytotoxic Effects of Semen International Journal of Molecular Sciences seminal fluid cytotoxicity polyamines spermine |
| title | Utilization of Aminoguanidine Prevents Cytotoxic Effects of Semen |
| title_full | Utilization of Aminoguanidine Prevents Cytotoxic Effects of Semen |
| title_fullStr | Utilization of Aminoguanidine Prevents Cytotoxic Effects of Semen |
| title_full_unstemmed | Utilization of Aminoguanidine Prevents Cytotoxic Effects of Semen |
| title_short | Utilization of Aminoguanidine Prevents Cytotoxic Effects of Semen |
| title_sort | utilization of aminoguanidine prevents cytotoxic effects of semen |
| topic | seminal fluid cytotoxicity polyamines spermine |
| url | https://www.mdpi.com/1422-0067/23/15/8563 |
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