Polyphenolic Compounds from <i>Lespedeza bicolor</i> Protect Neuronal Cells from Oxidative Stress
Pterocarpans and related polyphenolics are known as promising neuroprotective agents. We used models of rotenone-, paraquat-, and 6-hydroxydopamine-induced neurotoxicity to study the neuroprotective activity of polyphenolic compounds from <i>Lespedeza bicolor</i> and their effects on mit...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/4/709 |
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| author | Darya V. Tarbeeva Evgeny A. Pislyagin Ekaterina S. Menchinskaya Dmitrii V. Berdyshev Anatoliy I. Kalinovskiy Valeria P. Grigorchuk Natalia P. Mishchenko Dmitry L. Aminin Sergey A. Fedoreyev |
| author_facet | Darya V. Tarbeeva Evgeny A. Pislyagin Ekaterina S. Menchinskaya Dmitrii V. Berdyshev Anatoliy I. Kalinovskiy Valeria P. Grigorchuk Natalia P. Mishchenko Dmitry L. Aminin Sergey A. Fedoreyev |
| author_sort | Darya V. Tarbeeva |
| collection | DOAJ |
| description | Pterocarpans and related polyphenolics are known as promising neuroprotective agents. We used models of rotenone-, paraquat-, and 6-hydroxydopamine-induced neurotoxicity to study the neuroprotective activity of polyphenolic compounds from <i>Lespedeza bicolor</i> and their effects on mitochondrial membrane potential. We isolated 11 polyphenolic compounds: a novel coumestan lespebicoumestan A (<b>10</b>) and a novel stilbenoid 5’-isoprenylbicoloketon (<b>11</b>) as well as three previously known pterocarpans, two pterocarpens, one coumestan, one stilbenoid, and a dimeric flavonoid. Pterocarpans <b>3</b> and <b>6</b>, stilbenoid <b>5</b>, and dimeric flavonoid <b>8</b> significantly increased the percentage of living cells after treatment with paraquat (PQ), but only pterocarpan <b>6</b> slightly decreased the ROS level in PQ-treated cells. Pterocarpan <b>3</b> and stilbenoid <b>5</b> were shown to effectively increase mitochondrial membrane potential in PQ-treated cells. We showed that pterocarpans <b>2</b> and <b>3</b>, containing a 3’-methyl-3’-isohexenylpyran ring; pterocarpens <b>4</b> and <b>9</b>, with a double bond between C-6a and C-11a; and coumestan <b>10</b> significantly increased the percentage of living cells by decreasing ROS levels in 6-OHDA-treated cells, which is in accordance with their rather high activity in DPPH<sup>•</sup> and FRAP tests. Compounds <b>9</b> and <b>10</b> effectively increased the percentage of living cells after treatment with rotenone but did not significantly decrease ROS levels. |
| first_indexed | 2024-03-09T11:14:16Z |
| format | Article |
| id | doaj.art-b69c82f9b7d14537bb868cb3da10970d |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T11:14:16Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-b69c82f9b7d14537bb868cb3da10970d2023-12-01T00:35:32ZengMDPI AGAntioxidants2076-39212022-04-0111470910.3390/antiox11040709Polyphenolic Compounds from <i>Lespedeza bicolor</i> Protect Neuronal Cells from Oxidative StressDarya V. Tarbeeva0Evgeny A. Pislyagin1Ekaterina S. Menchinskaya2Dmitrii V. Berdyshev3Anatoliy I. Kalinovskiy4Valeria P. Grigorchuk5Natalia P. Mishchenko6Dmitry L. Aminin7Sergey A. Fedoreyev8G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Science, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Science, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Science, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Science, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Science, 690022 Vladivostok, RussiaFederal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Science, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Science, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Science, 690022 Vladivostok, RussiaPterocarpans and related polyphenolics are known as promising neuroprotective agents. We used models of rotenone-, paraquat-, and 6-hydroxydopamine-induced neurotoxicity to study the neuroprotective activity of polyphenolic compounds from <i>Lespedeza bicolor</i> and their effects on mitochondrial membrane potential. We isolated 11 polyphenolic compounds: a novel coumestan lespebicoumestan A (<b>10</b>) and a novel stilbenoid 5’-isoprenylbicoloketon (<b>11</b>) as well as three previously known pterocarpans, two pterocarpens, one coumestan, one stilbenoid, and a dimeric flavonoid. Pterocarpans <b>3</b> and <b>6</b>, stilbenoid <b>5</b>, and dimeric flavonoid <b>8</b> significantly increased the percentage of living cells after treatment with paraquat (PQ), but only pterocarpan <b>6</b> slightly decreased the ROS level in PQ-treated cells. Pterocarpan <b>3</b> and stilbenoid <b>5</b> were shown to effectively increase mitochondrial membrane potential in PQ-treated cells. We showed that pterocarpans <b>2</b> and <b>3</b>, containing a 3’-methyl-3’-isohexenylpyran ring; pterocarpens <b>4</b> and <b>9</b>, with a double bond between C-6a and C-11a; and coumestan <b>10</b> significantly increased the percentage of living cells by decreasing ROS levels in 6-OHDA-treated cells, which is in accordance with their rather high activity in DPPH<sup>•</sup> and FRAP tests. Compounds <b>9</b> and <b>10</b> effectively increased the percentage of living cells after treatment with rotenone but did not significantly decrease ROS levels.https://www.mdpi.com/2076-3921/11/4/709polyphenolic compoundspterocarpanscoumestansParkinson’s diseaseoxidative stressneuronal damage |
| spellingShingle | Darya V. Tarbeeva Evgeny A. Pislyagin Ekaterina S. Menchinskaya Dmitrii V. Berdyshev Anatoliy I. Kalinovskiy Valeria P. Grigorchuk Natalia P. Mishchenko Dmitry L. Aminin Sergey A. Fedoreyev Polyphenolic Compounds from <i>Lespedeza bicolor</i> Protect Neuronal Cells from Oxidative Stress Antioxidants polyphenolic compounds pterocarpans coumestans Parkinson’s disease oxidative stress neuronal damage |
| title | Polyphenolic Compounds from <i>Lespedeza bicolor</i> Protect Neuronal Cells from Oxidative Stress |
| title_full | Polyphenolic Compounds from <i>Lespedeza bicolor</i> Protect Neuronal Cells from Oxidative Stress |
| title_fullStr | Polyphenolic Compounds from <i>Lespedeza bicolor</i> Protect Neuronal Cells from Oxidative Stress |
| title_full_unstemmed | Polyphenolic Compounds from <i>Lespedeza bicolor</i> Protect Neuronal Cells from Oxidative Stress |
| title_short | Polyphenolic Compounds from <i>Lespedeza bicolor</i> Protect Neuronal Cells from Oxidative Stress |
| title_sort | polyphenolic compounds from i lespedeza bicolor i protect neuronal cells from oxidative stress |
| topic | polyphenolic compounds pterocarpans coumestans Parkinson’s disease oxidative stress neuronal damage |
| url | https://www.mdpi.com/2076-3921/11/4/709 |
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