Validation of an RP-HPLC Method for the Determination of Asenapine Maleate in Dissolution Media and Application to Study In Vitro Release from Co-Crystals
Asenapine maleate is an antipsychotic drug that is indicated in the treatment of schizophrenia and bipolar disorders. It has low aqueous solubility and high permeability (Class II drug) and undergoes an extensive first pass effect. These problems result in low oral bioavailability (<2%). To enhan...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Scientia Pharmaceutica |
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| Online Access: | https://www.mdpi.com/2218-0532/89/1/14 |
| _version_ | 1797542206288953344 |
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| author | Suhair S. Al-Nimry Mai S. Khanfar |
| author_facet | Suhair S. Al-Nimry Mai S. Khanfar |
| author_sort | Suhair S. Al-Nimry |
| collection | DOAJ |
| description | Asenapine maleate is an antipsychotic drug that is indicated in the treatment of schizophrenia and bipolar disorders. It has low aqueous solubility and high permeability (Class II drug) and undergoes an extensive first pass effect. These problems result in low oral bioavailability (<2%). To enhance its solubility/dissolution rate and hence bioavailability, co-crystals using different co-formers in different ratios were prepared and evaluated. To study the in vitro dissolution of the drug from these co-crystals into phosphate buffer (pH 6.8), an RP-HPLC method was developed and validated according to the ICH Q2R1 guidelines. The method was linear in the range 0.1–14 µg/mL (R > 0.9998) and accurate and precise. An ANOVA test indicated that calibration curves run on different days did not differ significantly. It was sensitive (lower limit of quantitation (LLOQ) = 25.03 ng/mL), specific (the co-formers did not interfere with the determination of the drug), and robust to small changes in the mobile phase (pH, composition, and flow rate). The in vitro release of asenapine maleate from the co-crystals and the physical mixture was much enhanced when compared to the in vitro dissolution of the unprocessed drug. In conclusion, the developed and validated RP-HPLC method met the acceptance criteria and was applied successfully in evaluating the in vitro release of the drug. |
| first_indexed | 2024-03-10T13:26:23Z |
| format | Article |
| id | doaj.art-b69e72d3d6554e8eb6ca656b49410f4d |
| institution | Directory Open Access Journal |
| issn | 0036-8709 2218-0532 |
| language | English |
| last_indexed | 2024-03-10T13:26:23Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Scientia Pharmaceutica |
| spelling | doaj.art-b69e72d3d6554e8eb6ca656b49410f4d2023-11-21T09:36:15ZengMDPI AGScientia Pharmaceutica0036-87092218-05322021-03-018911410.3390/scipharm89010014Validation of an RP-HPLC Method for the Determination of Asenapine Maleate in Dissolution Media and Application to Study In Vitro Release from Co-CrystalsSuhair S. Al-Nimry0Mai S. Khanfar1Department of Pharmaceutical Technology, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, JordanDepartment of Pharmaceutical Technology, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, JordanAsenapine maleate is an antipsychotic drug that is indicated in the treatment of schizophrenia and bipolar disorders. It has low aqueous solubility and high permeability (Class II drug) and undergoes an extensive first pass effect. These problems result in low oral bioavailability (<2%). To enhance its solubility/dissolution rate and hence bioavailability, co-crystals using different co-formers in different ratios were prepared and evaluated. To study the in vitro dissolution of the drug from these co-crystals into phosphate buffer (pH 6.8), an RP-HPLC method was developed and validated according to the ICH Q2R1 guidelines. The method was linear in the range 0.1–14 µg/mL (R > 0.9998) and accurate and precise. An ANOVA test indicated that calibration curves run on different days did not differ significantly. It was sensitive (lower limit of quantitation (LLOQ) = 25.03 ng/mL), specific (the co-formers did not interfere with the determination of the drug), and robust to small changes in the mobile phase (pH, composition, and flow rate). The in vitro release of asenapine maleate from the co-crystals and the physical mixture was much enhanced when compared to the in vitro dissolution of the unprocessed drug. In conclusion, the developed and validated RP-HPLC method met the acceptance criteria and was applied successfully in evaluating the in vitro release of the drug.https://www.mdpi.com/2218-0532/89/1/14asenapine maleateRP-HPLC methodvalidationco-crystalsapplicationin vitro release |
| spellingShingle | Suhair S. Al-Nimry Mai S. Khanfar Validation of an RP-HPLC Method for the Determination of Asenapine Maleate in Dissolution Media and Application to Study In Vitro Release from Co-Crystals Scientia Pharmaceutica asenapine maleate RP-HPLC method validation co-crystals application in vitro release |
| title | Validation of an RP-HPLC Method for the Determination of Asenapine Maleate in Dissolution Media and Application to Study In Vitro Release from Co-Crystals |
| title_full | Validation of an RP-HPLC Method for the Determination of Asenapine Maleate in Dissolution Media and Application to Study In Vitro Release from Co-Crystals |
| title_fullStr | Validation of an RP-HPLC Method for the Determination of Asenapine Maleate in Dissolution Media and Application to Study In Vitro Release from Co-Crystals |
| title_full_unstemmed | Validation of an RP-HPLC Method for the Determination of Asenapine Maleate in Dissolution Media and Application to Study In Vitro Release from Co-Crystals |
| title_short | Validation of an RP-HPLC Method for the Determination of Asenapine Maleate in Dissolution Media and Application to Study In Vitro Release from Co-Crystals |
| title_sort | validation of an rp hplc method for the determination of asenapine maleate in dissolution media and application to study in vitro release from co crystals |
| topic | asenapine maleate RP-HPLC method validation co-crystals application in vitro release |
| url | https://www.mdpi.com/2218-0532/89/1/14 |
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