Consumption of antimicrobial manuka honey does not significantly perturb the microbiota in the hind gut of mice
The aim of this study was to test the hypothesis that consuming manuka honey, which contains antimicrobial methylglyoxal, may affect the gut microbiota. We undertook a mouse feeding study to investigate whether dietary manuka honey supplementation altered microbial numbers and their production of or...
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| Format: | Article |
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PeerJ Inc.
2016-12-01
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| Online Access: | https://peerj.com/articles/2787.pdf |
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| author | Doug Rosendale Christine A. Butts Cloe Erika de Guzman Ian S. Maddox Sheridan Martell Lynn McIntyre Margot A. Skinner Hannah Dinnan Juliet Ansell |
| author_facet | Doug Rosendale Christine A. Butts Cloe Erika de Guzman Ian S. Maddox Sheridan Martell Lynn McIntyre Margot A. Skinner Hannah Dinnan Juliet Ansell |
| author_sort | Doug Rosendale |
| collection | DOAJ |
| description | The aim of this study was to test the hypothesis that consuming manuka honey, which contains antimicrobial methylglyoxal, may affect the gut microbiota. We undertook a mouse feeding study to investigate whether dietary manuka honey supplementation altered microbial numbers and their production of organic acid products from carbohydrate fermentation, which are markers of gut microbiota function. The caecum of C57BL/6 mice fed a diet supplemented with antimicrobial UMF® 20+ manuka honey at 2.2 g/kg animal did not show any significantly changed concentrations of microbial short chain fatty acids as measured by gas chromatography, except for increased formate and lowered succinate organic acid concentrations, compared to mice fed a control diet. There was no change in succinate-producing Bacteroidetes numbers, or honey-utilising Bifidobacteria, nor any other microbes measured by real time quantitative PCR. These results suggest that, despite the antimicrobial activity of the original honey, consumption of manuka honey only mildly affects substrate metabolism by the gut microbiota. |
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| format | Article |
| id | doaj.art-b69f922f9fcc434dacdbeff8dbd27eb7 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:32:19Z |
| publishDate | 2016-12-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-b69f922f9fcc434dacdbeff8dbd27eb72023-12-03T11:03:46ZengPeerJ Inc.PeerJ2167-83592016-12-014e278710.7717/peerj.2787Consumption of antimicrobial manuka honey does not significantly perturb the microbiota in the hind gut of miceDoug Rosendale0Christine A. Butts1Cloe Erika de Guzman2Ian S. Maddox3Sheridan Martell4Lynn McIntyre5Margot A. Skinner6Hannah Dinnan7Juliet Ansell8Food, Nutrition & Health Group, The New Zealand Institute for Plant & Food Research Limited, Palmerston North, New ZealandFood, Nutrition & Health Group, The New Zealand Institute for Plant & Food Research Limited, Palmerston North, New ZealandTranslational Genomics Group, Institute of Health and Biomedical Innovation, Brisbane, Queensland, AustraliaMassey University, College of Sciences, Auckland, New ZealandFood, Nutrition & Health Group, The New Zealand Institute for Plant & Food Research Limited, Palmerston North, New ZealandMassey University, College of Sciences, Auckland, New ZealandFood Science, School of Chemical Science, The University of Auckland, Auckland, New ZealandFood, Nutrition & Health Group, The New Zealand Institute for Plant & Food Research Limited, Palmerston North, New ZealandFood, Nutrition & Health Group, The New Zealand Institute for Plant & Food Research Limited, Palmerston North, New ZealandThe aim of this study was to test the hypothesis that consuming manuka honey, which contains antimicrobial methylglyoxal, may affect the gut microbiota. We undertook a mouse feeding study to investigate whether dietary manuka honey supplementation altered microbial numbers and their production of organic acid products from carbohydrate fermentation, which are markers of gut microbiota function. The caecum of C57BL/6 mice fed a diet supplemented with antimicrobial UMF® 20+ manuka honey at 2.2 g/kg animal did not show any significantly changed concentrations of microbial short chain fatty acids as measured by gas chromatography, except for increased formate and lowered succinate organic acid concentrations, compared to mice fed a control diet. There was no change in succinate-producing Bacteroidetes numbers, or honey-utilising Bifidobacteria, nor any other microbes measured by real time quantitative PCR. These results suggest that, despite the antimicrobial activity of the original honey, consumption of manuka honey only mildly affects substrate metabolism by the gut microbiota.https://peerj.com/articles/2787.pdfManukaHoneyMetabolismMicrobiotaMethylglyoxalOrganic acids |
| spellingShingle | Doug Rosendale Christine A. Butts Cloe Erika de Guzman Ian S. Maddox Sheridan Martell Lynn McIntyre Margot A. Skinner Hannah Dinnan Juliet Ansell Consumption of antimicrobial manuka honey does not significantly perturb the microbiota in the hind gut of mice PeerJ Manuka Honey Metabolism Microbiota Methylglyoxal Organic acids |
| title | Consumption of antimicrobial manuka honey does not significantly perturb the microbiota in the hind gut of mice |
| title_full | Consumption of antimicrobial manuka honey does not significantly perturb the microbiota in the hind gut of mice |
| title_fullStr | Consumption of antimicrobial manuka honey does not significantly perturb the microbiota in the hind gut of mice |
| title_full_unstemmed | Consumption of antimicrobial manuka honey does not significantly perturb the microbiota in the hind gut of mice |
| title_short | Consumption of antimicrobial manuka honey does not significantly perturb the microbiota in the hind gut of mice |
| title_sort | consumption of antimicrobial manuka honey does not significantly perturb the microbiota in the hind gut of mice |
| topic | Manuka Honey Metabolism Microbiota Methylglyoxal Organic acids |
| url | https://peerj.com/articles/2787.pdf |
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