Multi-Approach Characterization of Novel Pyrene-Degrading <i>Mycolicibacterium austroafricanum</i> Isolates Lacking <i>nid</i> Genes
Polycyclic aromatic hydrocarbons (PAHs) are chemical compounds that are widespread in the environment, arising from the incomplete combustion of organic material, as well as from human activities involving petrol exploitation, petrochemical industrial waste, gas stations, and environmental disasters...
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MDPI AG
2023-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/6/1413 |
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| author | Natalia Maria Silva Camila Lopes Romagnoli Caio Rafael do Nascimento Santiago João Paulo Amorim de Lacerda Sylvia Cardoso Leão Luciano Antonio Digiampietri Cristina Viana-Niero |
| author_facet | Natalia Maria Silva Camila Lopes Romagnoli Caio Rafael do Nascimento Santiago João Paulo Amorim de Lacerda Sylvia Cardoso Leão Luciano Antonio Digiampietri Cristina Viana-Niero |
| author_sort | Natalia Maria Silva |
| collection | DOAJ |
| description | Polycyclic aromatic hydrocarbons (PAHs) are chemical compounds that are widespread in the environment, arising from the incomplete combustion of organic material, as well as from human activities involving petrol exploitation, petrochemical industrial waste, gas stations, and environmental disasters. PAHs of high molecular weight, such as pyrene, have carcinogenic and mutagenic effects and are considered pollutants. The microbial degradation of PAHs occurs through the action of multiple dioxygenase genes (<i>nid</i>), which are localized in genomic island denominate region A, and cytochrome P450 monooxygenases genes (<i>cyp</i>) dispersed in the bacterial genome. This study evaluated pyrene degradation by five isolates of <i>Mycolicibacterium austroafricanum</i> using 2,6-dichlorophenol indophenol (DCPIP assay), gas chromatography/mass spectrometry (CG/MS), and genomic analyses. Two isolates (MYC038 and MYC040) exhibited pyrene degradation indexes of 96% and 88%, respectively, over a seven-day incubation period. Interestingly, the genomic analyses showed that the isolates do not have <i>nid</i> genes, which are involved in PAH biodegradation, despite their ability to degrade pyrene, suggesting that degradation may occur due to the presence of <i>cyp</i>150 genes, or even genes that have not yet been described. To the best of our knowledge, this is the first report of isolates without <i>nid</i> genes demonstrating the ability to degrade pyrene. |
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| language | English |
| last_indexed | 2024-03-11T02:08:33Z |
| publishDate | 2023-05-01 |
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| series | Microorganisms |
| spelling | doaj.art-b6a16978c31f4c9ca0fd2f6d05439efb2023-11-18T11:41:33ZengMDPI AGMicroorganisms2076-26072023-05-01116141310.3390/microorganisms11061413Multi-Approach Characterization of Novel Pyrene-Degrading <i>Mycolicibacterium austroafricanum</i> Isolates Lacking <i>nid</i> GenesNatalia Maria Silva0Camila Lopes Romagnoli1Caio Rafael do Nascimento Santiago2João Paulo Amorim de Lacerda3Sylvia Cardoso Leão4Luciano Antonio Digiampietri5Cristina Viana-Niero6Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo 04023-901, BrazilDepartment of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo 04023-901, BrazilSchool of Arts, Sciences and Humanities, University of São Paulo, São Paulo 03828-000, BrazilLaboratory of Chemistry and Manufactured Products, Institute of Technological Research, São Paulo 05508-901, BrazilDepartment of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo 04023-901, BrazilSchool of Arts, Sciences and Humanities, University of São Paulo, São Paulo 03828-000, BrazilDepartment of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo 04023-901, BrazilPolycyclic aromatic hydrocarbons (PAHs) are chemical compounds that are widespread in the environment, arising from the incomplete combustion of organic material, as well as from human activities involving petrol exploitation, petrochemical industrial waste, gas stations, and environmental disasters. PAHs of high molecular weight, such as pyrene, have carcinogenic and mutagenic effects and are considered pollutants. The microbial degradation of PAHs occurs through the action of multiple dioxygenase genes (<i>nid</i>), which are localized in genomic island denominate region A, and cytochrome P450 monooxygenases genes (<i>cyp</i>) dispersed in the bacterial genome. This study evaluated pyrene degradation by five isolates of <i>Mycolicibacterium austroafricanum</i> using 2,6-dichlorophenol indophenol (DCPIP assay), gas chromatography/mass spectrometry (CG/MS), and genomic analyses. Two isolates (MYC038 and MYC040) exhibited pyrene degradation indexes of 96% and 88%, respectively, over a seven-day incubation period. Interestingly, the genomic analyses showed that the isolates do not have <i>nid</i> genes, which are involved in PAH biodegradation, despite their ability to degrade pyrene, suggesting that degradation may occur due to the presence of <i>cyp</i>150 genes, or even genes that have not yet been described. To the best of our knowledge, this is the first report of isolates without <i>nid</i> genes demonstrating the ability to degrade pyrene.https://www.mdpi.com/2076-2607/11/6/1413genomic analysispolycyclic aromatic hydrocarbongas chromatography/mass spectrometry |
| spellingShingle | Natalia Maria Silva Camila Lopes Romagnoli Caio Rafael do Nascimento Santiago João Paulo Amorim de Lacerda Sylvia Cardoso Leão Luciano Antonio Digiampietri Cristina Viana-Niero Multi-Approach Characterization of Novel Pyrene-Degrading <i>Mycolicibacterium austroafricanum</i> Isolates Lacking <i>nid</i> Genes Microorganisms genomic analysis polycyclic aromatic hydrocarbon gas chromatography/mass spectrometry |
| title | Multi-Approach Characterization of Novel Pyrene-Degrading <i>Mycolicibacterium austroafricanum</i> Isolates Lacking <i>nid</i> Genes |
| title_full | Multi-Approach Characterization of Novel Pyrene-Degrading <i>Mycolicibacterium austroafricanum</i> Isolates Lacking <i>nid</i> Genes |
| title_fullStr | Multi-Approach Characterization of Novel Pyrene-Degrading <i>Mycolicibacterium austroafricanum</i> Isolates Lacking <i>nid</i> Genes |
| title_full_unstemmed | Multi-Approach Characterization of Novel Pyrene-Degrading <i>Mycolicibacterium austroafricanum</i> Isolates Lacking <i>nid</i> Genes |
| title_short | Multi-Approach Characterization of Novel Pyrene-Degrading <i>Mycolicibacterium austroafricanum</i> Isolates Lacking <i>nid</i> Genes |
| title_sort | multi approach characterization of novel pyrene degrading i mycolicibacterium austroafricanum i isolates lacking i nid i genes |
| topic | genomic analysis polycyclic aromatic hydrocarbon gas chromatography/mass spectrometry |
| url | https://www.mdpi.com/2076-2607/11/6/1413 |
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