The epithelial-specific ER stress sensor ERN2/IRE1β enables host-microbiota crosstalk to affect colon goblet cell development
Epithelial cells lining mucosal surfaces of the gastrointestinal and respiratory tracts uniquely express ERN2/IRE1β, a paralogue of the most evolutionarily conserved endoplasmic reticulum stress sensor, ERN1/IRE1α. How ERN2 functions at the host-environment interface and why a second paralogue evolv...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Clinical Investigation
2022-09-01
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| Series: | The Journal of Clinical Investigation |
| Subjects: | |
| Online Access: | https://doi.org/10.1172/JCI153519 |
| _version_ | 1797634614740647936 |
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| author | Michael J. Grey Heidi De Luca Doyle V. Ward Irini A.M. Kreulen Katlynn Bugda Gwilt Sage E. Foley Jay R. Thiagarajah Beth A. McCormick Jerrold R. Turner Wayne I. Lencer |
| author_facet | Michael J. Grey Heidi De Luca Doyle V. Ward Irini A.M. Kreulen Katlynn Bugda Gwilt Sage E. Foley Jay R. Thiagarajah Beth A. McCormick Jerrold R. Turner Wayne I. Lencer |
| author_sort | Michael J. Grey |
| collection | DOAJ |
| description | Epithelial cells lining mucosal surfaces of the gastrointestinal and respiratory tracts uniquely express ERN2/IRE1β, a paralogue of the most evolutionarily conserved endoplasmic reticulum stress sensor, ERN1/IRE1α. How ERN2 functions at the host-environment interface and why a second paralogue evolved remain incompletely understood. Using conventionally raised and germ-free Ern2–/– mice, we found that ERN2 was required for microbiota-induced goblet cell maturation and mucus barrier assembly in the colon. This occurred only after colonization of the alimentary tract with normal gut microflora, which induced Ern2 expression. ERN2 acted by splicing Xbp1 mRNA to expand ER function and prevent ER stress in goblet cells. Although ERN1 can also splice Xbp1 mRNA, it did not act redundantly to ERN2 in this context. By regulating assembly of the colon mucus layer, ERN2 further shaped the composition of the gut microbiota. Mice lacking Ern2 had a dysbiotic microbial community that failed to induce goblet cell development and increased susceptibility to colitis when transferred into germ-free WT mice. These results show that ERN2 evolved at mucosal surfaces to mediate crosstalk between gut microbes and the colonic epithelium required for normal homeostasis and host defense. |
| first_indexed | 2024-03-11T12:10:01Z |
| format | Article |
| id | doaj.art-b6a3451037ac41be97dfd02c6d12fa5c |
| institution | Directory Open Access Journal |
| issn | 1558-8238 |
| language | English |
| last_indexed | 2024-03-11T12:10:01Z |
| publishDate | 2022-09-01 |
| publisher | American Society for Clinical Investigation |
| record_format | Article |
| series | The Journal of Clinical Investigation |
| spelling | doaj.art-b6a3451037ac41be97dfd02c6d12fa5c2023-11-07T16:19:17ZengAmerican Society for Clinical InvestigationThe Journal of Clinical Investigation1558-82382022-09-0113217The epithelial-specific ER stress sensor ERN2/IRE1β enables host-microbiota crosstalk to affect colon goblet cell developmentMichael J. GreyHeidi De LucaDoyle V. WardIrini A.M. KreulenKatlynn Bugda GwiltSage E. FoleyJay R. ThiagarajahBeth A. McCormickJerrold R. TurnerWayne I. LencerEpithelial cells lining mucosal surfaces of the gastrointestinal and respiratory tracts uniquely express ERN2/IRE1β, a paralogue of the most evolutionarily conserved endoplasmic reticulum stress sensor, ERN1/IRE1α. How ERN2 functions at the host-environment interface and why a second paralogue evolved remain incompletely understood. Using conventionally raised and germ-free Ern2–/– mice, we found that ERN2 was required for microbiota-induced goblet cell maturation and mucus barrier assembly in the colon. This occurred only after colonization of the alimentary tract with normal gut microflora, which induced Ern2 expression. ERN2 acted by splicing Xbp1 mRNA to expand ER function and prevent ER stress in goblet cells. Although ERN1 can also splice Xbp1 mRNA, it did not act redundantly to ERN2 in this context. By regulating assembly of the colon mucus layer, ERN2 further shaped the composition of the gut microbiota. Mice lacking Ern2 had a dysbiotic microbial community that failed to induce goblet cell development and increased susceptibility to colitis when transferred into germ-free WT mice. These results show that ERN2 evolved at mucosal surfaces to mediate crosstalk between gut microbes and the colonic epithelium required for normal homeostasis and host defense.https://doi.org/10.1172/JCI153519Gastroenterology |
| spellingShingle | Michael J. Grey Heidi De Luca Doyle V. Ward Irini A.M. Kreulen Katlynn Bugda Gwilt Sage E. Foley Jay R. Thiagarajah Beth A. McCormick Jerrold R. Turner Wayne I. Lencer The epithelial-specific ER stress sensor ERN2/IRE1β enables host-microbiota crosstalk to affect colon goblet cell development The Journal of Clinical Investigation Gastroenterology |
| title | The epithelial-specific ER stress sensor ERN2/IRE1β enables host-microbiota crosstalk to affect colon goblet cell development |
| title_full | The epithelial-specific ER stress sensor ERN2/IRE1β enables host-microbiota crosstalk to affect colon goblet cell development |
| title_fullStr | The epithelial-specific ER stress sensor ERN2/IRE1β enables host-microbiota crosstalk to affect colon goblet cell development |
| title_full_unstemmed | The epithelial-specific ER stress sensor ERN2/IRE1β enables host-microbiota crosstalk to affect colon goblet cell development |
| title_short | The epithelial-specific ER stress sensor ERN2/IRE1β enables host-microbiota crosstalk to affect colon goblet cell development |
| title_sort | epithelial specific er stress sensor ern2 ire1β enables host microbiota crosstalk to affect colon goblet cell development |
| topic | Gastroenterology |
| url | https://doi.org/10.1172/JCI153519 |
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