Molnupiravir Inhibits Porcine Epidemic Diarrhea Virus Infection In Vitro
Porcine epidemic diarrhea virus (PEDV) is a swine coronavirus that is highly infectious and prone to variation. Vaccines derived from traditional PEDV strains provide less protection against PEDV-variant strains. Furthermore; there is a complex diversity of sequences among various PEDV-variant strai...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/6/1317 |
| _version_ | 1827735395734913024 |
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| author | Zi-Xin Huang Shu-Ting Zhou Zhi-Biao Yang Zhe Wang |
| author_facet | Zi-Xin Huang Shu-Ting Zhou Zhi-Biao Yang Zhe Wang |
| author_sort | Zi-Xin Huang |
| collection | DOAJ |
| description | Porcine epidemic diarrhea virus (PEDV) is a swine coronavirus that is highly infectious and prone to variation. Vaccines derived from traditional PEDV strains provide less protection against PEDV-variant strains. Furthermore; there is a complex diversity of sequences among various PEDV-variant strains. Therefore; there is an urgent need to develop alternative antiviral strategies to defend against PEDV. Molnupiravir is a nucleotide analogue that could replace natural nucleosides to restrain viral RNA replication. Our study provided evidence for the dose-dependent inhibition of PEDV replication by molnupiravir in Vero cells. Molnupiravir also exhibited a strong inhibitory effect on viral RNA and protein production. Our results demonstrated that molnupiravir inhibits PEDV RNA-dependent RNA polymerase (RdRp) activity and induces a high frequency of mutations in the PEDV genome. Further studies revealed that molnupiravir can reverse changes in the transcriptome caused by viral infection. In conclusion, our results indicated that molnupiravir has the potential to be an effective treatment for PEDV infection. |
| first_indexed | 2024-03-11T01:50:21Z |
| format | Article |
| id | doaj.art-b6a5c6a99d00458085dd041cac2d46b6 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T01:50:21Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-b6a5c6a99d00458085dd041cac2d46b62023-11-18T13:02:03ZengMDPI AGViruses1999-49152023-06-01156131710.3390/v15061317Molnupiravir Inhibits Porcine Epidemic Diarrhea Virus Infection In VitroZi-Xin Huang0Shu-Ting Zhou1Zhi-Biao Yang2Zhe Wang3Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaPorcine epidemic diarrhea virus (PEDV) is a swine coronavirus that is highly infectious and prone to variation. Vaccines derived from traditional PEDV strains provide less protection against PEDV-variant strains. Furthermore; there is a complex diversity of sequences among various PEDV-variant strains. Therefore; there is an urgent need to develop alternative antiviral strategies to defend against PEDV. Molnupiravir is a nucleotide analogue that could replace natural nucleosides to restrain viral RNA replication. Our study provided evidence for the dose-dependent inhibition of PEDV replication by molnupiravir in Vero cells. Molnupiravir also exhibited a strong inhibitory effect on viral RNA and protein production. Our results demonstrated that molnupiravir inhibits PEDV RNA-dependent RNA polymerase (RdRp) activity and induces a high frequency of mutations in the PEDV genome. Further studies revealed that molnupiravir can reverse changes in the transcriptome caused by viral infection. In conclusion, our results indicated that molnupiravir has the potential to be an effective treatment for PEDV infection.https://www.mdpi.com/1999-4915/15/6/1317PEDVmolnupiravirantiviralRdRp activityRNA-Seq |
| spellingShingle | Zi-Xin Huang Shu-Ting Zhou Zhi-Biao Yang Zhe Wang Molnupiravir Inhibits Porcine Epidemic Diarrhea Virus Infection In Vitro Viruses PEDV molnupiravir antiviral RdRp activity RNA-Seq |
| title | Molnupiravir Inhibits Porcine Epidemic Diarrhea Virus Infection In Vitro |
| title_full | Molnupiravir Inhibits Porcine Epidemic Diarrhea Virus Infection In Vitro |
| title_fullStr | Molnupiravir Inhibits Porcine Epidemic Diarrhea Virus Infection In Vitro |
| title_full_unstemmed | Molnupiravir Inhibits Porcine Epidemic Diarrhea Virus Infection In Vitro |
| title_short | Molnupiravir Inhibits Porcine Epidemic Diarrhea Virus Infection In Vitro |
| title_sort | molnupiravir inhibits porcine epidemic diarrhea virus infection in vitro |
| topic | PEDV molnupiravir antiviral RdRp activity RNA-Seq |
| url | https://www.mdpi.com/1999-4915/15/6/1317 |
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