Investigating the role of Zibai ointment on apoptosis‐related factors Bcl‐2 and Bax in wound healing after anal fistula surgery
Abstract Objective In this study, we investigated the impact of Zibai ointment on wound healing by analyzing the expression levels of two key apoptosis‐related factors—B‐cell lymphoma 2 (Bcl‐2) and Bcl‐2‐associated X protein (Bax), in patients following surgery for anal fistula. Methods We included...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-06-01
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| Series: | Immunity, Inflammation and Disease |
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| Online Access: | https://doi.org/10.1002/iid3.912 |
| _version_ | 1827903495865368576 |
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| author | Er‐Wei Cai Cheng Zhao Wei‐Juan Wang Zhen‐Peng Xu Feng Lin |
| author_facet | Er‐Wei Cai Cheng Zhao Wei‐Juan Wang Zhen‐Peng Xu Feng Lin |
| author_sort | Er‐Wei Cai |
| collection | DOAJ |
| description | Abstract Objective In this study, we investigated the impact of Zibai ointment on wound healing by analyzing the expression levels of two key apoptosis‐related factors—B‐cell lymphoma 2 (Bcl‐2) and Bcl‐2‐associated X protein (Bax), in patients following surgery for anal fistula. Methods We included 90 patients with anal fistulas who were treated in the People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine. Patients were randomly assigned to receive treatment with Zibai ointment (n = 45) or petroleum jelly (n = 45). The levels of apoptosis‐related factors Bcl‐2 and Bax were evaluated using enzyme‐linked immunosorbent assay (ELISA), while cell apoptosis was assessed using Terminal deoxynucleotidyl transferase (TdT) dUTP Nick‐End Labeling (TUNEL) assay. Results The results of ELISA showed that on Day 21 after the surgery, the levels of Bcl‐2 and Bax in the Zibai ointment group were significantly different compared to the petroleum jelly group, with values of (60.11 ± 1.31) ng/mL and (7.05 ± 0.01) versus (83.79 ± 1.74) ng/mL and (6.00 ± 0.05) ng/mL, respectively (p < .05). Furthermore, light microscopy revealed a large number of apoptotic cells within the field of vision 14 days postsurgery in the Zibai ointment group, and the healing time in the Zibai ointment group was significantly different from that in the petroleum jelly group (p < .05). Conclusion We found that Zibai ointment effectively promoted wound healing in patients following anal fistula surgery, possibly by regulating Bcl‐2 and Bax apoptosis‐related factors. |
| first_indexed | 2024-03-13T00:13:51Z |
| format | Article |
| id | doaj.art-b6a7a10fa84a4cebb04066c870eba2c9 |
| institution | Directory Open Access Journal |
| issn | 2050-4527 |
| language | English |
| last_indexed | 2024-03-13T00:13:51Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Immunity, Inflammation and Disease |
| spelling | doaj.art-b6a7a10fa84a4cebb04066c870eba2c92023-07-12T07:20:49ZengWileyImmunity, Inflammation and Disease2050-45272023-06-01116n/an/a10.1002/iid3.912Investigating the role of Zibai ointment on apoptosis‐related factors Bcl‐2 and Bax in wound healing after anal fistula surgeryEr‐Wei Cai0Cheng Zhao1Wei‐Juan Wang2Zhen‐Peng Xu3Feng Lin4The Second Department of Anorectal The Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine Fujian ChinaThe Second Department of Anorectal The Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine Fujian ChinaParamedics The Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine Fujian ChinaThe Second Department of Anorectal The Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine Fujian ChinaThe Second Department of Anorectal The Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine Fujian ChinaAbstract Objective In this study, we investigated the impact of Zibai ointment on wound healing by analyzing the expression levels of two key apoptosis‐related factors—B‐cell lymphoma 2 (Bcl‐2) and Bcl‐2‐associated X protein (Bax), in patients following surgery for anal fistula. Methods We included 90 patients with anal fistulas who were treated in the People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine. Patients were randomly assigned to receive treatment with Zibai ointment (n = 45) or petroleum jelly (n = 45). The levels of apoptosis‐related factors Bcl‐2 and Bax were evaluated using enzyme‐linked immunosorbent assay (ELISA), while cell apoptosis was assessed using Terminal deoxynucleotidyl transferase (TdT) dUTP Nick‐End Labeling (TUNEL) assay. Results The results of ELISA showed that on Day 21 after the surgery, the levels of Bcl‐2 and Bax in the Zibai ointment group were significantly different compared to the petroleum jelly group, with values of (60.11 ± 1.31) ng/mL and (7.05 ± 0.01) versus (83.79 ± 1.74) ng/mL and (6.00 ± 0.05) ng/mL, respectively (p < .05). Furthermore, light microscopy revealed a large number of apoptotic cells within the field of vision 14 days postsurgery in the Zibai ointment group, and the healing time in the Zibai ointment group was significantly different from that in the petroleum jelly group (p < .05). Conclusion We found that Zibai ointment effectively promoted wound healing in patients following anal fistula surgery, possibly by regulating Bcl‐2 and Bax apoptosis‐related factors.https://doi.org/10.1002/iid3.912anal fistulaBaxBcl‐2Zibai ointment |
| spellingShingle | Er‐Wei Cai Cheng Zhao Wei‐Juan Wang Zhen‐Peng Xu Feng Lin Investigating the role of Zibai ointment on apoptosis‐related factors Bcl‐2 and Bax in wound healing after anal fistula surgery Immunity, Inflammation and Disease anal fistula Bax Bcl‐2 Zibai ointment |
| title | Investigating the role of Zibai ointment on apoptosis‐related factors Bcl‐2 and Bax in wound healing after anal fistula surgery |
| title_full | Investigating the role of Zibai ointment on apoptosis‐related factors Bcl‐2 and Bax in wound healing after anal fistula surgery |
| title_fullStr | Investigating the role of Zibai ointment on apoptosis‐related factors Bcl‐2 and Bax in wound healing after anal fistula surgery |
| title_full_unstemmed | Investigating the role of Zibai ointment on apoptosis‐related factors Bcl‐2 and Bax in wound healing after anal fistula surgery |
| title_short | Investigating the role of Zibai ointment on apoptosis‐related factors Bcl‐2 and Bax in wound healing after anal fistula surgery |
| title_sort | investigating the role of zibai ointment on apoptosis related factors bcl 2 and bax in wound healing after anal fistula surgery |
| topic | anal fistula Bax Bcl‐2 Zibai ointment |
| url | https://doi.org/10.1002/iid3.912 |
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