| Summary: | Tea is rich in catechins and aluminum. In this study, the process of catechin photolysis was applied as a model for examining the effects of aluminum chloride (AlCl<sub>3</sub>) on the structural changes of catechin and the alteration of aluminum complexes under blue light irradiation (BLI) at pH 8 using liquid chromatography and mass spectrometry techniques. Additionally, the effects of anions on catechin upon the addition of AlCl<sub>3</sub> and treatment with BLI were also studied. In this study, when 1 mM catechin was treated with BLI, a superoxide anion radical (<inline-formula><math display="inline"><semantics><mrow><msubsup><mi mathvariant="normal">O</mi><mn>2</mn><mrow><mo>•</mo><mo>−</mo></mrow></msubsup></mrow></semantics></math></inline-formula>) was generated in an air-saturated aqueous solution, in addition to forming a dimeric catechin (proanthocyanidin) via a photon-induced redox reaction. The relative percentage of catechin was found to be 59.0 and 95.7 for catechin treated with BLI and catechin upon the addition of 1 mM AlCl<sub>3</sub> treated with BLI, respectively. It suggested that catechin treated with BLI could be suppressed by AlCl<sub>3</sub>, while AlCl<sub>3</sub> did not form a complex with catechin in the photolytic system. However, under the same conditions, it was also found that the addition of AlCl<sub>3</sub> inhibited the photolytic formation of <inline-formula><math display="inline"><semantics><mrow><msubsup><mi mathvariant="normal">O</mi><mn>2</mn><mrow><mo>•</mo><mo>−</mo></mrow></msubsup><mo>,</mo></mrow></semantics></math></inline-formula> and reduced the generation of proanthocyanidin, suggesting that the disconnection of proanthocyanidin was achieved by AlCl<sub>3</sub> acting as a catalyst under treatment with BLI. The influence of 1 mM fluoride (<inline-formula><math display="inline"><semantics><mrow><msup><mi mathvariant="normal">F</mi><mo>−</mo></msup></mrow></semantics></math></inline-formula>) and 1 mM oxalate (<inline-formula><math display="inline"><semantics><mrow><msub><mi mathvariant="normal">C</mi><mn>2</mn></msub><msubsup><mi mathvariant="normal">O</mi><mn>4</mn><mrow><mn>2</mn><mo>−</mo></mrow></msubsup></mrow></semantics></math></inline-formula>) ions on the photolysis of 1 mM catechin upon the addition of 1 mM AlCl<sub>3</sub> and treatment with BLI was found to be insignificant, implying that, during the photolysis of catechin, the Al species were either neutral or negatively charged and the aluminum species did not form a complex with anions in the photolytic system. Therefore, aluminum, which is an amphoteric species, has an inherent potential to stabilize the photolysis of catechin in an alkaline conditions, while suppressing the <inline-formula><math display="inline"><semantics><mrow><msubsup><mi mathvariant="normal">O</mi><mn>2</mn><mrow><mo>•</mo><mo>−</mo></mrow></msubsup></mrow></semantics></math></inline-formula> and proanthocyanidin generation via aluminum ion catalysis in the catechin/Al system under treatment with BLI.
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