16S rRNA Gene Sequencing-Based Identification and Comparative Analysis of the Fecal Microbiota of Five Syntopic Lizard Species from a Low-Mountain Area in Western Bulgaria
Studies on the gut microbiome of free-living reptiles in Europe are generally fragmentary and still missing in Bulgaria. We aimed to identify and compare the fecal microbiota profiles of five syntopic lizard species from three families: the European green lizard (<i>Lacerta viridis</i>),...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Applied Microbiology |
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| Online Access: | https://www.mdpi.com/2673-8007/4/1/13 |
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| author | Irina Lazarkevich Stephan Engibarov Simona Mitova Emiliya Vacheva Steliyana Popova Nikola Stanchev Rumyana Eneva Yana Gocheva Ivanka Boyadzhieva Maria Gerginova |
| author_facet | Irina Lazarkevich Stephan Engibarov Simona Mitova Emiliya Vacheva Steliyana Popova Nikola Stanchev Rumyana Eneva Yana Gocheva Ivanka Boyadzhieva Maria Gerginova |
| author_sort | Irina Lazarkevich |
| collection | DOAJ |
| description | Studies on the gut microbiome of free-living reptiles in Europe are generally fragmentary and still missing in Bulgaria. We aimed to identify and compare the fecal microbiota profiles of five syntopic lizard species from three families: the European green lizard (<i>Lacerta viridis</i>), the common wall lizard (<i>Podarcis muralis</i>), the meadow lizard (<i>Darevskia praticola</i>) (Lacertidae), the European snake-eyed skink (<i>Ablepharus kitaibelii</i>) (Scincidae), and the European slow worm (<i>Anguis fragilis</i>) (Anguidae), which coinhabit a low mountainous area in the western part of the country. A high-throughput sequencing of the hypervariable V3-V4 region of the 16S rRNA gene, performed on the Illumina HiSeq2500 platform, was used. The core microbiota of lizard hosts seems to be species-specific. A dynamic phyla proportion between hosts was found. The richest alpha diversity was observed in <i>D. praticola</i>, and the lowest alpha diversity was observed in <i>P. muralis</i> and <i>A. fragilis.</i> Within the three lacertids, the microbiota of <i>D. praticola</i> and <i>L. viridis</i> were more closely related to each other than they were to those of <i>P. muralis</i>. Sharing a largely common trophic resource (all species except <i>A. fragilis</i> are mainly insectivorous) was not an indication of similarity in their gut microbial communities. |
| first_indexed | 2024-04-24T18:35:56Z |
| format | Article |
| id | doaj.art-b6a99b2fe79a458387633f6d482cbaf7 |
| institution | Directory Open Access Journal |
| issn | 2673-8007 |
| language | English |
| last_indexed | 2024-04-24T18:35:56Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Microbiology |
| spelling | doaj.art-b6a99b2fe79a458387633f6d482cbaf72024-03-27T13:18:56ZengMDPI AGApplied Microbiology2673-80072024-01-014118119310.3390/applmicrobiol401001316S rRNA Gene Sequencing-Based Identification and Comparative Analysis of the Fecal Microbiota of Five Syntopic Lizard Species from a Low-Mountain Area in Western BulgariaIrina Lazarkevich0Stephan Engibarov1Simona Mitova2Emiliya Vacheva3Steliyana Popova4Nikola Stanchev5Rumyana Eneva6Yana Gocheva7Ivanka Boyadzhieva8Maria Gerginova9The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Str., Bl. 26, 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Str., Bl. 26, 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Str., Bl. 26, 1113 Sofia, BulgariaInstitute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 1 Tsar Osvoboditel Blvd., 1000 Sofia, BulgariaFaculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Blvd., 1164 Sofia, BulgariaFaculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Blvd., 1164 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Str., Bl. 26, 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Str., Bl. 26, 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Str., Bl. 26, 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Str., Bl. 26, 1113 Sofia, BulgariaStudies on the gut microbiome of free-living reptiles in Europe are generally fragmentary and still missing in Bulgaria. We aimed to identify and compare the fecal microbiota profiles of five syntopic lizard species from three families: the European green lizard (<i>Lacerta viridis</i>), the common wall lizard (<i>Podarcis muralis</i>), the meadow lizard (<i>Darevskia praticola</i>) (Lacertidae), the European snake-eyed skink (<i>Ablepharus kitaibelii</i>) (Scincidae), and the European slow worm (<i>Anguis fragilis</i>) (Anguidae), which coinhabit a low mountainous area in the western part of the country. A high-throughput sequencing of the hypervariable V3-V4 region of the 16S rRNA gene, performed on the Illumina HiSeq2500 platform, was used. The core microbiota of lizard hosts seems to be species-specific. A dynamic phyla proportion between hosts was found. The richest alpha diversity was observed in <i>D. praticola</i>, and the lowest alpha diversity was observed in <i>P. muralis</i> and <i>A. fragilis.</i> Within the three lacertids, the microbiota of <i>D. praticola</i> and <i>L. viridis</i> were more closely related to each other than they were to those of <i>P. muralis</i>. Sharing a largely common trophic resource (all species except <i>A. fragilis</i> are mainly insectivorous) was not an indication of similarity in their gut microbial communities.https://www.mdpi.com/2673-8007/4/1/13metagenomic analysis16S rRNA genefecal microbiotareptileslizardsLacertidae |
| spellingShingle | Irina Lazarkevich Stephan Engibarov Simona Mitova Emiliya Vacheva Steliyana Popova Nikola Stanchev Rumyana Eneva Yana Gocheva Ivanka Boyadzhieva Maria Gerginova 16S rRNA Gene Sequencing-Based Identification and Comparative Analysis of the Fecal Microbiota of Five Syntopic Lizard Species from a Low-Mountain Area in Western Bulgaria Applied Microbiology metagenomic analysis 16S rRNA gene fecal microbiota reptiles lizards Lacertidae |
| title | 16S rRNA Gene Sequencing-Based Identification and Comparative Analysis of the Fecal Microbiota of Five Syntopic Lizard Species from a Low-Mountain Area in Western Bulgaria |
| title_full | 16S rRNA Gene Sequencing-Based Identification and Comparative Analysis of the Fecal Microbiota of Five Syntopic Lizard Species from a Low-Mountain Area in Western Bulgaria |
| title_fullStr | 16S rRNA Gene Sequencing-Based Identification and Comparative Analysis of the Fecal Microbiota of Five Syntopic Lizard Species from a Low-Mountain Area in Western Bulgaria |
| title_full_unstemmed | 16S rRNA Gene Sequencing-Based Identification and Comparative Analysis of the Fecal Microbiota of Five Syntopic Lizard Species from a Low-Mountain Area in Western Bulgaria |
| title_short | 16S rRNA Gene Sequencing-Based Identification and Comparative Analysis of the Fecal Microbiota of Five Syntopic Lizard Species from a Low-Mountain Area in Western Bulgaria |
| title_sort | 16s rrna gene sequencing based identification and comparative analysis of the fecal microbiota of five syntopic lizard species from a low mountain area in western bulgaria |
| topic | metagenomic analysis 16S rRNA gene fecal microbiota reptiles lizards Lacertidae |
| url | https://www.mdpi.com/2673-8007/4/1/13 |
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