Mitochondrial COI based molecular identification of harvester termite, Anacanthotermes ochraceus (Burmeister, 1839) in Riyadh Region, the Kingdom of Saudi Arabia
Objective: Termites are well known for being the most destructive pests of household commodities as well as agricultural crops around the globe. The termite fauna (Isoptera) has about 2650 described species worldwide. Several species are the pests of crops and cause damage to wood structures. Method...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-08-01
|
| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364723002446 |
| _version_ | 1797774835839926272 |
|---|---|
| author | Mureed Husain Khawaja G. Rasool Mostafa R. Sharaf Muhammad Tufail Koko D. Sutanto Waleed S. Al-Waneen Abdulrahman S. Aldawood |
| author_facet | Mureed Husain Khawaja G. Rasool Mostafa R. Sharaf Muhammad Tufail Koko D. Sutanto Waleed S. Al-Waneen Abdulrahman S. Aldawood |
| author_sort | Mureed Husain |
| collection | DOAJ |
| description | Objective: Termites are well known for being the most destructive pests of household commodities as well as agricultural crops around the globe. The termite fauna (Isoptera) has about 2650 described species worldwide. Several species are the pests of crops and cause damage to wood structures. Methods: In the present study, 29 specimens of termites collected from different localities of the Riyadh region were identified using mitochondrial gene cytochrome c oxidase subunit I (COI) deoxyribonucleic acid (DNA) sequence. COI gene was PCR amplified using universal primers (LCO 1490 and HCO 2198). MEGA7 software was used for phylogenetic tree construction which showed that all 29 specimens grouped together in a single clade indicated close relatedness of all specimens. Results: All the obtained sequences were submitted into Genbank database and accession numbers were obtained. Phylogenetic analysis showed that all specimens of present research grouped together into a single monophyletic clade, were confirmed to be highly closely related to one another, and proved to be members of the same species. Pairwise nucleotide sequence divergence analysis showed that there was less divergence among all specimens ranging from 0% to 7.8%. Sequence analysis revealed the confirmed precise identification of 29 samples of Anacanthotermes ochraceus with COI barcode analysis. Conclusions: Molecular data analysis has confirmed morphological identification of all 29 studied samples of A. ochraceus. However, this technology offers strong support for identification of cryptic species which are difficult to identify on the basis of morphological features. Further studies of complete mitogenome can be helpful for accurate identification of termites at species level. |
| first_indexed | 2024-03-12T22:27:00Z |
| format | Article |
| id | doaj.art-b6adc84f58174194aa6faafc3af86dbf |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-03-12T22:27:00Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-b6adc84f58174194aa6faafc3af86dbf2023-07-22T04:51:34ZengElsevierJournal of King Saud University: Science1018-36472023-08-01356102782Mitochondrial COI based molecular identification of harvester termite, Anacanthotermes ochraceus (Burmeister, 1839) in Riyadh Region, the Kingdom of Saudi ArabiaMureed Husain0Khawaja G. Rasool1Mostafa R. Sharaf2Muhammad Tufail3Koko D. Sutanto4Waleed S. Al-Waneen5Abdulrahman S. Aldawood6Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia; Corresponding author.Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaPlant Protection Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaGhazi University, Dera Ghazi Khan, Punjab, PakistanPlant Protection Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaAdvanced Agricultural & Food Technologies Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh, Saudi ArabiaPlant Protection Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaObjective: Termites are well known for being the most destructive pests of household commodities as well as agricultural crops around the globe. The termite fauna (Isoptera) has about 2650 described species worldwide. Several species are the pests of crops and cause damage to wood structures. Methods: In the present study, 29 specimens of termites collected from different localities of the Riyadh region were identified using mitochondrial gene cytochrome c oxidase subunit I (COI) deoxyribonucleic acid (DNA) sequence. COI gene was PCR amplified using universal primers (LCO 1490 and HCO 2198). MEGA7 software was used for phylogenetic tree construction which showed that all 29 specimens grouped together in a single clade indicated close relatedness of all specimens. Results: All the obtained sequences were submitted into Genbank database and accession numbers were obtained. Phylogenetic analysis showed that all specimens of present research grouped together into a single monophyletic clade, were confirmed to be highly closely related to one another, and proved to be members of the same species. Pairwise nucleotide sequence divergence analysis showed that there was less divergence among all specimens ranging from 0% to 7.8%. Sequence analysis revealed the confirmed precise identification of 29 samples of Anacanthotermes ochraceus with COI barcode analysis. Conclusions: Molecular data analysis has confirmed morphological identification of all 29 studied samples of A. ochraceus. However, this technology offers strong support for identification of cryptic species which are difficult to identify on the basis of morphological features. Further studies of complete mitogenome can be helpful for accurate identification of termites at species level.http://www.sciencedirect.com/science/article/pii/S1018364723002446IsopteraBlattodeaDNA barcodingAnacanthotermes ochraceusRiyadh |
| spellingShingle | Mureed Husain Khawaja G. Rasool Mostafa R. Sharaf Muhammad Tufail Koko D. Sutanto Waleed S. Al-Waneen Abdulrahman S. Aldawood Mitochondrial COI based molecular identification of harvester termite, Anacanthotermes ochraceus (Burmeister, 1839) in Riyadh Region, the Kingdom of Saudi Arabia Journal of King Saud University: Science Isoptera Blattodea DNA barcoding Anacanthotermes ochraceus Riyadh |
| title | Mitochondrial COI based molecular identification of harvester termite, Anacanthotermes ochraceus (Burmeister, 1839) in Riyadh Region, the Kingdom of Saudi Arabia |
| title_full | Mitochondrial COI based molecular identification of harvester termite, Anacanthotermes ochraceus (Burmeister, 1839) in Riyadh Region, the Kingdom of Saudi Arabia |
| title_fullStr | Mitochondrial COI based molecular identification of harvester termite, Anacanthotermes ochraceus (Burmeister, 1839) in Riyadh Region, the Kingdom of Saudi Arabia |
| title_full_unstemmed | Mitochondrial COI based molecular identification of harvester termite, Anacanthotermes ochraceus (Burmeister, 1839) in Riyadh Region, the Kingdom of Saudi Arabia |
| title_short | Mitochondrial COI based molecular identification of harvester termite, Anacanthotermes ochraceus (Burmeister, 1839) in Riyadh Region, the Kingdom of Saudi Arabia |
| title_sort | mitochondrial coi based molecular identification of harvester termite anacanthotermes ochraceus burmeister 1839 in riyadh region the kingdom of saudi arabia |
| topic | Isoptera Blattodea DNA barcoding Anacanthotermes ochraceus Riyadh |
| url | http://www.sciencedirect.com/science/article/pii/S1018364723002446 |
| work_keys_str_mv | AT mureedhusain mitochondrialcoibasedmolecularidentificationofharvestertermiteanacanthotermesochraceusburmeister1839inriyadhregionthekingdomofsaudiarabia AT khawajagrasool mitochondrialcoibasedmolecularidentificationofharvestertermiteanacanthotermesochraceusburmeister1839inriyadhregionthekingdomofsaudiarabia AT mostafarsharaf mitochondrialcoibasedmolecularidentificationofharvestertermiteanacanthotermesochraceusburmeister1839inriyadhregionthekingdomofsaudiarabia AT muhammadtufail mitochondrialcoibasedmolecularidentificationofharvestertermiteanacanthotermesochraceusburmeister1839inriyadhregionthekingdomofsaudiarabia AT kokodsutanto mitochondrialcoibasedmolecularidentificationofharvestertermiteanacanthotermesochraceusburmeister1839inriyadhregionthekingdomofsaudiarabia AT waleedsalwaneen mitochondrialcoibasedmolecularidentificationofharvestertermiteanacanthotermesochraceusburmeister1839inriyadhregionthekingdomofsaudiarabia AT abdulrahmansaldawood mitochondrialcoibasedmolecularidentificationofharvestertermiteanacanthotermesochraceusburmeister1839inriyadhregionthekingdomofsaudiarabia |