Unanticipated domain requirements for Drosophila Wnk kinase in vivo.
WNK (With no Lysine [K]) kinases have critical roles in the maintenance of ion homeostasis and the regulation of cell volume. Their overactivation leads to pseudohypoaldosteronism type II (Gordon syndrome) characterized by hyperkalemia and high blood pressure. More recently, WNK family members have...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-10-01
|
| Series: | PLoS Genetics |
| Online Access: | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1010975&type=printable |
| _version_ | 1797311465987768320 |
|---|---|
| author | Prathibha Yarikipati Sima Jonusaite John M Pleinis Carihann Dominicci Cotto David Sanchez-Hernandez Daryl E Morrison Suhani Goyal Jeffrey Schellinger Clothilde Pénalva Jennifer Curtiss Aylin R Rodan Andreas Jenny |
| author_facet | Prathibha Yarikipati Sima Jonusaite John M Pleinis Carihann Dominicci Cotto David Sanchez-Hernandez Daryl E Morrison Suhani Goyal Jeffrey Schellinger Clothilde Pénalva Jennifer Curtiss Aylin R Rodan Andreas Jenny |
| author_sort | Prathibha Yarikipati |
| collection | DOAJ |
| description | WNK (With no Lysine [K]) kinases have critical roles in the maintenance of ion homeostasis and the regulation of cell volume. Their overactivation leads to pseudohypoaldosteronism type II (Gordon syndrome) characterized by hyperkalemia and high blood pressure. More recently, WNK family members have been shown to be required for the development of the nervous system in mice, zebrafish, and flies, and the cardiovascular system of mice and fish. Furthermore, human WNK2 and Drosophila Wnk modulate canonical Wnt signaling. In addition to a well-conserved kinase domain, animal WNKs have a large, poorly conserved C-terminal domain whose function has been largely mysterious. In most but not all cases, WNKs bind and activate downstream kinases OSR1/SPAK, which in turn regulate the activity of various ion transporters and channels. Here, we show that Drosophila Wnk regulates Wnt signaling and cell size during the development of the wing in a manner dependent on Fray, the fly homolog of OSR1/SPAK. We show that the only canonical RF(X)V/I motif of Wnk, thought to be essential for WNK interactions with OSR1/SPAK, is required to interact with Fray in vitro. However, this motif is unexpectedly dispensable for Fray-dependent Wnk functions in vivo during fly development and fluid secretion in the Malpighian (renal) tubules. In contrast, a structure function analysis of Wnk revealed that the less-conserved C-terminus of Wnk, that recently has been shown to promote phase transitions in cell culture, is required for viability in vivo. Our data thus provide novel insights into unexpected in vivo roles of specific WNK domains. |
| first_indexed | 2024-03-08T02:00:05Z |
| format | Article |
| id | doaj.art-b6adfc2ea48b4ecd889356660b33c85a |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-03-08T02:00:05Z |
| publishDate | 2023-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-b6adfc2ea48b4ecd889356660b33c85a2024-02-14T05:32:11ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042023-10-011910e101097510.1371/journal.pgen.1010975Unanticipated domain requirements for Drosophila Wnk kinase in vivo.Prathibha YarikipatiSima JonusaiteJohn M PleinisCarihann Dominicci CottoDavid Sanchez-HernandezDaryl E MorrisonSuhani GoyalJeffrey SchellingerClothilde PénalvaJennifer CurtissAylin R RodanAndreas JennyWNK (With no Lysine [K]) kinases have critical roles in the maintenance of ion homeostasis and the regulation of cell volume. Their overactivation leads to pseudohypoaldosteronism type II (Gordon syndrome) characterized by hyperkalemia and high blood pressure. More recently, WNK family members have been shown to be required for the development of the nervous system in mice, zebrafish, and flies, and the cardiovascular system of mice and fish. Furthermore, human WNK2 and Drosophila Wnk modulate canonical Wnt signaling. In addition to a well-conserved kinase domain, animal WNKs have a large, poorly conserved C-terminal domain whose function has been largely mysterious. In most but not all cases, WNKs bind and activate downstream kinases OSR1/SPAK, which in turn regulate the activity of various ion transporters and channels. Here, we show that Drosophila Wnk regulates Wnt signaling and cell size during the development of the wing in a manner dependent on Fray, the fly homolog of OSR1/SPAK. We show that the only canonical RF(X)V/I motif of Wnk, thought to be essential for WNK interactions with OSR1/SPAK, is required to interact with Fray in vitro. However, this motif is unexpectedly dispensable for Fray-dependent Wnk functions in vivo during fly development and fluid secretion in the Malpighian (renal) tubules. In contrast, a structure function analysis of Wnk revealed that the less-conserved C-terminus of Wnk, that recently has been shown to promote phase transitions in cell culture, is required for viability in vivo. Our data thus provide novel insights into unexpected in vivo roles of specific WNK domains.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1010975&type=printable |
| spellingShingle | Prathibha Yarikipati Sima Jonusaite John M Pleinis Carihann Dominicci Cotto David Sanchez-Hernandez Daryl E Morrison Suhani Goyal Jeffrey Schellinger Clothilde Pénalva Jennifer Curtiss Aylin R Rodan Andreas Jenny Unanticipated domain requirements for Drosophila Wnk kinase in vivo. PLoS Genetics |
| title | Unanticipated domain requirements for Drosophila Wnk kinase in vivo. |
| title_full | Unanticipated domain requirements for Drosophila Wnk kinase in vivo. |
| title_fullStr | Unanticipated domain requirements for Drosophila Wnk kinase in vivo. |
| title_full_unstemmed | Unanticipated domain requirements for Drosophila Wnk kinase in vivo. |
| title_short | Unanticipated domain requirements for Drosophila Wnk kinase in vivo. |
| title_sort | unanticipated domain requirements for drosophila wnk kinase in vivo |
| url | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1010975&type=printable |
| work_keys_str_mv | AT prathibhayarikipati unanticipateddomainrequirementsfordrosophilawnkkinaseinvivo AT simajonusaite unanticipateddomainrequirementsfordrosophilawnkkinaseinvivo AT johnmpleinis unanticipateddomainrequirementsfordrosophilawnkkinaseinvivo AT carihanndominiccicotto unanticipateddomainrequirementsfordrosophilawnkkinaseinvivo AT davidsanchezhernandez unanticipateddomainrequirementsfordrosophilawnkkinaseinvivo AT darylemorrison unanticipateddomainrequirementsfordrosophilawnkkinaseinvivo AT suhanigoyal unanticipateddomainrequirementsfordrosophilawnkkinaseinvivo AT jeffreyschellinger unanticipateddomainrequirementsfordrosophilawnkkinaseinvivo AT clothildepenalva unanticipateddomainrequirementsfordrosophilawnkkinaseinvivo AT jennifercurtiss unanticipateddomainrequirementsfordrosophilawnkkinaseinvivo AT aylinrrodan unanticipateddomainrequirementsfordrosophilawnkkinaseinvivo AT andreasjenny unanticipateddomainrequirementsfordrosophilawnkkinaseinvivo |