Genome-level analysis of BpR2R3-MYB family genes transcribed in seedlings of Betula platyphylla and BpR2R3-MYB15 enhanced flavonoid production
Abstract Background Flavonoids have a wide range of biological activities in plant development, stress resistance and human health, etc. R2R3-MYBs are one of the key elements in regulation of flavonoid production, but their functional importance in Betula platyphylla remains elusive. Methods The ful...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-06-01
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| Series: | Chemical and Biological Technologies in Agriculture |
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| Online Access: | https://doi.org/10.1186/s40538-022-00301-7 |
| _version_ | 1811344195514793984 |
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| author | Huimin Liu Zhongyang Yu Guizhi Fan Baojiang Zheng |
| author_facet | Huimin Liu Zhongyang Yu Guizhi Fan Baojiang Zheng |
| author_sort | Huimin Liu |
| collection | DOAJ |
| description | Abstract Background Flavonoids have a wide range of biological activities in plant development, stress resistance and human health, etc. R2R3-MYBs are one of the key elements in regulation of flavonoid production, but their functional importance in Betula platyphylla remains elusive. Methods The full-length transcriptome data of 30-day-old seedlings of Betula platyphylla were used to identify BpR2R3-MYB family genes, and their gene structure, chromosome distribution and syntenic relationships were predicted by bioinformatics methods. Agrobacterium-mediated transient transformation was used to verify the function of BpR2R3-pMYB15 in flavonoid production. Results 44 BpR2R3-MYB family genes expressed in seedlings of Betula platyphylla were identified and found to be unevenly distributed in 11 chromosomes. Among them, 90.90% of the BpR2R3-MYBs had introns, and only four genes had no introns. Five gene pairs with segment duplication were found, and their Ka/Ks ratios were less than 1. Thirty orthologs between Betula platyphylla and Arabidopsis thaliana and 68 orthologs between Betula platyphylla and Populus trichocarpa were detected. Five BpR2R3-MYBs were clustered with R2R3-MYB genes related to flavonoid synthesis, and BpR2R3-pMYB15 had the highest correlation coefficients between the value of gene expression and flavonoid content. BpR2R3-pMYB15 was cloned, and its transient overexpression obtained using Agrobacterium-mediated transformation positively regulated flavonoid production. Conclusion This work enriches the collection of R2R3-MYBs related to flavonoid production in seedlings of Betula platyphylla. Graphical Abstract |
| first_indexed | 2024-04-13T19:43:28Z |
| format | Article |
| id | doaj.art-b6ae2d9091e24692aa71e034625cf548 |
| institution | Directory Open Access Journal |
| issn | 2196-5641 |
| language | English |
| last_indexed | 2024-04-13T19:43:28Z |
| publishDate | 2022-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Chemical and Biological Technologies in Agriculture |
| spelling | doaj.art-b6ae2d9091e24692aa71e034625cf5482022-12-22T02:32:49ZengSpringerOpenChemical and Biological Technologies in Agriculture2196-56412022-06-019111410.1186/s40538-022-00301-7Genome-level analysis of BpR2R3-MYB family genes transcribed in seedlings of Betula platyphylla and BpR2R3-MYB15 enhanced flavonoid productionHuimin Liu0Zhongyang Yu1Guizhi Fan2Baojiang Zheng3Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministey of Education, Northeast Forestry UniversityKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministey of Education, Northeast Forestry UniversityKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministey of Education, Northeast Forestry UniversityKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministey of Education, Northeast Forestry UniversityAbstract Background Flavonoids have a wide range of biological activities in plant development, stress resistance and human health, etc. R2R3-MYBs are one of the key elements in regulation of flavonoid production, but their functional importance in Betula platyphylla remains elusive. Methods The full-length transcriptome data of 30-day-old seedlings of Betula platyphylla were used to identify BpR2R3-MYB family genes, and their gene structure, chromosome distribution and syntenic relationships were predicted by bioinformatics methods. Agrobacterium-mediated transient transformation was used to verify the function of BpR2R3-pMYB15 in flavonoid production. Results 44 BpR2R3-MYB family genes expressed in seedlings of Betula platyphylla were identified and found to be unevenly distributed in 11 chromosomes. Among them, 90.90% of the BpR2R3-MYBs had introns, and only four genes had no introns. Five gene pairs with segment duplication were found, and their Ka/Ks ratios were less than 1. Thirty orthologs between Betula platyphylla and Arabidopsis thaliana and 68 orthologs between Betula platyphylla and Populus trichocarpa were detected. Five BpR2R3-MYBs were clustered with R2R3-MYB genes related to flavonoid synthesis, and BpR2R3-pMYB15 had the highest correlation coefficients between the value of gene expression and flavonoid content. BpR2R3-pMYB15 was cloned, and its transient overexpression obtained using Agrobacterium-mediated transformation positively regulated flavonoid production. Conclusion This work enriches the collection of R2R3-MYBs related to flavonoid production in seedlings of Betula platyphylla. Graphical Abstracthttps://doi.org/10.1186/s40538-022-00301-7R2R3-MYBGene familyFlavonoidSeedlings of Betula platyphyllaFull-length transcriptome |
| spellingShingle | Huimin Liu Zhongyang Yu Guizhi Fan Baojiang Zheng Genome-level analysis of BpR2R3-MYB family genes transcribed in seedlings of Betula platyphylla and BpR2R3-MYB15 enhanced flavonoid production Chemical and Biological Technologies in Agriculture R2R3-MYB Gene family Flavonoid Seedlings of Betula platyphylla Full-length transcriptome |
| title | Genome-level analysis of BpR2R3-MYB family genes transcribed in seedlings of Betula platyphylla and BpR2R3-MYB15 enhanced flavonoid production |
| title_full | Genome-level analysis of BpR2R3-MYB family genes transcribed in seedlings of Betula platyphylla and BpR2R3-MYB15 enhanced flavonoid production |
| title_fullStr | Genome-level analysis of BpR2R3-MYB family genes transcribed in seedlings of Betula platyphylla and BpR2R3-MYB15 enhanced flavonoid production |
| title_full_unstemmed | Genome-level analysis of BpR2R3-MYB family genes transcribed in seedlings of Betula platyphylla and BpR2R3-MYB15 enhanced flavonoid production |
| title_short | Genome-level analysis of BpR2R3-MYB family genes transcribed in seedlings of Betula platyphylla and BpR2R3-MYB15 enhanced flavonoid production |
| title_sort | genome level analysis of bpr2r3 myb family genes transcribed in seedlings of betula platyphylla and bpr2r3 myb15 enhanced flavonoid production |
| topic | R2R3-MYB Gene family Flavonoid Seedlings of Betula platyphylla Full-length transcriptome |
| url | https://doi.org/10.1186/s40538-022-00301-7 |
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