Effects of transport stress on the oxidative index, apoptosis and autophagy in the small intestine of caprine
Abstract Background Introducing new goat breeds or transferring adult goats from farms to slaughterhouses requires transportation, which can engender adverse effects, such as oxidative stress, pathological cell apoptosis and autophagy. Current evidence suggests that malondialdehyde (MDA) is a metabo...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-08-01
|
| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12917-023-03670-9 |
| _version_ | 1797452261891244032 |
|---|---|
| author | Ruini Peng Fan Gao Yunhai Hu Kangli Li Ben Liu Wenya Zheng Xue Yang Wei Hu Lucheng Zheng Qingcan Fan Manxin Fang |
| author_facet | Ruini Peng Fan Gao Yunhai Hu Kangli Li Ben Liu Wenya Zheng Xue Yang Wei Hu Lucheng Zheng Qingcan Fan Manxin Fang |
| author_sort | Ruini Peng |
| collection | DOAJ |
| description | Abstract Background Introducing new goat breeds or transferring adult goats from farms to slaughterhouses requires transportation, which can engender adverse effects, such as oxidative stress, pathological cell apoptosis and autophagy. Current evidence suggests that malondialdehyde (MDA) is a metabolite of lipid peroxidation during oxidative stress, while superoxide dismutase (SOD) and catalase (CAT) can alleviate injury caused by free radicals and reactive oxygen species (ROS). Meanwhile, Bcl-2, Bax, LC3B, PINK1 and Parkin are important proteins that participate in pathological cell apoptosis and autophagy. This study aimed to investigate the effects of transportation stress on oxidative stress indexes and expressions of Bcl-2, Bax, LC3B, PINK1 and Parkin in the small intestine of goats. Twelve healthy adult male goats from western Jiangxi province were randomly divided into control, 2 h transportation stress, and 6 h transportation stress groups (n = 4 per group). Results Our results showed that MDA in the small intestine significantly increased after transportation, while SOD and CAT activities decreased, with a significantly increased apoptosis rate of the small intestine cells. The jejunum and duodenum exhibited the highest apoptosis rate in the 2 h and 6 h transportation groups, respectively. The expression of apoptosis-related genes Bcl-2 and Bax and their corresponding proteins exhibited varying degrees of down-regulation or up-regulation, while Bcl-2 and Bax genes in the small intestine were upregulated in the 6 h transportation group. In addition, autophagosomes and autophagolysosomes were found in various parts of the small intestine by transmission electron microscopy, and autophagy-related genes LC3B, PINK1 and Parkin were significantly down-regulated in the 2 h group and up-regulated in the 6 h group. Conclusions Our results indicate that the contents of MDA, SOD and CAT in the small intestine, the expression of pathologic apoptosis-related genes Bcl-2 and Bax, and autophagy-related genes LC3B, PINK1 and Parkin correlated with stress duration caused by transportation. Moreover, this study provides a foothold for further studies on the mechanism of transportation stress in goats and improving animal welfare. |
| first_indexed | 2024-03-09T15:06:15Z |
| format | Article |
| id | doaj.art-b6aff9833fd748428457a6db5a25ec50 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-03-09T15:06:15Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-b6aff9833fd748428457a6db5a25ec502023-11-26T13:38:31ZengBMCBMC Veterinary Research1746-61482023-08-0119111710.1186/s12917-023-03670-9Effects of transport stress on the oxidative index, apoptosis and autophagy in the small intestine of caprineRuini Peng0Fan Gao1Yunhai Hu2Kangli Li3Ben Liu4Wenya Zheng5Xue Yang6Wei Hu7Lucheng Zheng8Qingcan Fan9Manxin Fang10College of Life Science and Resources and Environment, Yichun UniversityCollege of Life Science and Resources and Environment, Yichun UniversityCollege of Life Science and Resources and Environment, Yichun UniversityCollege of Life Science and Resources and Environment, Yichun UniversityCollege of Life Science and Resources and Environment, Yichun UniversityCollege of Life Science and Resources and Environment, Yichun UniversityCollege of Life Science and Resources and Environment, Yichun UniversityCollege of Life Science and Resources and Environment, Yichun UniversityCollege of Life Science and Resources and Environment, Yichun UniversityCollege of Life Science and Resources and Environment, Yichun UniversityCollege of Life Science and Resources and Environment, Yichun UniversityAbstract Background Introducing new goat breeds or transferring adult goats from farms to slaughterhouses requires transportation, which can engender adverse effects, such as oxidative stress, pathological cell apoptosis and autophagy. Current evidence suggests that malondialdehyde (MDA) is a metabolite of lipid peroxidation during oxidative stress, while superoxide dismutase (SOD) and catalase (CAT) can alleviate injury caused by free radicals and reactive oxygen species (ROS). Meanwhile, Bcl-2, Bax, LC3B, PINK1 and Parkin are important proteins that participate in pathological cell apoptosis and autophagy. This study aimed to investigate the effects of transportation stress on oxidative stress indexes and expressions of Bcl-2, Bax, LC3B, PINK1 and Parkin in the small intestine of goats. Twelve healthy adult male goats from western Jiangxi province were randomly divided into control, 2 h transportation stress, and 6 h transportation stress groups (n = 4 per group). Results Our results showed that MDA in the small intestine significantly increased after transportation, while SOD and CAT activities decreased, with a significantly increased apoptosis rate of the small intestine cells. The jejunum and duodenum exhibited the highest apoptosis rate in the 2 h and 6 h transportation groups, respectively. The expression of apoptosis-related genes Bcl-2 and Bax and their corresponding proteins exhibited varying degrees of down-regulation or up-regulation, while Bcl-2 and Bax genes in the small intestine were upregulated in the 6 h transportation group. In addition, autophagosomes and autophagolysosomes were found in various parts of the small intestine by transmission electron microscopy, and autophagy-related genes LC3B, PINK1 and Parkin were significantly down-regulated in the 2 h group and up-regulated in the 6 h group. Conclusions Our results indicate that the contents of MDA, SOD and CAT in the small intestine, the expression of pathologic apoptosis-related genes Bcl-2 and Bax, and autophagy-related genes LC3B, PINK1 and Parkin correlated with stress duration caused by transportation. Moreover, this study provides a foothold for further studies on the mechanism of transportation stress in goats and improving animal welfare.https://doi.org/10.1186/s12917-023-03670-9GoatOxidative stress indexBcl-2BaxPINK1Parkin |
| spellingShingle | Ruini Peng Fan Gao Yunhai Hu Kangli Li Ben Liu Wenya Zheng Xue Yang Wei Hu Lucheng Zheng Qingcan Fan Manxin Fang Effects of transport stress on the oxidative index, apoptosis and autophagy in the small intestine of caprine BMC Veterinary Research Goat Oxidative stress index Bcl-2 Bax PINK1 Parkin |
| title | Effects of transport stress on the oxidative index, apoptosis and autophagy in the small intestine of caprine |
| title_full | Effects of transport stress on the oxidative index, apoptosis and autophagy in the small intestine of caprine |
| title_fullStr | Effects of transport stress on the oxidative index, apoptosis and autophagy in the small intestine of caprine |
| title_full_unstemmed | Effects of transport stress on the oxidative index, apoptosis and autophagy in the small intestine of caprine |
| title_short | Effects of transport stress on the oxidative index, apoptosis and autophagy in the small intestine of caprine |
| title_sort | effects of transport stress on the oxidative index apoptosis and autophagy in the small intestine of caprine |
| topic | Goat Oxidative stress index Bcl-2 Bax PINK1 Parkin |
| url | https://doi.org/10.1186/s12917-023-03670-9 |
| work_keys_str_mv | AT ruinipeng effectsoftransportstressontheoxidativeindexapoptosisandautophagyinthesmallintestineofcaprine AT fangao effectsoftransportstressontheoxidativeindexapoptosisandautophagyinthesmallintestineofcaprine AT yunhaihu effectsoftransportstressontheoxidativeindexapoptosisandautophagyinthesmallintestineofcaprine AT kanglili effectsoftransportstressontheoxidativeindexapoptosisandautophagyinthesmallintestineofcaprine AT benliu effectsoftransportstressontheoxidativeindexapoptosisandautophagyinthesmallintestineofcaprine AT wenyazheng effectsoftransportstressontheoxidativeindexapoptosisandautophagyinthesmallintestineofcaprine AT xueyang effectsoftransportstressontheoxidativeindexapoptosisandautophagyinthesmallintestineofcaprine AT weihu effectsoftransportstressontheoxidativeindexapoptosisandautophagyinthesmallintestineofcaprine AT luchengzheng effectsoftransportstressontheoxidativeindexapoptosisandautophagyinthesmallintestineofcaprine AT qingcanfan effectsoftransportstressontheoxidativeindexapoptosisandautophagyinthesmallintestineofcaprine AT manxinfang effectsoftransportstressontheoxidativeindexapoptosisandautophagyinthesmallintestineofcaprine |