EFFECTIVENESS OF AUXIN INDUCED IN VITRO ROOT CULTURE IN CHICORY
An efficient protocol has been developed for the root culture of (Cichorium intybus L. cv. Focus), the leaf and hypocotyl explants from 25 days old in vitro raised seedlings were cultured on half-strength Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of...
| Main Authors: | , |
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| Format: | Article |
| Language: | Bulgarian |
| Published: |
University of Zagreb, Faculty of Agriculture
2007-11-01
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| Series: | Journal of Central European Agriculture |
| Subjects: | |
| Online Access: | http://jcea.agr.hr/articles/417_EFFECTIVENESS_OF_AUXIN_INDUCED_IN_VITRO_ROOT_CULTURE_IN_CHICORY_en.pdf |
| _version_ | 1818420493414301696 |
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| author | S Nandagopal B Ranjitha Kumari |
| author_facet | S Nandagopal B Ranjitha Kumari |
| author_sort | S Nandagopal |
| collection | DOAJ |
| description | An efficient protocol has been developed for the root culture of (Cichorium intybus L. cv. Focus), the leaf and hypocotyl explants from 25 days old in vitro raised seedlings were cultured on half-strength Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of Indole-3-acetic acid (IAA), Indole-3-butyric acid (IBA), α-Napthalenacetic acid (NAA). 0.5 mg/l NAA and 0.1 mg/l IBA induced highest percentage of rooting from matured leaf explants, under total dark condition. After three weeks well established roots were separated. Fresh root tissue, in amount of 0.5 was subcultured in half-strength MS liquid medium supplemented with 0.2 mg/l NAA and 0.5 mg/l IBA, under continuous agitation at 110 rpm and total dark condition. The biomass of root culture was increased to 5.820 g after 6 weeks of culture. The root culture was maintained up to the 8 weeks. |
| first_indexed | 2024-12-14T12:55:20Z |
| format | Article |
| id | doaj.art-b6b12ef9d193415bbede11fc4ff4719a |
| institution | Directory Open Access Journal |
| issn | 1332-9049 |
| language | Bulgarian |
| last_indexed | 2024-12-14T12:55:20Z |
| publishDate | 2007-11-01 |
| publisher | University of Zagreb, Faculty of Agriculture |
| record_format | Article |
| series | Journal of Central European Agriculture |
| spelling | doaj.art-b6b12ef9d193415bbede11fc4ff4719a2022-12-21T23:00:34ZbulUniversity of Zagreb, Faculty of AgricultureJournal of Central European Agriculture1332-90492007-11-01817380EFFECTIVENESS OF AUXIN INDUCED IN VITRO ROOT CULTURE IN CHICORYS NandagopalB Ranjitha KumariAn efficient protocol has been developed for the root culture of (Cichorium intybus L. cv. Focus), the leaf and hypocotyl explants from 25 days old in vitro raised seedlings were cultured on half-strength Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of Indole-3-acetic acid (IAA), Indole-3-butyric acid (IBA), α-Napthalenacetic acid (NAA). 0.5 mg/l NAA and 0.1 mg/l IBA induced highest percentage of rooting from matured leaf explants, under total dark condition. After three weeks well established roots were separated. Fresh root tissue, in amount of 0.5 was subcultured in half-strength MS liquid medium supplemented with 0.2 mg/l NAA and 0.5 mg/l IBA, under continuous agitation at 110 rpm and total dark condition. The biomass of root culture was increased to 5.820 g after 6 weeks of culture. The root culture was maintained up to the 8 weeks.http://jcea.agr.hr/articles/417_EFFECTIVENESS_OF_AUXIN_INDUCED_IN_VITRO_ROOT_CULTURE_IN_CHICORY_en.pdfin vitro root culturematured leafhypocotylcichorium intybus l. |
| spellingShingle | S Nandagopal B Ranjitha Kumari EFFECTIVENESS OF AUXIN INDUCED IN VITRO ROOT CULTURE IN CHICORY Journal of Central European Agriculture in vitro root culture matured leaf hypocotyl cichorium intybus l. |
| title | EFFECTIVENESS OF AUXIN INDUCED IN VITRO ROOT CULTURE IN CHICORY |
| title_full | EFFECTIVENESS OF AUXIN INDUCED IN VITRO ROOT CULTURE IN CHICORY |
| title_fullStr | EFFECTIVENESS OF AUXIN INDUCED IN VITRO ROOT CULTURE IN CHICORY |
| title_full_unstemmed | EFFECTIVENESS OF AUXIN INDUCED IN VITRO ROOT CULTURE IN CHICORY |
| title_short | EFFECTIVENESS OF AUXIN INDUCED IN VITRO ROOT CULTURE IN CHICORY |
| title_sort | effectiveness of auxin induced in vitro root culture in chicory |
| topic | in vitro root culture matured leaf hypocotyl cichorium intybus l. |
| url | http://jcea.agr.hr/articles/417_EFFECTIVENESS_OF_AUXIN_INDUCED_IN_VITRO_ROOT_CULTURE_IN_CHICORY_en.pdf |
| work_keys_str_mv | AT snandagopal effectivenessofauxininducedinvitrorootcultureinchicory AT branjithakumari effectivenessofauxininducedinvitrorootcultureinchicory |