Glycosylation of Ganoderic Acid G by <i>Bacillus</i> Glycosyltransferases
<i>Ganoderma lucidum</i> is a medicinal fungus abundant in triterpenoids, its primary bioactive components. Although numerous <i>Ganoderma</i> triterpenoids have already been identified, rare <i>Ganoderma</i> triterpenoid saponins were recently discovered. To crea...
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MDPI AG
2021-09-01
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| Series: | International Journal of Molecular Sciences |
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| author | Jiumn-Yih Wu Hsiou-Yu Ding Tzi-Yuan Wang Yun-Rong Zhang Te-Sheng Chang |
| author_facet | Jiumn-Yih Wu Hsiou-Yu Ding Tzi-Yuan Wang Yun-Rong Zhang Te-Sheng Chang |
| author_sort | Jiumn-Yih Wu |
| collection | DOAJ |
| description | <i>Ganoderma lucidum</i> is a medicinal fungus abundant in triterpenoids, its primary bioactive components. Although numerous <i>Ganoderma</i> triterpenoids have already been identified, rare <i>Ganoderma</i> triterpenoid saponins were recently discovered. To create novel <i>Ganoderma</i> saponins, ganoderic acid G (GAG) was selected for biotransformation using four <i>Bacillus</i> glycosyltransferases (GTs) including BtGT_16345 from the <i>Bacillus thuringiensis</i> GA A07 strain and three GTs (BsGT110, BsUGT398, and BsUGT489) from the <i>Bacillus subtilis</i> ATCC 6633 strain. The results showed that BsUGT489 catalyzed the glycosylation of GAG to GAG-3-<i><span style="font-variant: small-caps;">o</span></i>-<i>β</i>-glucoside, while BsGT110 catalyzed the glycosylation of GAG to GAG-26-<i><span style="font-variant: small-caps;">o</span></i>-<i>β</i>-glucoside, which showed 54-fold and 97-fold greater aqueous solubility than that of GAG, respectively. To our knowledge, these two GAG saponins are new compounds. The glycosylation specificity of the four <i>Bacillus</i> GTs highlights the possibility of novel <i>Ganoderma</i> triterpenoid saponin production in the future. |
| first_indexed | 2024-03-10T07:37:02Z |
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| language | English |
| last_indexed | 2024-03-10T07:37:02Z |
| publishDate | 2021-09-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b6b470ead13948dca41792331ae04afa2023-11-22T13:26:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-09-012218974410.3390/ijms22189744Glycosylation of Ganoderic Acid G by <i>Bacillus</i> GlycosyltransferasesJiumn-Yih Wu0Hsiou-Yu Ding1Tzi-Yuan Wang2Yun-Rong Zhang3Te-Sheng Chang4Department of Food Science, National Quemoy University, Kinmen County 892, TaiwanDepartment of Cosmetic Science, Chia Nan University of Pharmacy and Science, No. 60, Erh-Jen Rd., Sec. 1, Jen-Te District, Tainan 71710, TaiwanBiodiversity Research Center, Academia Sinica, Taipei 11529, TaiwanDepartment of Biological Sciences and Technology, National University of Tainan, Tainan 70005, TaiwanDepartment of Biological Sciences and Technology, National University of Tainan, Tainan 70005, Taiwan<i>Ganoderma lucidum</i> is a medicinal fungus abundant in triterpenoids, its primary bioactive components. Although numerous <i>Ganoderma</i> triterpenoids have already been identified, rare <i>Ganoderma</i> triterpenoid saponins were recently discovered. To create novel <i>Ganoderma</i> saponins, ganoderic acid G (GAG) was selected for biotransformation using four <i>Bacillus</i> glycosyltransferases (GTs) including BtGT_16345 from the <i>Bacillus thuringiensis</i> GA A07 strain and three GTs (BsGT110, BsUGT398, and BsUGT489) from the <i>Bacillus subtilis</i> ATCC 6633 strain. The results showed that BsUGT489 catalyzed the glycosylation of GAG to GAG-3-<i><span style="font-variant: small-caps;">o</span></i>-<i>β</i>-glucoside, while BsGT110 catalyzed the glycosylation of GAG to GAG-26-<i><span style="font-variant: small-caps;">o</span></i>-<i>β</i>-glucoside, which showed 54-fold and 97-fold greater aqueous solubility than that of GAG, respectively. To our knowledge, these two GAG saponins are new compounds. The glycosylation specificity of the four <i>Bacillus</i> GTs highlights the possibility of novel <i>Ganoderma</i> triterpenoid saponin production in the future.https://www.mdpi.com/1422-0067/22/18/9744<i>Bacillus</i><i>Ganoderma</i> <i>lucidum</i>glycosyltransferasesaponintriterpenoid |
| spellingShingle | Jiumn-Yih Wu Hsiou-Yu Ding Tzi-Yuan Wang Yun-Rong Zhang Te-Sheng Chang Glycosylation of Ganoderic Acid G by <i>Bacillus</i> Glycosyltransferases International Journal of Molecular Sciences <i>Bacillus</i> <i>Ganoderma</i> <i>lucidum</i> glycosyltransferase saponin triterpenoid |
| title | Glycosylation of Ganoderic Acid G by <i>Bacillus</i> Glycosyltransferases |
| title_full | Glycosylation of Ganoderic Acid G by <i>Bacillus</i> Glycosyltransferases |
| title_fullStr | Glycosylation of Ganoderic Acid G by <i>Bacillus</i> Glycosyltransferases |
| title_full_unstemmed | Glycosylation of Ganoderic Acid G by <i>Bacillus</i> Glycosyltransferases |
| title_short | Glycosylation of Ganoderic Acid G by <i>Bacillus</i> Glycosyltransferases |
| title_sort | glycosylation of ganoderic acid g by i bacillus i glycosyltransferases |
| topic | <i>Bacillus</i> <i>Ganoderma</i> <i>lucidum</i> glycosyltransferase saponin triterpenoid |
| url | https://www.mdpi.com/1422-0067/22/18/9744 |
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