Simultaneous Quantification of Nine Target Compounds in Traditional Korean Medicine, Bopyeo-Tang, Using High-Performance Liquid Chromatography–Photodiode Array Detector and Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry
Bopyeo-tang (BPT) is composed of six medicinal herbs (<i>Morus alba</i> L., <i>Rehmannia glutinosa</i> (Gaertn.) DC., <i>Panax ginseng</i> C.A.Mey., <i>Aster tataricus</i> L.f., <i>Astragalus propinquus</i> Schischkin, and <i>Schisand...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/5/1171 |
| _version_ | 1797264109804191744 |
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| author | Chang-Seob Seo |
| author_facet | Chang-Seob Seo |
| author_sort | Chang-Seob Seo |
| collection | DOAJ |
| description | Bopyeo-tang (BPT) is composed of six medicinal herbs (<i>Morus alba</i> L., <i>Rehmannia glutinosa</i> (Gaertn.) DC., <i>Panax ginseng</i> C.A.Mey., <i>Aster tataricus</i> L.f., <i>Astragalus propinquus</i> Schischkin, and <i>Schisandra chinensis</i> (Turcz.) Baill.) and has been used for the treatment of lung diseases. This study focused on establishing an analytical method that can simultaneously quantify nine target compounds (i.e., hydroxymethylfurfural, mulberroside A, chlorogenic acid, calycosin-7-<i>O</i>-glucoside, 3,5-dicaffeoylquinic acid, quercetin, kaempferol, schizandrin, and gomisin A) from a BPT sample using high-performance liquid chromatography with a photodiode array detector (HPLC–PDA) and ultra-performance liquid chromatography with tandem mass spectrometry (UPLC–MS/MS). The separation of compounds in both analyses was performed on a C<sub>18</sub> reversed-phase column using the gradient elution of water–acetonitrile as the mobile phase. In particular, the multiple reaction monitoring mode was applied for quick and accurate detection in UPLC–MS/MS analysis. As a result of analyzing the two methods, HPLC–PDA and UPLC–MS/MS, the coefficient of determination of the regression equation for each compound was ≥0.9952, and recovery was 85.99−106.40% (relative standard deviation (RSD) < 9.58%). Precision testing of the nine compounds was verified (RSD < 10.0%). The application of these analytical assays under optimized conditions for quantitative analysis of the BPT sample gave 0.01–4.70 mg/g. Therefore, these two assays could be used successfully to gather basic data for clinical research and the quality control of BPT. |
| first_indexed | 2024-04-25T00:23:41Z |
| format | Article |
| id | doaj.art-b6b4c20fb4664624a49c046cce124c37 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-25T00:23:41Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b6b4c20fb4664624a49c046cce124c372024-03-12T16:51:22ZengMDPI AGMolecules1420-30492024-03-01295117110.3390/molecules29051171Simultaneous Quantification of Nine Target Compounds in Traditional Korean Medicine, Bopyeo-Tang, Using High-Performance Liquid Chromatography–Photodiode Array Detector and Ultra-Performance Liquid Chromatography–Tandem Mass SpectrometryChang-Seob Seo0KM Science Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of KoreaBopyeo-tang (BPT) is composed of six medicinal herbs (<i>Morus alba</i> L., <i>Rehmannia glutinosa</i> (Gaertn.) DC., <i>Panax ginseng</i> C.A.Mey., <i>Aster tataricus</i> L.f., <i>Astragalus propinquus</i> Schischkin, and <i>Schisandra chinensis</i> (Turcz.) Baill.) and has been used for the treatment of lung diseases. This study focused on establishing an analytical method that can simultaneously quantify nine target compounds (i.e., hydroxymethylfurfural, mulberroside A, chlorogenic acid, calycosin-7-<i>O</i>-glucoside, 3,5-dicaffeoylquinic acid, quercetin, kaempferol, schizandrin, and gomisin A) from a BPT sample using high-performance liquid chromatography with a photodiode array detector (HPLC–PDA) and ultra-performance liquid chromatography with tandem mass spectrometry (UPLC–MS/MS). The separation of compounds in both analyses was performed on a C<sub>18</sub> reversed-phase column using the gradient elution of water–acetonitrile as the mobile phase. In particular, the multiple reaction monitoring mode was applied for quick and accurate detection in UPLC–MS/MS analysis. As a result of analyzing the two methods, HPLC–PDA and UPLC–MS/MS, the coefficient of determination of the regression equation for each compound was ≥0.9952, and recovery was 85.99−106.40% (relative standard deviation (RSD) < 9.58%). Precision testing of the nine compounds was verified (RSD < 10.0%). The application of these analytical assays under optimized conditions for quantitative analysis of the BPT sample gave 0.01–4.70 mg/g. Therefore, these two assays could be used successfully to gather basic data for clinical research and the quality control of BPT.https://www.mdpi.com/1420-3049/29/5/1171simultaneous quantificationtraditional Korean medicineBopyeo-tangHPLC–PDAUPLC–MS/MS |
| spellingShingle | Chang-Seob Seo Simultaneous Quantification of Nine Target Compounds in Traditional Korean Medicine, Bopyeo-Tang, Using High-Performance Liquid Chromatography–Photodiode Array Detector and Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry Molecules simultaneous quantification traditional Korean medicine Bopyeo-tang HPLC–PDA UPLC–MS/MS |
| title | Simultaneous Quantification of Nine Target Compounds in Traditional Korean Medicine, Bopyeo-Tang, Using High-Performance Liquid Chromatography–Photodiode Array Detector and Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry |
| title_full | Simultaneous Quantification of Nine Target Compounds in Traditional Korean Medicine, Bopyeo-Tang, Using High-Performance Liquid Chromatography–Photodiode Array Detector and Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry |
| title_fullStr | Simultaneous Quantification of Nine Target Compounds in Traditional Korean Medicine, Bopyeo-Tang, Using High-Performance Liquid Chromatography–Photodiode Array Detector and Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry |
| title_full_unstemmed | Simultaneous Quantification of Nine Target Compounds in Traditional Korean Medicine, Bopyeo-Tang, Using High-Performance Liquid Chromatography–Photodiode Array Detector and Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry |
| title_short | Simultaneous Quantification of Nine Target Compounds in Traditional Korean Medicine, Bopyeo-Tang, Using High-Performance Liquid Chromatography–Photodiode Array Detector and Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry |
| title_sort | simultaneous quantification of nine target compounds in traditional korean medicine bopyeo tang using high performance liquid chromatography photodiode array detector and ultra performance liquid chromatography tandem mass spectrometry |
| topic | simultaneous quantification traditional Korean medicine Bopyeo-tang HPLC–PDA UPLC–MS/MS |
| url | https://www.mdpi.com/1420-3049/29/5/1171 |
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