Comprehensive somatosensory and neurological phenotyping of NCS1 knockout mice
Abstract Neuronal calcium sensor 1 (NCS1) regulates a wide range of cellular functions throughout the mammalian nervous systems. Altered NCS1 expression is associated with neurodevelopmental and neurodegenerative diseases. Previous studies focused on affective and cognitive behaviors in NCS1 knockou...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-81650-5 |
| _version_ | 1818344796415066112 |
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| author | Lien D. Nguyen Luca G. Nolte Winston J. T. Tan Denise Giuvelis Joseph Santos-Sacchi Edward Bilsky Barbara E. Ehrlich |
| author_facet | Lien D. Nguyen Luca G. Nolte Winston J. T. Tan Denise Giuvelis Joseph Santos-Sacchi Edward Bilsky Barbara E. Ehrlich |
| author_sort | Lien D. Nguyen |
| collection | DOAJ |
| description | Abstract Neuronal calcium sensor 1 (NCS1) regulates a wide range of cellular functions throughout the mammalian nervous systems. Altered NCS1 expression is associated with neurodevelopmental and neurodegenerative diseases. Previous studies focused on affective and cognitive behaviors in NCS1 knockout (KO) mice, but little is known about the physiological and pathological states associated with the loss of NCS1 in the peripheral nervous system. We previously reported that NCS1 expression was reduced following paclitaxel-induced peripheral neuropathy. Here, we comprehensively investigated the phenotypes of NCS1-KO mice through a battery of behavioral tests examining both central and peripheral nervous systems. Generally, only mild differences were observed in thermal sensation and memory acquisition between NCS1-WT and -KO male mice, but not in female mice. No differences were observed in motor performance, affective behaviors, and hearing in both sexes. These results suggest that NCS1 plays a modulatory role in sensory perceptions and cognition, particularly in male mice. NCS1 has been proposed as a pharmacological target for various diseases. Therefore, the sex-specific effects of NCS1 loss may be of clinical interest. As we examined a constitutive KO model, future studies focusing on various conditional KO models will further elucidate the precise physiological significance of NCS1. |
| first_indexed | 2024-12-13T16:52:10Z |
| format | Article |
| id | doaj.art-b6b8f1fb46154bcdb198d9beb1bac578 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-13T16:52:10Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b6b8f1fb46154bcdb198d9beb1bac5782022-12-21T23:38:01ZengNature PortfolioScientific Reports2045-23222021-01-0111111410.1038/s41598-021-81650-5Comprehensive somatosensory and neurological phenotyping of NCS1 knockout miceLien D. Nguyen0Luca G. Nolte1Winston J. T. Tan2Denise Giuvelis3Joseph Santos-Sacchi4Edward Bilsky5Barbara E. Ehrlich6Department of Pharmacology, Yale UniversityDepartment of Pharmacology, Yale UniversityDepartment of Surgery (Otolaryngology), Yale UniversityCenter for Excellence in the Neurosciences, University of New EnglandInterdepartmental Neuroscience Program, Yale UniversityCenter for Excellence in the Neurosciences, University of New EnglandDepartment of Pharmacology, Yale UniversityAbstract Neuronal calcium sensor 1 (NCS1) regulates a wide range of cellular functions throughout the mammalian nervous systems. Altered NCS1 expression is associated with neurodevelopmental and neurodegenerative diseases. Previous studies focused on affective and cognitive behaviors in NCS1 knockout (KO) mice, but little is known about the physiological and pathological states associated with the loss of NCS1 in the peripheral nervous system. We previously reported that NCS1 expression was reduced following paclitaxel-induced peripheral neuropathy. Here, we comprehensively investigated the phenotypes of NCS1-KO mice through a battery of behavioral tests examining both central and peripheral nervous systems. Generally, only mild differences were observed in thermal sensation and memory acquisition between NCS1-WT and -KO male mice, but not in female mice. No differences were observed in motor performance, affective behaviors, and hearing in both sexes. These results suggest that NCS1 plays a modulatory role in sensory perceptions and cognition, particularly in male mice. NCS1 has been proposed as a pharmacological target for various diseases. Therefore, the sex-specific effects of NCS1 loss may be of clinical interest. As we examined a constitutive KO model, future studies focusing on various conditional KO models will further elucidate the precise physiological significance of NCS1.https://doi.org/10.1038/s41598-021-81650-5 |
| spellingShingle | Lien D. Nguyen Luca G. Nolte Winston J. T. Tan Denise Giuvelis Joseph Santos-Sacchi Edward Bilsky Barbara E. Ehrlich Comprehensive somatosensory and neurological phenotyping of NCS1 knockout mice Scientific Reports |
| title | Comprehensive somatosensory and neurological phenotyping of NCS1 knockout mice |
| title_full | Comprehensive somatosensory and neurological phenotyping of NCS1 knockout mice |
| title_fullStr | Comprehensive somatosensory and neurological phenotyping of NCS1 knockout mice |
| title_full_unstemmed | Comprehensive somatosensory and neurological phenotyping of NCS1 knockout mice |
| title_short | Comprehensive somatosensory and neurological phenotyping of NCS1 knockout mice |
| title_sort | comprehensive somatosensory and neurological phenotyping of ncs1 knockout mice |
| url | https://doi.org/10.1038/s41598-021-81650-5 |
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