Probiotic cocktails accelerate baicalin metabolism in the ileum to modulate intestinal health in broiler chickens
Background Baicalin and probiotic cocktails are promising feed additives with broad application prospects. While probiotic cocktails are known to enhance intestinal health, the potential synergistic impact of combining baicalin with probiotic cocktails on the gut health of broiler chickens remains l...
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| Format: | Article |
| Language: | English |
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BMC
2024-02-01
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| Series: | Journal of Animal Science and Biotechnology |
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| Online Access: | https://doi.org/10.1186/s40104-023-00974-6 |
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| author | Mingkun Gao Chaoyong Liao Jianyang Fu Zhonghua Ning Zengpeng Lv Yuming Guo |
| author_facet | Mingkun Gao Chaoyong Liao Jianyang Fu Zhonghua Ning Zengpeng Lv Yuming Guo |
| author_sort | Mingkun Gao |
| collection | DOAJ |
| description | Background Baicalin and probiotic cocktails are promising feed additives with broad application prospects. While probiotic cocktails are known to enhance intestinal health, the potential synergistic impact of combining baicalin with probiotic cocktails on the gut health of broiler chickens remains largely unexplored. Therefore, this study aims to investigate the influence of the combined administration of baicalin and probiotic cocktails on the composition of ileal and cecal microbiota in broiler chickens to elucidate the underlying mechanisms responsible for the health-promoting effects. Results A total of 320 1-day-old male Arbor Acres broilers were divided into 4 groups, each with 8 replicates of 10 chicks per replicate. Over a period of 42 d, the birds were fed a basal diet or the same diet supplemented with 37.5 g/t baicalin (BC), 1,000 g/t probiotic cocktails (PC), or a combination of both BC (37.5 g/t) and PC (1,000 g/t). The results demonstrated that BC + PC exhibited positive synergistic effects, enhancing intestinal morphology, immune function, and barrier function. This was evidenced by increased VH/CD ratio, sIgA levels, and upregulated expression of occludin and claudin-1 (P < 0.05). 16S rRNA analysis indicated that PC potentiated the effects of BC, particularly in the ileum, where BC + PC significantly increased the α-diversity of the ileal microbiota, altered its β-diversity, and increased the relative abundance of Flavonifractor (P < 0.05), a flavonoid-metabolizing bacterium. Furthermore, Flavonifractor positively correlated with chicken ileum crypt depth (P < 0.05). While BC + PC had a limited effect on cecal microbiota structure, the PC group had a very similar microbial composition to BC + PC, suggesting that the effect of PC at the distal end of the gut overshadowed those of BC. Conclusions We demonstrated the synergistic enhancement of gut health regulation in broiler chickens by combining baicalin and probiotic cocktails. Probiotic cocktails enhanced the effects of baicalin and accelerated its metabolism in the ileum, thereby influencing the ileal microbiota structure. This study elucidates the interaction mechanism between probiotic cocktails and plant extract additives within the host microbiota. These findings provide compelling evidence for the future development of feed additive combinations. |
| first_indexed | 2024-03-07T14:47:57Z |
| format | Article |
| id | doaj.art-b6b97af7c7e84e3984ac4c3c055c5dcb |
| institution | Directory Open Access Journal |
| issn | 2049-1891 |
| language | English |
| last_indexed | 2024-03-07T14:47:57Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
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| series | Journal of Animal Science and Biotechnology |
| spelling | doaj.art-b6b97af7c7e84e3984ac4c3c055c5dcb2024-03-05T19:52:54ZengBMCJournal of Animal Science and Biotechnology2049-18912024-02-0115111610.1186/s40104-023-00974-6Probiotic cocktails accelerate baicalin metabolism in the ileum to modulate intestinal health in broiler chickensMingkun Gao0Chaoyong Liao1Jianyang Fu2Zhonghua Ning3Zengpeng Lv4Yuming Guo5State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural UniversityState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural UniversityState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural UniversityNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural UniversityState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural UniversityState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural UniversityBackground Baicalin and probiotic cocktails are promising feed additives with broad application prospects. While probiotic cocktails are known to enhance intestinal health, the potential synergistic impact of combining baicalin with probiotic cocktails on the gut health of broiler chickens remains largely unexplored. Therefore, this study aims to investigate the influence of the combined administration of baicalin and probiotic cocktails on the composition of ileal and cecal microbiota in broiler chickens to elucidate the underlying mechanisms responsible for the health-promoting effects. Results A total of 320 1-day-old male Arbor Acres broilers were divided into 4 groups, each with 8 replicates of 10 chicks per replicate. Over a period of 42 d, the birds were fed a basal diet or the same diet supplemented with 37.5 g/t baicalin (BC), 1,000 g/t probiotic cocktails (PC), or a combination of both BC (37.5 g/t) and PC (1,000 g/t). The results demonstrated that BC + PC exhibited positive synergistic effects, enhancing intestinal morphology, immune function, and barrier function. This was evidenced by increased VH/CD ratio, sIgA levels, and upregulated expression of occludin and claudin-1 (P < 0.05). 16S rRNA analysis indicated that PC potentiated the effects of BC, particularly in the ileum, where BC + PC significantly increased the α-diversity of the ileal microbiota, altered its β-diversity, and increased the relative abundance of Flavonifractor (P < 0.05), a flavonoid-metabolizing bacterium. Furthermore, Flavonifractor positively correlated with chicken ileum crypt depth (P < 0.05). While BC + PC had a limited effect on cecal microbiota structure, the PC group had a very similar microbial composition to BC + PC, suggesting that the effect of PC at the distal end of the gut overshadowed those of BC. Conclusions We demonstrated the synergistic enhancement of gut health regulation in broiler chickens by combining baicalin and probiotic cocktails. Probiotic cocktails enhanced the effects of baicalin and accelerated its metabolism in the ileum, thereby influencing the ileal microbiota structure. This study elucidates the interaction mechanism between probiotic cocktails and plant extract additives within the host microbiota. These findings provide compelling evidence for the future development of feed additive combinations.https://doi.org/10.1186/s40104-023-00974-6BaicalinGut healthIleal microbiotaProbiotic cocktailsSynergistic effects |
| spellingShingle | Mingkun Gao Chaoyong Liao Jianyang Fu Zhonghua Ning Zengpeng Lv Yuming Guo Probiotic cocktails accelerate baicalin metabolism in the ileum to modulate intestinal health in broiler chickens Journal of Animal Science and Biotechnology Baicalin Gut health Ileal microbiota Probiotic cocktails Synergistic effects |
| title | Probiotic cocktails accelerate baicalin metabolism in the ileum to modulate intestinal health in broiler chickens |
| title_full | Probiotic cocktails accelerate baicalin metabolism in the ileum to modulate intestinal health in broiler chickens |
| title_fullStr | Probiotic cocktails accelerate baicalin metabolism in the ileum to modulate intestinal health in broiler chickens |
| title_full_unstemmed | Probiotic cocktails accelerate baicalin metabolism in the ileum to modulate intestinal health in broiler chickens |
| title_short | Probiotic cocktails accelerate baicalin metabolism in the ileum to modulate intestinal health in broiler chickens |
| title_sort | probiotic cocktails accelerate baicalin metabolism in the ileum to modulate intestinal health in broiler chickens |
| topic | Baicalin Gut health Ileal microbiota Probiotic cocktails Synergistic effects |
| url | https://doi.org/10.1186/s40104-023-00974-6 |
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