Effect of damage with carborumdum in the transitory regeneration and expression of ß-glucuronidase in different explants of <em>Phaseolus vulgaris</em> var. CIAP 7247
Agrobacterium tumefaciens-mediated genetic transformation is difficult in beans. Efficiency can be improved preparing explants for the infection. The aim of this research was to determine the effect of treatment with carborundum to cotyledon nodes and organogenic callus of Phaseolus vulgaris var. CI...
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| Format: | Article |
| Language: | English |
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Universidad Central Marta Abreu de Las Villas
2010-01-01
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| Series: | Biotecnología Vegetal |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/269 |
| _version_ | 1829489053535305728 |
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| author | Idalmis Bermúdez-Caraballoso Raúl Collado Lourdes R. García Novisel Veitía Damaris Torres Carlos Romero Gert Angenon |
| author_facet | Idalmis Bermúdez-Caraballoso Raúl Collado Lourdes R. García Novisel Veitía Damaris Torres Carlos Romero Gert Angenon |
| author_sort | Idalmis Bermúdez-Caraballoso |
| collection | DOAJ |
| description | Agrobacterium tumefaciens-mediated genetic transformation is difficult in beans. Efficiency can be improved preparing explants for the infection. The aim of this research was to determine the effect of treatment with carborundum to cotyledon nodes and organogenic callus of Phaseolus vulgaris var. CIAP 7247 in the regeneration and transient expression of â-glucuronidase. A stem segment of 5-6 mm length, containing the cotyledon node, was taken three days after mature seed germination. Fragments of 4-5 mm diameter on the seventh day after the third subculture were used in the case of organogenic callus. Five treatments at different times for the application of carborundum were studied (0, 0.5, 1.0, 1.5 and 2.0 minutes). Results showed that the application of a carborundum solution (0.5g/30ml of sterile water) in agitation, caused damage on both types of explants used. These results concluded that the application of carborundum for 1.5 minutes achieved at least regeneration of multiple buds and an average 9.68 of blue spots. However, there was an increase GUS positive spot in organogenic callus with a treatment for 1.0 minute, without affecting subsequent plant regeneration of transformed tissues in this variety of beans.
Keywords: beans, calli, genetic transformation |
| first_indexed | 2024-12-15T00:01:14Z |
| format | Article |
| id | doaj.art-b6bb033fc43b44f68339d2766833ab8e |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-15T00:01:14Z |
| publishDate | 2010-01-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-b6bb033fc43b44f68339d2766833ab8e2022-12-21T22:42:53ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472010-01-01101243Effect of damage with carborumdum in the transitory regeneration and expression of ß-glucuronidase in different explants of <em>Phaseolus vulgaris</em> var. CIAP 7247Idalmis Bermúdez-CaraballosoRaúl ColladoLourdes R. GarcíaNovisel VeitíaDamaris TorresCarlos RomeroGert AngenonAgrobacterium tumefaciens-mediated genetic transformation is difficult in beans. Efficiency can be improved preparing explants for the infection. The aim of this research was to determine the effect of treatment with carborundum to cotyledon nodes and organogenic callus of Phaseolus vulgaris var. CIAP 7247 in the regeneration and transient expression of â-glucuronidase. A stem segment of 5-6 mm length, containing the cotyledon node, was taken three days after mature seed germination. Fragments of 4-5 mm diameter on the seventh day after the third subculture were used in the case of organogenic callus. Five treatments at different times for the application of carborundum were studied (0, 0.5, 1.0, 1.5 and 2.0 minutes). Results showed that the application of a carborundum solution (0.5g/30ml of sterile water) in agitation, caused damage on both types of explants used. These results concluded that the application of carborundum for 1.5 minutes achieved at least regeneration of multiple buds and an average 9.68 of blue spots. However, there was an increase GUS positive spot in organogenic callus with a treatment for 1.0 minute, without affecting subsequent plant regeneration of transformed tissues in this variety of beans. Keywords: beans, calli, genetic transformationhttps://revista.ibp.co.cu/index.php/BV/article/view/269 |
| spellingShingle | Idalmis Bermúdez-Caraballoso Raúl Collado Lourdes R. García Novisel Veitía Damaris Torres Carlos Romero Gert Angenon Effect of damage with carborumdum in the transitory regeneration and expression of ß-glucuronidase in different explants of <em>Phaseolus vulgaris</em> var. CIAP 7247 Biotecnología Vegetal |
| title | Effect of damage with carborumdum in the transitory regeneration and expression of ß-glucuronidase in different explants of <em>Phaseolus vulgaris</em> var. CIAP 7247 |
| title_full | Effect of damage with carborumdum in the transitory regeneration and expression of ß-glucuronidase in different explants of <em>Phaseolus vulgaris</em> var. CIAP 7247 |
| title_fullStr | Effect of damage with carborumdum in the transitory regeneration and expression of ß-glucuronidase in different explants of <em>Phaseolus vulgaris</em> var. CIAP 7247 |
| title_full_unstemmed | Effect of damage with carborumdum in the transitory regeneration and expression of ß-glucuronidase in different explants of <em>Phaseolus vulgaris</em> var. CIAP 7247 |
| title_short | Effect of damage with carborumdum in the transitory regeneration and expression of ß-glucuronidase in different explants of <em>Phaseolus vulgaris</em> var. CIAP 7247 |
| title_sort | effect of damage with carborumdum in the transitory regeneration and expression of ss glucuronidase in different explants of em phaseolus vulgaris em var ciap 7247 |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/269 |
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