Molecular epidemiological investigation of piroplasms carried by pet cats and dogs in an animal hospital in Guiyang, China
Piroplasmosis is a zoonotic disease mainly caused by the Babesia and Theileria parasites. Piroplasmosis is often a subclinical infection in dogs and cats that is difficult to detect and is often suspected when clinical signs such as anemia are present. It has been reported to be prevalent in China....
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1266583/full |
| _version_ | 1797660939834621952 |
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| author | Shengchun Wu Jiao Meng Fuxun Yu Caomin Zhou Bin Yang Xingxing Chen Guanghong Yang Yi Sun Wuchun Cao Jiafu Jiang Jiahong Wu Lin Zhan |
| author_facet | Shengchun Wu Jiao Meng Fuxun Yu Caomin Zhou Bin Yang Xingxing Chen Guanghong Yang Yi Sun Wuchun Cao Jiafu Jiang Jiahong Wu Lin Zhan |
| author_sort | Shengchun Wu |
| collection | DOAJ |
| description | Piroplasmosis is a zoonotic disease mainly caused by the Babesia and Theileria parasites. Piroplasmosis is often a subclinical infection in dogs and cats that is difficult to detect and is often suspected when clinical signs such as anemia are present. It has been reported to be prevalent in China. However, molecular evidence of the disease has not been reported in pet dogs and cats in Guiyang. In this study, we collected 307 anticoagulated blood samples from an animal hospital in the Wudang District of Guiyang during the period March 2021 to November 2021 and extracted DNA from the samples. The 18S rDNA gene was amplified using PCR, and the positive amplification product was sequenced. The sequences were then analyzed for homology and phylogeny. Of the 307 samples collected, 164 were feline and 143 were canine, with a total of 23 amplifying a target band of approximately 400 bp. The percentage of positives of piroplasms infection in pet cats was 4.27% (7/164), with the pathogens being T. uilenbergi (3) and T. luwenshuni (4). One Colpodella sp. and two undetermined species were also detected in the cat samples. The percentage of positives of piroplasms infection in pet dogs was 7.69% (11/143), with the pathogen being T. uilenbergi (11). One Colpodella sp. was also detected in the dog samples. The results confirmed that T. uilenbergi and T. luwenshuni are prevalent in pet cats and dogs in this area. In addition, the study found a rare zoonotic pathogen, Colpodella sp., in cats and dogs. Therefore, this study is expected to serve as a valuable reference for decision-making regarding animal health management and public health work. |
| first_indexed | 2024-03-11T18:38:08Z |
| format | Article |
| id | doaj.art-b6bc95ce04114ca8b4a4c25e3f164480 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-11T18:38:08Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-b6bc95ce04114ca8b4a4c25e3f1644802023-10-12T16:43:17ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-10-011410.3389/fmicb.2023.12665831266583Molecular epidemiological investigation of piroplasms carried by pet cats and dogs in an animal hospital in Guiyang, ChinaShengchun Wu0Jiao Meng1Fuxun Yu2Caomin Zhou3Bin Yang4Xingxing Chen5Guanghong Yang6Yi Sun7Wuchun Cao8Jiafu Jiang9Jiahong Wu10Lin Zhan11School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, ChinaSchool of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, ChinaNHC Key Laboratory of Pulmonary Immune-related Diseases, Guizhou Provincial People’s Hospital, Guiyang, ChinaRenal Division, Department of Medicine, Guizhou Provincial People’s Hospital, Guizhou Provincial Institute of Nephritic and Urinary Disease, Guiyang, ChinaNHC Key Laboratory of Pulmonary Immune-related Diseases, Guizhou Provincial People’s Hospital, Guiyang, ChinaNHC Key Laboratory of Pulmonary Immune-related Diseases, Guizhou Provincial People’s Hospital, Guiyang, ChinaGuizhou Center for Disease Control and Prevention, Guiyang, ChinaBeijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, ChinaBeijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, ChinaBeijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, ChinaSchool of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, ChinaNHC Key Laboratory of Pulmonary Immune-related Diseases, Guizhou Provincial People’s Hospital, Guiyang, ChinaPiroplasmosis is a zoonotic disease mainly caused by the Babesia and Theileria parasites. Piroplasmosis is often a subclinical infection in dogs and cats that is difficult to detect and is often suspected when clinical signs such as anemia are present. It has been reported to be prevalent in China. However, molecular evidence of the disease has not been reported in pet dogs and cats in Guiyang. In this study, we collected 307 anticoagulated blood samples from an animal hospital in the Wudang District of Guiyang during the period March 2021 to November 2021 and extracted DNA from the samples. The 18S rDNA gene was amplified using PCR, and the positive amplification product was sequenced. The sequences were then analyzed for homology and phylogeny. Of the 307 samples collected, 164 were feline and 143 were canine, with a total of 23 amplifying a target band of approximately 400 bp. The percentage of positives of piroplasms infection in pet cats was 4.27% (7/164), with the pathogens being T. uilenbergi (3) and T. luwenshuni (4). One Colpodella sp. and two undetermined species were also detected in the cat samples. The percentage of positives of piroplasms infection in pet dogs was 7.69% (11/143), with the pathogen being T. uilenbergi (11). One Colpodella sp. was also detected in the dog samples. The results confirmed that T. uilenbergi and T. luwenshuni are prevalent in pet cats and dogs in this area. In addition, the study found a rare zoonotic pathogen, Colpodella sp., in cats and dogs. Therefore, this study is expected to serve as a valuable reference for decision-making regarding animal health management and public health work.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1266583/fulldogcatphylogenetic studiespiroplasmsTheileria uilenbergiTheileria luwenshuni |
| spellingShingle | Shengchun Wu Jiao Meng Fuxun Yu Caomin Zhou Bin Yang Xingxing Chen Guanghong Yang Yi Sun Wuchun Cao Jiafu Jiang Jiahong Wu Lin Zhan Molecular epidemiological investigation of piroplasms carried by pet cats and dogs in an animal hospital in Guiyang, China Frontiers in Microbiology dog cat phylogenetic studies piroplasms Theileria uilenbergi Theileria luwenshuni |
| title | Molecular epidemiological investigation of piroplasms carried by pet cats and dogs in an animal hospital in Guiyang, China |
| title_full | Molecular epidemiological investigation of piroplasms carried by pet cats and dogs in an animal hospital in Guiyang, China |
| title_fullStr | Molecular epidemiological investigation of piroplasms carried by pet cats and dogs in an animal hospital in Guiyang, China |
| title_full_unstemmed | Molecular epidemiological investigation of piroplasms carried by pet cats and dogs in an animal hospital in Guiyang, China |
| title_short | Molecular epidemiological investigation of piroplasms carried by pet cats and dogs in an animal hospital in Guiyang, China |
| title_sort | molecular epidemiological investigation of piroplasms carried by pet cats and dogs in an animal hospital in guiyang china |
| topic | dog cat phylogenetic studies piroplasms Theileria uilenbergi Theileria luwenshuni |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1266583/full |
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