Bacteriostatic Behavior of PLA-BaTiO<sub>3</sub> Composite Fibers Synthesized by Centrifugal Spinning and Subjected to Aging Test
The present work investigated the effect of Polylactic acid (PLA) fibers produced by centrifugal spinning with incorporated BaTiO<sub>3</sub> particles to improve their bacteriostatic behavior. The PLA matrix and three composites, presenting three different amounts of fillers, were subje...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/10/2918 |
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| author | Francesco Boschetto Hoan Ngoc Doan Phu Phong Vo Matteo Zanocco Kenta Yamamoto Wenliang Zhu Tetsuya Adachi Kenji Kinashi Elia Marin Giuseppe Pezzotti |
| author_facet | Francesco Boschetto Hoan Ngoc Doan Phu Phong Vo Matteo Zanocco Kenta Yamamoto Wenliang Zhu Tetsuya Adachi Kenji Kinashi Elia Marin Giuseppe Pezzotti |
| author_sort | Francesco Boschetto |
| collection | DOAJ |
| description | The present work investigated the effect of Polylactic acid (PLA) fibers produced by centrifugal spinning with incorporated BaTiO<sub>3</sub> particles to improve their bacteriostatic behavior. The PLA matrix and three composites, presenting three different amounts of fillers, were subjected to UV/O<sub>3</sub> treatment monitoring the possible modifications that occurred over time. The morphological and physical properties of the surfaces were characterized by different microscopic techniques, contact angle, and surface potential measurements. Subsequently, the samples were tested in vitro with human dermal fibroblasts (HDF) to verify the cytotoxicity of the substrates. No significant differences between the PLA matrix and composites emerged; the high hydrophobicity of the fibers, derived by the polymer structure, represented an obstacle limiting the fibroblast attachment. Samples underwent bacterial exposure (<i>Staphylococcus epidermidis</i>) for 12 and 24 h. Increasing the concentration of BT, the number of living bacteria and their distribution decreased in comparison with the PLA matrix suggesting an effect of the inorganic filler, which generates a neutralization effect leading to reactive oxygen species (ROS) generation and subsequently to bacterial damages. These results suggest that the barium titanate (BT) fillers clearly improve the antibacterial properties of PLA fibers after aging tests made before bacterial exposure, representing a potential candidate in the creation of composites for medical applications. |
| first_indexed | 2024-03-10T10:50:05Z |
| format | Article |
| id | doaj.art-b6bf336bfc6141f7b45225227f3626d0 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T10:50:05Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b6bf336bfc6141f7b45225227f3626d02023-11-21T22:21:15ZengMDPI AGMolecules1420-30492021-05-012610291810.3390/molecules26102918Bacteriostatic Behavior of PLA-BaTiO<sub>3</sub> Composite Fibers Synthesized by Centrifugal Spinning and Subjected to Aging TestFrancesco Boschetto0Hoan Ngoc Doan1Phu Phong Vo2Matteo Zanocco3Kenta Yamamoto4Wenliang Zhu5Tetsuya Adachi6Kenji Kinashi7Elia Marin8Giuseppe Pezzotti9Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, JapanFunctional Polymer Design Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, JapanFunctional Polymer Design Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, JapanCeramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, JapanDepartment of Dental Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, JapanCeramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, JapanDepartment of Dental Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, JapanFunctional Polymer Design Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, JapanCeramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, JapanCeramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, JapanThe present work investigated the effect of Polylactic acid (PLA) fibers produced by centrifugal spinning with incorporated BaTiO<sub>3</sub> particles to improve their bacteriostatic behavior. The PLA matrix and three composites, presenting three different amounts of fillers, were subjected to UV/O<sub>3</sub> treatment monitoring the possible modifications that occurred over time. The morphological and physical properties of the surfaces were characterized by different microscopic techniques, contact angle, and surface potential measurements. Subsequently, the samples were tested in vitro with human dermal fibroblasts (HDF) to verify the cytotoxicity of the substrates. No significant differences between the PLA matrix and composites emerged; the high hydrophobicity of the fibers, derived by the polymer structure, represented an obstacle limiting the fibroblast attachment. Samples underwent bacterial exposure (<i>Staphylococcus epidermidis</i>) for 12 and 24 h. Increasing the concentration of BT, the number of living bacteria and their distribution decreased in comparison with the PLA matrix suggesting an effect of the inorganic filler, which generates a neutralization effect leading to reactive oxygen species (ROS) generation and subsequently to bacterial damages. These results suggest that the barium titanate (BT) fillers clearly improve the antibacterial properties of PLA fibers after aging tests made before bacterial exposure, representing a potential candidate in the creation of composites for medical applications.https://www.mdpi.com/1420-3049/26/10/2918bacteriostatic behaviorpolylactic acidcomposite fibersbarium titanateneutralization |
| spellingShingle | Francesco Boschetto Hoan Ngoc Doan Phu Phong Vo Matteo Zanocco Kenta Yamamoto Wenliang Zhu Tetsuya Adachi Kenji Kinashi Elia Marin Giuseppe Pezzotti Bacteriostatic Behavior of PLA-BaTiO<sub>3</sub> Composite Fibers Synthesized by Centrifugal Spinning and Subjected to Aging Test Molecules bacteriostatic behavior polylactic acid composite fibers barium titanate neutralization |
| title | Bacteriostatic Behavior of PLA-BaTiO<sub>3</sub> Composite Fibers Synthesized by Centrifugal Spinning and Subjected to Aging Test |
| title_full | Bacteriostatic Behavior of PLA-BaTiO<sub>3</sub> Composite Fibers Synthesized by Centrifugal Spinning and Subjected to Aging Test |
| title_fullStr | Bacteriostatic Behavior of PLA-BaTiO<sub>3</sub> Composite Fibers Synthesized by Centrifugal Spinning and Subjected to Aging Test |
| title_full_unstemmed | Bacteriostatic Behavior of PLA-BaTiO<sub>3</sub> Composite Fibers Synthesized by Centrifugal Spinning and Subjected to Aging Test |
| title_short | Bacteriostatic Behavior of PLA-BaTiO<sub>3</sub> Composite Fibers Synthesized by Centrifugal Spinning and Subjected to Aging Test |
| title_sort | bacteriostatic behavior of pla batio sub 3 sub composite fibers synthesized by centrifugal spinning and subjected to aging test |
| topic | bacteriostatic behavior polylactic acid composite fibers barium titanate neutralization |
| url | https://www.mdpi.com/1420-3049/26/10/2918 |
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