Phospholipase C is a novel regulator at the early stages of microspore embryogenesis in Nicotiana tabacum
Microspore transfers the developmental fate into embryogenesis in vitro regulated by determinant factors of stress-induced. However, the key regulators of microspore embryogenesis (ME) are still largely undiscovered to reveal the mechanism of cell fate transition. Here, we report that Phospholipase...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2022-12-01
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| Series: | Plant Signaling & Behavior |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592324.2022.2094618 |
| _version_ | 1797351983378595840 |
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| author | Pan Luo Aixi Jiang Yi Zhou Mingchun Yang Xiaotong Zhou Yong Yang Jun Yu Xingchun Tang |
| author_facet | Pan Luo Aixi Jiang Yi Zhou Mingchun Yang Xiaotong Zhou Yong Yang Jun Yu Xingchun Tang |
| author_sort | Pan Luo |
| collection | DOAJ |
| description | Microspore transfers the developmental fate into embryogenesis in vitro regulated by determinant factors of stress-induced. However, the key regulators of microspore embryogenesis (ME) are still largely undiscovered to reveal the mechanism of cell fate transition. Here, we report that Phospholipase C (PLC) is involved at the early stages of ME in Nicotiana tabacum. NtPLC2/3/4 are expressed at the initial stages of ME. The expression levels of NtPLC2/3 are transient activated after 3 days in culture, while the expression level of NtPLC4 maintains relatively stable. Inhibition of PLCs induces the decrease in NtPLC2/3/4 expression level and decline of ME yield. We confirm that lipids in microspore are degraded and then re-accumulate at first embryonic division stage. Inhibition of PLCs suppresses the lipids metabolism at the early stages of ME. Thus, we propose that PLCs-mediated lipid metabolism is a novel regulator at the early stages of ME. |
| first_indexed | 2024-03-08T13:08:38Z |
| format | Article |
| id | doaj.art-b6c1e611866f420491c20f9805869b36 |
| institution | Directory Open Access Journal |
| issn | 1559-2316 1559-2324 |
| language | English |
| last_indexed | 2024-03-08T13:08:38Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Signaling & Behavior |
| spelling | doaj.art-b6c1e611866f420491c20f9805869b362024-01-18T15:58:21ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23161559-23242022-12-0117110.1080/15592324.2022.20946182094618Phospholipase C is a novel regulator at the early stages of microspore embryogenesis in Nicotiana tabacumPan Luo0Aixi Jiang1Yi Zhou2Mingchun Yang3Xiaotong Zhou4Yong Yang5Jun Yu6Xingchun Tang7State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityTobacco Research Institute of Hubei ProvinceState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityMicrospore transfers the developmental fate into embryogenesis in vitro regulated by determinant factors of stress-induced. However, the key regulators of microspore embryogenesis (ME) are still largely undiscovered to reveal the mechanism of cell fate transition. Here, we report that Phospholipase C (PLC) is involved at the early stages of ME in Nicotiana tabacum. NtPLC2/3/4 are expressed at the initial stages of ME. The expression levels of NtPLC2/3 are transient activated after 3 days in culture, while the expression level of NtPLC4 maintains relatively stable. Inhibition of PLCs induces the decrease in NtPLC2/3/4 expression level and decline of ME yield. We confirm that lipids in microspore are degraded and then re-accumulate at first embryonic division stage. Inhibition of PLCs suppresses the lipids metabolism at the early stages of ME. Thus, we propose that PLCs-mediated lipid metabolism is a novel regulator at the early stages of ME.http://dx.doi.org/10.1080/15592324.2022.2094618phospholipase cmicrospore embryogenesisnicotiana tabacum |
| spellingShingle | Pan Luo Aixi Jiang Yi Zhou Mingchun Yang Xiaotong Zhou Yong Yang Jun Yu Xingchun Tang Phospholipase C is a novel regulator at the early stages of microspore embryogenesis in Nicotiana tabacum Plant Signaling & Behavior phospholipase c microspore embryogenesis nicotiana tabacum |
| title | Phospholipase C is a novel regulator at the early stages of microspore embryogenesis in Nicotiana tabacum |
| title_full | Phospholipase C is a novel regulator at the early stages of microspore embryogenesis in Nicotiana tabacum |
| title_fullStr | Phospholipase C is a novel regulator at the early stages of microspore embryogenesis in Nicotiana tabacum |
| title_full_unstemmed | Phospholipase C is a novel regulator at the early stages of microspore embryogenesis in Nicotiana tabacum |
| title_short | Phospholipase C is a novel regulator at the early stages of microspore embryogenesis in Nicotiana tabacum |
| title_sort | phospholipase c is a novel regulator at the early stages of microspore embryogenesis in nicotiana tabacum |
| topic | phospholipase c microspore embryogenesis nicotiana tabacum |
| url | http://dx.doi.org/10.1080/15592324.2022.2094618 |
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