The Role of Autophagy-related Proteins of Beclin-1/BECN1, LC3II, and p62/SQSTM1 in Melanoma Tumors
Purpose: The prognosis of melanoma depends on early diagnosis and timely treatment. Autophagy plays a dual role in tumor progression. In the early stages, it prevents tumor formation, while in the advanced stages it promotes tumorigenicity. This study aimed at investigating the role of autophagy in...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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West Asia Organization for Cancer Prevention
2022-01-01
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| Series: | Asian Pacific Journal of Cancer Biology |
| Subjects: | |
| Online Access: | http://www.waocp.com/journal/index.php/apjcb/article/view/781 |
| _version_ | 1797338771315752960 |
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| author | Reyhaneh Hizomi Arani Hadiseh Mohammadpour Mohammad Amin Moosavi Ahad Muhammadnejad Alireza Abdollahi Marveh Rahmati |
| author_facet | Reyhaneh Hizomi Arani Hadiseh Mohammadpour Mohammad Amin Moosavi Ahad Muhammadnejad Alireza Abdollahi Marveh Rahmati |
| author_sort | Reyhaneh Hizomi Arani |
| collection | DOAJ |
| description | Purpose: The prognosis of melanoma depends on early diagnosis and timely treatment. Autophagy plays a dual role in tumor progression. In the early stages, it prevents tumor formation, while in the advanced stages it promotes tumorigenicity. This study aimed at investigating the role of autophagy in different stages of melanoma and evaluating the relationship between autophagy and clinicopathological factors.
Methods: ATG5 and BECN1 genes expression in melanoma tumors were evaluated in a retrospective study of 5 years in the cancer institute of Tehran, Iran. The autophagy-related proteins and the correlation with clinicopathological data were also investigated in a tissue microarray series of 52 melanoma tumors following by immunohistochemical staining for the autophagy-associated proteins p62/SQSTM1 (p62), LC3II and Beclin-1/BECN1. The possibility of autophagy biomarkers was also predicted by ROC curve analysis.
Results: ATG5 and BECN1 gene expression decreased in melanoma tumors in comparison with tumor margins. However, BECN1 expression at the protein level increased with tumor progression. The expression of LC3II also raised while the p62 level declined as the tumor progressed, suggesting an increased autophagy activity during tumor development. Furthermore, melanoma ulceration was positively correlated with BECN1, LC3II and p62 expression with p<0.05, though the melanoma mitotic rate and thickness did not significantly correlate with autophagy–related proteins expression.
Conclusions: Autophagy-related proteins are suggested as potential prognostic factors in melanoma and could be considered as a therapeutic target. |
| first_indexed | 2024-03-08T09:36:06Z |
| format | Article |
| id | doaj.art-b6c2e888779f4271978a10fc7e531a84 |
| institution | Directory Open Access Journal |
| issn | 2538-4635 |
| language | English |
| last_indexed | 2024-03-08T09:36:06Z |
| publishDate | 2022-01-01 |
| publisher | West Asia Organization for Cancer Prevention |
| record_format | Article |
| series | Asian Pacific Journal of Cancer Biology |
| spelling | doaj.art-b6c2e888779f4271978a10fc7e531a842024-01-30T09:11:47ZengWest Asia Organization for Cancer PreventionAsian Pacific Journal of Cancer Biology2538-46352022-01-016426327210.31557/apjcb.2021.6.4.263-272781The Role of Autophagy-related Proteins of Beclin-1/BECN1, LC3II, and p62/SQSTM1 in Melanoma TumorsReyhaneh Hizomi Arani0Hadiseh Mohammadpour1Mohammad Amin Moosavi2Ahad Muhammadnejad3Alireza Abdollahi4Marveh Rahmati5Cancer Biology Research Center, Cancer institute of Iran, Tehran University of Medical Sciences, Tehran, Iran.Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran.Department of Molecular Medicine, National Institute of Genetic Engineering and Biotechnology, Tehran P.O. Box 14965/161, Iran.Cancer Biology Research Center, Cancer institute of Iran, Tehran University of Medical Sciences, Tehran, IranDepartment of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Cancer Biology Research Center, Cancer institute of Iran, Tehran University of Medical Sciences, Tehran, IranPurpose: The prognosis of melanoma depends on early diagnosis and timely treatment. Autophagy plays a dual role in tumor progression. In the early stages, it prevents tumor formation, while in the advanced stages it promotes tumorigenicity. This study aimed at investigating the role of autophagy in different stages of melanoma and evaluating the relationship between autophagy and clinicopathological factors. Methods: ATG5 and BECN1 genes expression in melanoma tumors were evaluated in a retrospective study of 5 years in the cancer institute of Tehran, Iran. The autophagy-related proteins and the correlation with clinicopathological data were also investigated in a tissue microarray series of 52 melanoma tumors following by immunohistochemical staining for the autophagy-associated proteins p62/SQSTM1 (p62), LC3II and Beclin-1/BECN1. The possibility of autophagy biomarkers was also predicted by ROC curve analysis. Results: ATG5 and BECN1 gene expression decreased in melanoma tumors in comparison with tumor margins. However, BECN1 expression at the protein level increased with tumor progression. The expression of LC3II also raised while the p62 level declined as the tumor progressed, suggesting an increased autophagy activity during tumor development. Furthermore, melanoma ulceration was positively correlated with BECN1, LC3II and p62 expression with p<0.05, though the melanoma mitotic rate and thickness did not significantly correlate with autophagy–related proteins expression. Conclusions: Autophagy-related proteins are suggested as potential prognostic factors in melanoma and could be considered as a therapeutic target.http://www.waocp.com/journal/index.php/apjcb/article/view/781autophagy, becn1, lc3ii, melanoma, p62 |
| spellingShingle | Reyhaneh Hizomi Arani Hadiseh Mohammadpour Mohammad Amin Moosavi Ahad Muhammadnejad Alireza Abdollahi Marveh Rahmati The Role of Autophagy-related Proteins of Beclin-1/BECN1, LC3II, and p62/SQSTM1 in Melanoma Tumors Asian Pacific Journal of Cancer Biology autophagy, becn1, lc3ii, melanoma, p62 |
| title | The Role of Autophagy-related Proteins of Beclin-1/BECN1, LC3II, and p62/SQSTM1 in Melanoma Tumors |
| title_full | The Role of Autophagy-related Proteins of Beclin-1/BECN1, LC3II, and p62/SQSTM1 in Melanoma Tumors |
| title_fullStr | The Role of Autophagy-related Proteins of Beclin-1/BECN1, LC3II, and p62/SQSTM1 in Melanoma Tumors |
| title_full_unstemmed | The Role of Autophagy-related Proteins of Beclin-1/BECN1, LC3II, and p62/SQSTM1 in Melanoma Tumors |
| title_short | The Role of Autophagy-related Proteins of Beclin-1/BECN1, LC3II, and p62/SQSTM1 in Melanoma Tumors |
| title_sort | role of autophagy related proteins of beclin 1 becn1 lc3ii and p62 sqstm1 in melanoma tumors |
| topic | autophagy, becn1, lc3ii, melanoma, p62 |
| url | http://www.waocp.com/journal/index.php/apjcb/article/view/781 |
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