Regeneration and reprogramming compared
<p>Abstract</p> <p>Background</p> <p>Dedifferentiation occurs naturally in mature cell types during epimorphic regeneration in fish and some amphibians. Dedifferentiation also occurs in the induction of pluripotent stem cells when a set of transcription factors (<it&...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-01-01
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| Series: | BMC Biology |
| Online Access: | http://www.biomedcentral.com/1741-7007/8/5 |
| _version_ | 1818789773570998272 |
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| author | Robles Vanesa Christen Bea Raya Marina Paramonov Ida Belmonte Juan |
| author_facet | Robles Vanesa Christen Bea Raya Marina Paramonov Ida Belmonte Juan |
| author_sort | Robles Vanesa |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Dedifferentiation occurs naturally in mature cell types during epimorphic regeneration in fish and some amphibians. Dedifferentiation also occurs in the induction of pluripotent stem cells when a set of transcription factors (<it>Oct4, Sox2, Klf4 </it>and <it>c-Myc</it>) is over expressed in mature cell types.</p> <p>Results</p> <p>We hypothesised that there are parallels between dedifferentiation or reprogramming of somatic cells to induced pluripotent stem cells and the natural process of dedifferentiation during epimorphic regeneration. We analysed expression levels of the most commonly used pluripotency associated factors in regenerating and non-regenerating tissue and compared them with levels in a pluripotent reference cell. We found that some of the pluripotency associated factors (<it>oct4/pou5f1, sox2, c-myc, klf4, tert, sall4, zic3, dppa2/4 </it>and <it>fut1</it>, a homologue of <it>ssea1</it>) were expressed before and during regeneration and that at least two of these factors (<it>oct4, sox2</it>) were also required for normal fin regeneration in the zebrafish. However these factors were not upregulated during regeneration as would be expected if blastema cells acquired pluripotency.</p> <p>Conclusions</p> <p>By comparing cells from the regeneration blastema with embryonic pluripotent reference cells we found that induced pluripotent stem and blastema cells do not share pluripotency. However, during blastema formation some of the key reprogramming factors are both expressed and are also required for regeneration to take place. We therefore propose a link between partially reprogrammed induced pluripotent stem cells and the half way state of blastema cells and suggest that a common mechanism might be regulating these two processes.</p> |
| first_indexed | 2024-12-18T14:44:53Z |
| format | Article |
| id | doaj.art-b6c4725c85bd46dd9175a76e3b5f56b5 |
| institution | Directory Open Access Journal |
| issn | 1741-7007 |
| language | English |
| last_indexed | 2024-12-18T14:44:53Z |
| publishDate | 2010-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biology |
| spelling | doaj.art-b6c4725c85bd46dd9175a76e3b5f56b52022-12-21T21:04:17ZengBMCBMC Biology1741-70072010-01-0181510.1186/1741-7007-8-5Regeneration and reprogramming comparedRobles VanesaChristen BeaRaya MarinaParamonov IdaBelmonte Juan<p>Abstract</p> <p>Background</p> <p>Dedifferentiation occurs naturally in mature cell types during epimorphic regeneration in fish and some amphibians. Dedifferentiation also occurs in the induction of pluripotent stem cells when a set of transcription factors (<it>Oct4, Sox2, Klf4 </it>and <it>c-Myc</it>) is over expressed in mature cell types.</p> <p>Results</p> <p>We hypothesised that there are parallels between dedifferentiation or reprogramming of somatic cells to induced pluripotent stem cells and the natural process of dedifferentiation during epimorphic regeneration. We analysed expression levels of the most commonly used pluripotency associated factors in regenerating and non-regenerating tissue and compared them with levels in a pluripotent reference cell. We found that some of the pluripotency associated factors (<it>oct4/pou5f1, sox2, c-myc, klf4, tert, sall4, zic3, dppa2/4 </it>and <it>fut1</it>, a homologue of <it>ssea1</it>) were expressed before and during regeneration and that at least two of these factors (<it>oct4, sox2</it>) were also required for normal fin regeneration in the zebrafish. However these factors were not upregulated during regeneration as would be expected if blastema cells acquired pluripotency.</p> <p>Conclusions</p> <p>By comparing cells from the regeneration blastema with embryonic pluripotent reference cells we found that induced pluripotent stem and blastema cells do not share pluripotency. However, during blastema formation some of the key reprogramming factors are both expressed and are also required for regeneration to take place. We therefore propose a link between partially reprogrammed induced pluripotent stem cells and the half way state of blastema cells and suggest that a common mechanism might be regulating these two processes.</p>http://www.biomedcentral.com/1741-7007/8/5 |
| spellingShingle | Robles Vanesa Christen Bea Raya Marina Paramonov Ida Belmonte Juan Regeneration and reprogramming compared BMC Biology |
| title | Regeneration and reprogramming compared |
| title_full | Regeneration and reprogramming compared |
| title_fullStr | Regeneration and reprogramming compared |
| title_full_unstemmed | Regeneration and reprogramming compared |
| title_short | Regeneration and reprogramming compared |
| title_sort | regeneration and reprogramming compared |
| url | http://www.biomedcentral.com/1741-7007/8/5 |
| work_keys_str_mv | AT roblesvanesa regenerationandreprogrammingcompared AT christenbea regenerationandreprogrammingcompared AT rayamarina regenerationandreprogrammingcompared AT paramonovida regenerationandreprogrammingcompared AT belmontejuan regenerationandreprogrammingcompared |