A Novel Non-Digestible, Carrot-Derived Polysaccharide (cRG-I) Selectively Modulates the Human Gut Microbiota while Promoting Gut Barrier Integrity: An Integrated In Vitro Approach
Modulation of the gut microbiome as a means to improve human health has recently gained increasing interest. In this study, it was investigated whether cRG-I, a carrot-derived pectic polysaccharide, enriched in rhamnogalacturonan-I (RG-I) classifies as a potential prebiotic ingredient using novel in...
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| Format: | Article |
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MDPI AG
2020-06-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/7/1917 |
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| author | Pieter Van den Abbeele Lynn Verstrepen Jonas Ghyselinck Ruud Albers Massimo Marzorati Annick Mercenier |
| author_facet | Pieter Van den Abbeele Lynn Verstrepen Jonas Ghyselinck Ruud Albers Massimo Marzorati Annick Mercenier |
| author_sort | Pieter Van den Abbeele |
| collection | DOAJ |
| description | Modulation of the gut microbiome as a means to improve human health has recently gained increasing interest. In this study, it was investigated whether cRG-I, a carrot-derived pectic polysaccharide, enriched in rhamnogalacturonan-I (RG-I) classifies as a potential prebiotic ingredient using novel in vitro models. First, digestion methods involving α-amylase/brush border enzymes demonstrated the non-digestibility of cRG-I by host-derived enzymes versus digestible (starch/maltose) and non-digestible controls (inulin). Then, a recently developed short-term (48 h) colonic incubation strategy was applied and revealed that cRG-I fermentation increased levels of health-promoting short-chain fatty acids (SCFA; mainly acetate and propionate) and lactate comparable but not identical to the reference prebiotic inulin. Upon upgrading this fermentation model by inclusion of a simulated mucosal environment while applying quantitative 16S-targeted Illumina sequencing, cRG-I was additionally shown to specifically stimulate operational taxonomic units (OTUs) related to health-associated species such as <i>Bifidobacterium longum</i>, <i>Bifidobacterium adolescentis</i>, <i>Bacteroides dorei</i>, <i>Bacteroides ovatus</i>, <i>Roseburia hominis</i>, <i>Faecalibacterium prausnitzii</i>, and <i>Eubacterium hallii</i>. Finally, in a novel model to assess host–microbe interactions (Caco-2/peripheral blood mononuclear cells (PBMC) co-culture) fermented cRG-I increased barrier integrity while decreasing markers for inflammation. In conclusion, by using novel in vitro models, cRG-I was identified as a promising prebiotic candidate to proceed to clinical studies. |
| first_indexed | 2024-03-10T18:49:43Z |
| format | Article |
| id | doaj.art-b6c4f97d9f76488cb9c5b42092244d6f |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T18:49:43Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-b6c4f97d9f76488cb9c5b42092244d6f2023-11-20T05:15:39ZengMDPI AGNutrients2072-66432020-06-01127191710.3390/nu12071917A Novel Non-Digestible, Carrot-Derived Polysaccharide (cRG-I) Selectively Modulates the Human Gut Microbiota while Promoting Gut Barrier Integrity: An Integrated In Vitro ApproachPieter Van den Abbeele0Lynn Verstrepen1Jonas Ghyselinck2Ruud Albers3Massimo Marzorati4Annick Mercenier5ProDigest BV, Technologiepark 82, 9052 Ghent, BelgiumProDigest BV, Technologiepark 82, 9052 Ghent, BelgiumProDigest BV, Technologiepark 82, 9052 Ghent, BelgiumNutrileads BV, Bronland 12-N, 6708WH Wageningen, The NetherlandsProDigest BV, Technologiepark 82, 9052 Ghent, BelgiumNutrileads BV, Bronland 12-N, 6708WH Wageningen, The NetherlandsModulation of the gut microbiome as a means to improve human health has recently gained increasing interest. In this study, it was investigated whether cRG-I, a carrot-derived pectic polysaccharide, enriched in rhamnogalacturonan-I (RG-I) classifies as a potential prebiotic ingredient using novel in vitro models. First, digestion methods involving α-amylase/brush border enzymes demonstrated the non-digestibility of cRG-I by host-derived enzymes versus digestible (starch/maltose) and non-digestible controls (inulin). Then, a recently developed short-term (48 h) colonic incubation strategy was applied and revealed that cRG-I fermentation increased levels of health-promoting short-chain fatty acids (SCFA; mainly acetate and propionate) and lactate comparable but not identical to the reference prebiotic inulin. Upon upgrading this fermentation model by inclusion of a simulated mucosal environment while applying quantitative 16S-targeted Illumina sequencing, cRG-I was additionally shown to specifically stimulate operational taxonomic units (OTUs) related to health-associated species such as <i>Bifidobacterium longum</i>, <i>Bifidobacterium adolescentis</i>, <i>Bacteroides dorei</i>, <i>Bacteroides ovatus</i>, <i>Roseburia hominis</i>, <i>Faecalibacterium prausnitzii</i>, and <i>Eubacterium hallii</i>. Finally, in a novel model to assess host–microbe interactions (Caco-2/peripheral blood mononuclear cells (PBMC) co-culture) fermented cRG-I increased barrier integrity while decreasing markers for inflammation. In conclusion, by using novel in vitro models, cRG-I was identified as a promising prebiotic candidate to proceed to clinical studies.https://www.mdpi.com/2072-6643/12/7/1917prebioticmicrobiomeSCFAcolonbifidobacteriapectin |
| spellingShingle | Pieter Van den Abbeele Lynn Verstrepen Jonas Ghyselinck Ruud Albers Massimo Marzorati Annick Mercenier A Novel Non-Digestible, Carrot-Derived Polysaccharide (cRG-I) Selectively Modulates the Human Gut Microbiota while Promoting Gut Barrier Integrity: An Integrated In Vitro Approach Nutrients prebiotic microbiome SCFA colon bifidobacteria pectin |
| title | A Novel Non-Digestible, Carrot-Derived Polysaccharide (cRG-I) Selectively Modulates the Human Gut Microbiota while Promoting Gut Barrier Integrity: An Integrated In Vitro Approach |
| title_full | A Novel Non-Digestible, Carrot-Derived Polysaccharide (cRG-I) Selectively Modulates the Human Gut Microbiota while Promoting Gut Barrier Integrity: An Integrated In Vitro Approach |
| title_fullStr | A Novel Non-Digestible, Carrot-Derived Polysaccharide (cRG-I) Selectively Modulates the Human Gut Microbiota while Promoting Gut Barrier Integrity: An Integrated In Vitro Approach |
| title_full_unstemmed | A Novel Non-Digestible, Carrot-Derived Polysaccharide (cRG-I) Selectively Modulates the Human Gut Microbiota while Promoting Gut Barrier Integrity: An Integrated In Vitro Approach |
| title_short | A Novel Non-Digestible, Carrot-Derived Polysaccharide (cRG-I) Selectively Modulates the Human Gut Microbiota while Promoting Gut Barrier Integrity: An Integrated In Vitro Approach |
| title_sort | novel non digestible carrot derived polysaccharide crg i selectively modulates the human gut microbiota while promoting gut barrier integrity an integrated in vitro approach |
| topic | prebiotic microbiome SCFA colon bifidobacteria pectin |
| url | https://www.mdpi.com/2072-6643/12/7/1917 |
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